Advertisement
Research Article

Use of Genomic DNA as an Indirect Reference for Identifying Gender-Associated Transcripts in Morphologically Identical, but Chromosomally Distinct, Schistosoma mansoni Cercariae

  • Jennifer M. Fitzpatrick,

    Affiliation: Department of Pathology, University of Cambridge, Cambridge, United Kingdom

    X
  • Anna V. Protasio,

    Affiliation: Department of Pathology, University of Cambridge, Cambridge, United Kingdom

    X
  • Andrew J. McArdle,

    Affiliation: Department of Pathology, University of Cambridge, Cambridge, United Kingdom

    X
  • Gary A. Williams,

    Affiliation: Rosalind Franklin Centre for Genomics Research, Hinxton, Cambridge, United Kingdom

    X
  • David A. Johnston,

    Affiliation: Department of Zoology, The Natural History Museum, London, United Kingdom

    X
  • Karl F. Hoffmann mail

    krh@aber.ac.uk

    Affiliation: Department of Pathology, University of Cambridge, Cambridge, United Kingdom

    Current address: Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, Ceredigion, United Kingdom

    X
  • Published: October 22, 2008
  • DOI: 10.1371/journal.pntd.0000323

About the Authors

Jennifer M. Fitzpatrick, Anna V. Protasio, Andrew J. McArdle, Karl F. Hoffmann
Department of Pathology, University of Cambridge, Cambridge, United Kingdom
Gary A. Williams
Rosalind Franklin Centre for Genomics Research, Hinxton, Cambridge, United Kingdom
David A. Johnston
Department of Zoology, The Natural History Museum, London, United Kingdom

Corresponding Author

Email: krh@aber.ac.uk

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: JMF. Performed the experiments: JMF. Analyzed the data: JMF. Wrote the paper: JMF. Helped conceive the study, carried out the DNA microarray hybridization experiments and data analysis as well as helped draft the manuscript: JMF. Performed the real time PCR experiments: AVP. Wrote the scripts to link the 50-mer oligonucleotides to the Gbrowse window of SchistoGeneDB: AJM. Performed the bioinformatics analysis of the schistosome sequences used for oligonucleotide design and helped in downstream bioinformatics analyses of differentially transcribed sequences: DAJ. Designed the oligonucleotides from the assembled sequences: GAW Helped conceive the study, participated in the design and coordination, analyzed the data and helped to draft the manuscript: KFH.