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Research Article

Diagnostic Accuracy of the Leishmania OligoC-TesT and NASBA-Oligochromatography for Diagnosis of Leishmaniasis in Sudan

  • Alfarazdeg A. Saad,

    Affiliation: Department of Biochemistry, Khartoum University, Sudan

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  • Nuha G. Ahmed,

    Affiliation: Department of Biochemistry, Khartoum University, Sudan

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  • Osman S. Osman,

    Affiliation: Department of Biochemistry, Khartoum University, Sudan

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  • Ahmed Almustafa Al-Basheer,

    Affiliation: Department of Microbiology and Parasitology, Khartoum University, Sudan

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  • Awad Hamad,

    Affiliation: Department of Microbiology and Parasitology, Khartoum University, Sudan

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  • Stijn Deborggraeve mail,

    sdeborggraeve@itg.be

    Affiliations: Department of Parasitology, Institute of Tropical Medicine, Antwerp, Belgium, Rega Institute, Katholieke Universiteit Leuven, Leuven, Belgium

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  • Philippe Büscher,

    Affiliation: Department of Parasitology, Institute of Tropical Medicine, Antwerp, Belgium

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  • Gerard J. Schoone,

    Affiliation: KIT Biomedical Research, Parasitology Unit, Koninklijk Instituut voor de Tropen (KIT), Amsterdam, The Netherlands

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  • Henk D. Schallig,

    Affiliation: KIT Biomedical Research, Parasitology Unit, Koninklijk Instituut voor de Tropen (KIT), Amsterdam, The Netherlands

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  • Thierry Laurent,

    Affiliation: Coris BioConcept, Gembloux, Belgium

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  • Ahmed Haleem,

    Affiliation: Faculty of Laboratory Science, Khartoum University, Sudan

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  • Omran F. Osman,

    Affiliation: Faculty of Science, Khartoum University, Sudan

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  • Ahmed Mohamedain Eltom,

    Affiliation: Department of Biochemistry, Khartoum University, Sudan

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  • Mustafa I. Elbashir,

    Affiliation: Department of Biochemistry, Khartoum University, Sudan

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  • Sayda El-Safi

    Affiliation: Department of Microbiology and Parasitology, Khartoum University, Sudan

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  • Published: August 03, 2010
  • DOI: 10.1371/journal.pntd.0000776

About the Authors

Alfarazdeg A. Saad, Nuha G. Ahmed, Osman S. Osman, Ahmed Mohamedain Eltom, Mustafa I. Elbashir
Department of Biochemistry, Khartoum University, Sudan
Ahmed Almustafa Al-Basheer, Awad Hamad, Sayda El-Safi
Department of Microbiology and Parasitology, Khartoum University, Sudan
Stijn Deborggraeve, Philippe Büscher
Department of Parasitology, Institute of Tropical Medicine, Antwerp, Belgium
Stijn Deborggraeve
Rega Institute, Katholieke Universiteit Leuven, Leuven, Belgium
Gerard J. Schoone, Henk D. Schallig
KIT Biomedical Research, Parasitology Unit, Koninklijk Instituut voor de Tropen (KIT), Amsterdam, The Netherlands
Thierry Laurent
Coris BioConcept, Gembloux, Belgium
Ahmed Haleem
Faculty of Laboratory Science, Khartoum University, Sudan
Omran F. Osman
Faculty of Science, Khartoum University, Sudan

Corresponding Author

Email: sdeborggraeve@itg.be

Competing Interests

T. Laurent is an employee of Coris BioConcept, the company involved in the development and provisioning of the OligoC-TesTs. All other authors report no potential conflicts.

Author Contributions

Conceived and designed the experiments: O. S. Osman, A. A. Al-Basheer, S. Deborggraeve, P. Büscher, G. J. Schoone, H. D. Schallig, T. Laurent, O. F. Osman, A. M. Eltom, M. I. Elbashir, S. El-Safi. Performed the experiments: A. A. Saad, N. G. Ahmed, A. Hamad, A. Haleem. Analyzed the data: A. A. Saad, S. Deborggraeve, S. El-Safi. Contributed reagents/materials/analysis tools: G. J. Schoone, T. Laurent, S. El-Safi. Wrote the paper: A. A. Saad, N. G. Ahmed, O. S. Osman, A. A. Al-Bashir, A. Hamad, S. Deborggraeve, P. Büscher, G. J. Schoone, H. D. Schallig, T. Laurent, A. Haleem, O. F. Osman, A. M. Eltom, M. I. Elbashir, S. El-Safi.