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Research Article

Molecular Diagnostics for Lassa Fever at Irrua Specialist Teaching Hospital, Nigeria: Lessons Learnt from Two Years of Laboratory Operation

  • Danny A. Asogun equal contributor mail,

    equal contributor Contributed equally to this work with: Danny A. Asogun, Donatus I. Adomeh, Jacqueline Ehimuan, Ikponmwonsa Odia

    asogun2001@yahoo.com (DAA); guenther@bni.uni-hamburg.de (SG)

    Affiliation: Institute of Lassa Fever Research and Control, Irrua Specialist Teaching Hospital, Edo State, Nigeria

    X
  • Donatus I. Adomeh equal contributor,

    equal contributor Contributed equally to this work with: Danny A. Asogun, Donatus I. Adomeh, Jacqueline Ehimuan, Ikponmwonsa Odia

    Affiliation: Institute of Lassa Fever Research and Control, Irrua Specialist Teaching Hospital, Edo State, Nigeria

    X
  • Jacqueline Ehimuan equal contributor,

    equal contributor Contributed equally to this work with: Danny A. Asogun, Donatus I. Adomeh, Jacqueline Ehimuan, Ikponmwonsa Odia

    Affiliation: Institute of Lassa Fever Research and Control, Irrua Specialist Teaching Hospital, Edo State, Nigeria

    X
  • Ikponmwonsa Odia equal contributor,

    equal contributor Contributed equally to this work with: Danny A. Asogun, Donatus I. Adomeh, Jacqueline Ehimuan, Ikponmwonsa Odia

    Affiliation: Institute of Lassa Fever Research and Control, Irrua Specialist Teaching Hospital, Edo State, Nigeria

    X
  • Meike Hass,

    Affiliation: Department of Virology, Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany

    X
  • Martin Gabriel,

    Affiliation: Department of Virology, Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany

    X
  • Stephan Ölschläger,

    Affiliation: Department of Virology, Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany

    X
  • Beate Becker-Ziaja,

    Affiliation: Department of Virology, Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany

    X
  • Onikepe Folarin,

    Affiliation: Malaria Research Laboratories, College of Medicine, University of Ibadan, Ibadan, Nigeria

    X
  • Eric Phelan,

    Affiliations: Broad Institute, Cambridge, Massachusetts, United States of America, Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, United States of America

    X
  • Philomena E. Ehiane,

    Affiliation: Institute of Lassa Fever Research and Control, Irrua Specialist Teaching Hospital, Edo State, Nigeria

    X
  • Veritas E. Ifeh,

    Affiliation: Institute of Lassa Fever Research and Control, Irrua Specialist Teaching Hospital, Edo State, Nigeria

    X
  • Eghosasere A. Uyigue,

    Affiliation: Institute of Lassa Fever Research and Control, Irrua Specialist Teaching Hospital, Edo State, Nigeria

    X
  • Yemisi T. Oladapo,

    Affiliation: Institute of Lassa Fever Research and Control, Irrua Specialist Teaching Hospital, Edo State, Nigeria

    X
  • Ekene B. Muoebonam,

    Affiliation: Institute of Lassa Fever Research and Control, Irrua Specialist Teaching Hospital, Edo State, Nigeria

    X
  • Osagie Osunde,

    Affiliation: Institute of Lassa Fever Research and Control, Irrua Specialist Teaching Hospital, Edo State, Nigeria

    X
  • Andrew Dongo,

    Affiliation: Accident and Emergency Unit, Irrua Specialist Teaching Hospital, Edo State, Nigeria

    X
  • Peter O. Okokhere,

    Affiliation: Department of Medicine, Irrua Specialist Teaching Hospital, Edo State, Nigeria

    X
  • Sylvanus A. Okogbenin,

    Affiliation: Department of Obstetrics and Gynecology, Irrua Specialist Teaching Hospital, Edo State, Nigeria

    X
  • Mojeed Momoh,

    Affiliation: Department of Obstetrics and Gynecology, Irrua Specialist Teaching Hospital, Edo State, Nigeria

