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Research Article

RNA Interference in Schistosoma mansoni Schistosomula: Selectivity, Sensitivity and Operation for Larger-Scale Screening

  • Saša Štefanić equal contributor,

    equal contributor Contributed equally to this work with: Saša Štefanić, Jan Dvořák

    Affiliation: Sandler Center for Drug Discovery, California Institute for Quantitative Biosciences (QB3), University of California San Francisco, San Francisco, California, United States of America

    Current address: Institute of Parasitology, University of Zurich, Zurich, Switzerland

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  • Jan Dvořák equal contributor,

    equal contributor Contributed equally to this work with: Saša Štefanić, Jan Dvořák

    Affiliation: Sandler Center for Drug Discovery, California Institute for Quantitative Biosciences (QB3), University of California San Francisco, San Francisco, California, United States of America

    Current address: Institute of Parasitology, Biology Centre, Academy of Sciences of the Czech Republic, České Budějovice Czech Republic

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  • Martin Horn,

    Affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Praha, Czech Republic

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  • Simon Braschi,

    Affiliation: Sandler Center for Drug Discovery, California Institute for Quantitative Biosciences (QB3), University of California San Francisco, San Francisco, California, United States of America

    Current address: Abcam Plc, Cambridge, United Kingdom

    X
  • Daniel Sojka,

    Affiliation: Institute of Parasitology, Biology Centre, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic

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  • Debbie S. Ruelas,

    Affiliation: Sandler Center for Drug Discovery, California Institute for Quantitative Biosciences (QB3), University of California San Francisco, San Francisco, California, United States of America

    Current address: Gladstone Institute of Virology and Immunology, San Francisco, California, United States of America

    X
  • Brian Suzuki,

    Affiliation: Sandler Center for Drug Discovery, California Institute for Quantitative Biosciences (QB3), University of California San Francisco, San Francisco, California, United States of America

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  • Kee-Chong Lim,

    Affiliation: Sandler Center for Drug Discovery, California Institute for Quantitative Biosciences (QB3), University of California San Francisco, San Francisco, California, United States of America

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  • Stephanie D. Hopkins,

    Affiliation: Sandler Center for Drug Discovery, California Institute for Quantitative Biosciences (QB3), University of California San Francisco, San Francisco, California, United States of America

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  • James H. McKerrow,

    Affiliation: Sandler Center for Drug Discovery, California Institute for Quantitative Biosciences (QB3), University of California San Francisco, San Francisco, California, United States of America

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  • Conor R. Caffrey mail

    conor.caffrey@ucsf.edu

    Affiliation: Sandler Center for Drug Discovery, California Institute for Quantitative Biosciences (QB3), University of California San Francisco, San Francisco, California, United States of America

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  • Published: October 19, 2010
  • DOI: 10.1371/journal.pntd.0000850

About the Authors

Saša Štefanić, Jan Dvořák, Simon Braschi, Debbie S. Ruelas, Brian Suzuki, Kee-Chong Lim, Stephanie D. Hopkins, James H. McKerrow, Conor R. Caffrey
Sandler Center for Drug Discovery, California Institute for Quantitative Biosciences (QB3), University of California San Francisco, San Francisco, California, United States of America
Martin Horn
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Praha, Czech Republic
Daniel Sojka
Institute of Parasitology, Biology Centre, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic

Corresponding Author

Email: conor.caffrey@ucsf.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: SŠ JD MH SB DS CRC. Performed the experiments: SŠ JD MH SB DS DSR BS K-CL SDH CRC. Analyzed the data: SŠ JD MH SB DS DSR BS CRC. Wrote the paper: SŠ JD MH JHM CRC.