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Research Article

Detection of Wuchereria bancrofti L3 Larvae in Mosquitoes: A Reverse Transcriptase PCR Assay Evaluating Infection and Infectivity

  • Sandra J. Laney mail,

    slaney@smith.edu

    Affiliations: Department of Biological Sciences, Smith College, Northampton, Massachusetts, United States of America, Zoology Department, Ain Shams University, Cairo, Egypt

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  • Reda M. R. Ramzy,

    Affiliation: National Nutrition Institute, Cairo, Egypt

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  • Hanan H. Helmy,

    Affiliation: Research and Training Center on Vectors of Diseases, Ain Shams University, Cairo, Egypt

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  • Hoda A. Farid,

    Affiliation: Department of Entomology, Ain Shams University, Cairo, Egypt

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  • Ameen A. Ashour,

    Affiliation: Faculty of Science, Taif University, Taif, Kingdom of Saudi Arabia

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  • Gary J. Weil,

    Affiliation: Infectious Disease Division, Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri, United States of America

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  • Steven A. Williams

    Affiliations: Department of Biological Sciences, Smith College, Northampton, Massachusetts, United States of America, Molecular and Cellular Biology, University of Massachusetts, Amherst, Massachusetts, United States of America

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  • Published: February 16, 2010
  • DOI: 10.1371/journal.pntd.0000602

About the Authors

Sandra J. Laney, Steven A. Williams
Department of Biological Sciences, Smith College, Northampton, Massachusetts, United States of America
Sandra J. Laney
Zoology Department, Ain Shams University, Cairo, Egypt
Reda M. R. Ramzy
National Nutrition Institute, Cairo, Egypt
Hanan H. Helmy
Research and Training Center on Vectors of Diseases, Ain Shams University, Cairo, Egypt
Hoda A. Farid
Department of Entomology, Ain Shams University, Cairo, Egypt
Ameen A. Ashour
Faculty of Science, Taif University, Taif, Kingdom of Saudi Arabia
Gary J. Weil
Infectious Disease Division, Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri, United States of America
Steven A. Williams
Molecular and Cellular Biology, University of Massachusetts, Amherst, Massachusetts, United States of America

Corresponding Author

Email: slaney@smith.edu

Competing Interests

GJW is on the Editorial Board of PLoS Neglected Tropical Diseases as an Editorial Advisor.

Author Contributions

Conceived and designed the experiments: SJL RMRR HHH HAF GJW SAW. Performed the experiments: SJL. Analyzed the data: SJL. Contributed reagents/materials/analysis tools: SJL HAF. Wrote the paper: SJL. Edited and revised the manuscript: RMRR HHH HAF AAA GJW SAW.