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Research Article

Real-time PCR Demonstrates Ancylostoma duodenale Is a Key Factor in the Etiology of Severe Anemia and Iron Deficiency in Malawian Pre-school Children

  • Femkje A. M. Jonker mail,

    femkje.jonker@live.nl

    Affiliation: Global Child Health Group, Emma Children's Hospital, Academic Medical Centre, Amsterdam, The Netherlands

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  • Job C. J. Calis,

    Affiliation: Global Child Health Group, Emma Children's Hospital, Academic Medical Centre, Amsterdam, The Netherlands

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  • Kamija Phiri,

    Affiliation: Community Health Department, College of Medicine, Blantyre, Malawi

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  • Eric A. T. Brienen,

    Affiliation: Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands

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  • Harriet Khoffi,

    Affiliation: Community Health Department, College of Medicine, Blantyre, Malawi

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  • Bernard J. Brabin,

    Affiliation: Global Child Health Group, Emma Children's Hospital, Academic Medical Centre, Amsterdam, The Netherlands

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  • Jaco J. Verweij,

    Affiliation: Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands

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  • Michael Boele van Hensbroek,

    Affiliation: Global Child Health Group, Emma Children's Hospital, Academic Medical Centre, Amsterdam, The Netherlands

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  • Lisette van Lieshout

    Affiliation: Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands

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  • Published: March 06, 2012
  • DOI: 10.1371/journal.pntd.0001555

About the Authors

Femkje A. M. Jonker, Job C. J. Calis, Bernard J. Brabin, Michael Boele van Hensbroek
Global Child Health Group, Emma Children's Hospital, Academic Medical Centre, Amsterdam, The Netherlands
Kamija Phiri, Harriet Khoffi
Community Health Department, College of Medicine, Blantyre, Malawi
Eric A. T. Brienen, Jaco J. Verweij, Lisette van Lieshout
Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands

Corresponding Author

Email: femkje.jonker@live.nl

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: MBvH LvL. Performed the experiments: JCJC KP EATB HK BJB JJV MBvH LvL. Analyzed the data: FAMJ JCJC LvL. Contributed reagents/materials/analysis tools: JCJC KP EATB HK BJB JJV MBvH LvL. Wrote the paper: FAMJ JCJC KP HK BJB JJV MBvH LvL.