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Research Article

Using Detergent to Enhance Detection Sensitivity of African Trypanosomes in Human CSF and Blood by Loop-Mediated Isothermal Amplification (LAMP)

  • Dennis J. Grab mail,

    dgrab1@jhmi.edu

    Affiliation: Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America

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  • Olga V. Nikolskaia,

    Affiliation: Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America

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  • Noboru Inoue,

    Affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan

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  • Oriel M. M. Thekisoe,

    Affiliation: Department of Zoology and Entomology, University of the Free State, Qwaqwa Campus, Phuthaditjhaba, South Africa

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  • Liam J. Morrison,

    Affiliation: Wellcome Trust Centre for Molecular Parasitology, University of Glasgow, Glasgow, United Kingdom

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  • Wendy Gibson,

    Affiliation: School of Biological Sciences, University of Bristol, Bristol, United Kingdom

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  • J. Stephen Dumler

    Affiliation: Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America

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  • Published: August 02, 2011
  • DOI: 10.1371/journal.pntd.0001249

About the Authors

Dennis J. Grab, Olga V. Nikolskaia, J. Stephen Dumler
Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
Noboru Inoue
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan
Oriel M. M. Thekisoe
Department of Zoology and Entomology, University of the Free State, Qwaqwa Campus, Phuthaditjhaba, South Africa
Liam J. Morrison
Wellcome Trust Centre for Molecular Parasitology, University of Glasgow, Glasgow, United Kingdom
Wendy Gibson
School of Biological Sciences, University of Bristol, Bristol, United Kingdom

Corresponding Author

Email: dgrab1@jhmi.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: DJG NI LJM JSD. Performed the experiments: OVN LJM NI. Analyzed the data: DJG WG NI LJM JSD OMMT. Contributed reagents/materials/analysis tools: DJG NI. Wrote the paper: DJG WG LJM JSD NI OMMT.