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Research Article

Molecular Evidence for a Functional Ecdysone Signaling System in Brugia malayi

  • George Tzertzinis mail,

    tzertzinis@neb.com

    Affiliation: New England Biolabs, Ipswich, Massachusetts, United States of America

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  • Ana L. Egaña,

    Affiliation: New England Biolabs, Ipswich, Massachusetts, United States of America

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  • Subba Reddy Palli,

    Affiliation: Department of Entomology, University of Kentucky, Lexington, Kentucky, United States of America

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  • Marc Robinson-Rechavi,

    Affiliation: Department of Ecology and Evolution, University of Lausanne, Lausanne, Switzerland

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  • Chris R. Gissendanner,

    Affiliation: New England Biolabs, Ipswich, Massachusetts, United States of America

    Current address: Department of Biology, University of Louisiana at Monroe, Monroe, Louisiana 71209, United States of America

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  • Canhui Liu,

    Affiliation: Global Health Infectious Disease Research Program, College of Public Health, University of South Florida, Tampa, Florida, United States of America

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  • Thomas R. Unnasch,

    Affiliation: Global Health Infectious Disease Research Program, College of Public Health, University of South Florida, Tampa, Florida, United States of America

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  • Claude V. Maina

    Affiliation: New England Biolabs, Ipswich, Massachusetts, United States of America

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  • Published: March 09, 2010
  • DOI: 10.1371/journal.pntd.0000625

About the Authors

George Tzertzinis, Ana L. Egaña, Chris R. Gissendanner, Claude V. Maina
New England Biolabs, Ipswich, Massachusetts, United States of America
Subba Reddy Palli
Department of Entomology, University of Kentucky, Lexington, Kentucky, United States of America
Marc Robinson-Rechavi
Department of Ecology and Evolution, University of Lausanne, Lausanne, Switzerland
Canhui Liu, Thomas R. Unnasch
Global Health Infectious Disease Research Program, College of Public Health, University of South Florida, Tampa, Florida, United States of America

Corresponding Author

Email: tzertzinis@neb.com

Competing Interests

TRU is a co-PI on a grant (supporting a different project) with one of the PLoS Neglected Tropical Diseases Deputy Editors (S. Lustigman) and is a member of the board of Editorial Advisors on the journal.

Author Contributions

Conceived and designed the experiments: GT TRU CVM. Performed the experiments: GT ALE SRP CRG CL. Analyzed the data: GT ALE MRR CRG TRU CVM. Contributed reagents/materials/analysis tools: SRP TRU. Wrote the paper: GT ALE. Cloning of BmaEcR, OvRXR, construction of plasmids, and protein-DNA binding: GT. Cloning of BmaRXR, Northern blots: ALE. Mammalian cell 2-hybrid assays: SRP. Phylogenetic analysis of sequence data: MRR. Cloning of BmaRXR: CRG. Promoter for Brugia reporter assay and Brugia biolistic transformation assays: TRU. Cloning of nematode receptors, sequence analysis: CVM.