    X
  • Sylvester O. Alikah,

    Affiliation: Department of Pediatrics, Irrua Specialist Teaching Hospital, Edo State, Nigeria

    X
  • Odigie C. Akhuemokhan,

    Affiliation: Department of Pediatrics, Irrua Specialist Teaching Hospital, Edo State, Nigeria

    X
  • Peter Imomeh,

    Affiliation: Department of Family Medicine, Irrua Specialist Teaching Hospital, Edo State, Nigeria

    X
  • Maxy A. C. Odike,

    Affiliation: Department of Clinical Pathology, Irrua Specialist Teaching Hospital, Edo State, Nigeria

    X
  • Stephen Gire,

    Affiliations: Broad Institute, Cambridge, Massachusetts, United States of America, Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, United States of America

    X
  • Kristian Andersen,

    Affiliations: Broad Institute, Cambridge, Massachusetts, United States of America, Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, United States of America

    X
  • Pardis C. Sabeti,

    Affiliations: Broad Institute, Cambridge, Massachusetts, United States of America, Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, United States of America

    X
  • Christian T. Happi,

    Affiliation: Malaria Research Laboratories, College of Medicine, University of Ibadan, Ibadan, Nigeria

    X
  • George O. Akpede,

    Affiliation: Department of Pediatrics, Irrua Specialist Teaching Hospital, Edo State, Nigeria

    X
  • Stephan Günther mail

    asogun2001@yahoo.com (DAA); guenther@bni.uni-hamburg.de (SG)

    Affiliation: Department of Virology, Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany

    X
  • Published: September 27, 2012
  • DOI: 10.1371/journal.pntd.0001839

About the Authors

Danny A. Asogun, Donatus I. Adomeh, Jacqueline Ehimuan, Ikponmwonsa Odia, Philomena E. Ehiane, Veritas E. Ifeh, Eghosasere A. Uyigue, Yemisi T. Oladapo, Ekene B. Muoebonam, Osagie Osunde
Institute of Lassa Fever Research and Control, Irrua Specialist Teaching Hospital, Edo State, Nigeria
Meike Hass, Martin Gabriel, Stephan Ölschläger, Beate Becker-Ziaja, Stephan Günther
Department of Virology, Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany
Onikepe Folarin, Christian T. Happi
Malaria Research Laboratories, College of Medicine, University of Ibadan, Ibadan, Nigeria
Eric Phelan, Stephen Gire, Kristian Andersen, Pardis C. Sabeti
Broad Institute, Cambridge, Massachusetts, United States of America
Eric Phelan, Stephen Gire, Kristian Andersen, Pardis C. Sabeti
Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, United States of America
Andrew Dongo
Accident and Emergency Unit, Irrua Specialist Teaching Hospital, Edo State, Nigeria
Peter O. Okokhere
Department of Medicine, Irrua Specialist Teaching Hospital, Edo State, Nigeria
Sylvanus A. Okogbenin, Mojeed Momoh
Department of Obstetrics and Gynecology, Irrua Specialist Teaching Hospital, Edo State, Nigeria
Sylvester O. Alikah, Odigie C. Akhuemokhan, George O. Akpede
Department of Pediatrics, Irrua Specialist Teaching Hospital, Edo State, Nigeria
Peter Imomeh
Department of Family Medicine, Irrua Specialist Teaching Hospital, Edo State, Nigeria
Maxy A. C. Odike
Department of Clinical Pathology, Irrua Specialist Teaching Hospital, Edo State, Nigeria

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: DAA MH SÖ AD POO SAO MM PCS CTH GOA SG. Performed the experiments: DIA JE IO BB-Z PEE VEI EAU YTO EBM OO AD POO SAO SOA OCA PI MACO GOA. Analyzed the data: DAA DIA JE IO MH MG SÖ SG. Contributed reagents/materials/analysis tools: MH MG SÖ BB-Z OF EP SG KA PCS CTH SG. Wrote the paper: DAA SG.