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Research Article

Accuracy of a Dual Path Platform (DPP) Assay for the Rapid Point-of-Care Diagnosis of Human Leptospirosis

  • Scott A. Nabity,

    Affiliations: Duke University School of Medicine, Durham, North Carolina, United States of America, Centro de Pesquisa de Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Brazil

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  • Guilherme S. Ribeiro mail,

    gsribeiro@ufba.br

    Affiliations: Centro de Pesquisa de Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Brazil, Instituto de Saúde Coletiva, Universidade Federal da Bahia, Salvador, Brazil

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  • Carolina Lessa Aquino,

    Affiliation: Bio-Manguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil

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  • Daniele Takahashi,

    Affiliation: Centro de Pesquisa de Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Brazil

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  • Alcinéia Oliveira Damião,

    Affiliation: Centro de Pesquisa de Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Brazil

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  • André H. O. Gonçalves,

    Affiliation: Centro de Pesquisa de Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Brazil

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  • Demócrito B. Miranda-Filho,

    Affiliation: Departamento de Medicina Clínica, Universidade de Pernambuco, Recife, Brazil

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  • Rena Greenwald,

    Affiliation: Chembio Diagnostic Systems, Medford, New York, United States of America

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  • Javan Esfandiari,

    Affiliation: Chembio Diagnostic Systems, Medford, New York, United States of America

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  • Konstantin P. Lyashchenko,

    Affiliation: Chembio Diagnostic Systems, Medford, New York, United States of America

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  • Mitermayer G. Reis,

    Affiliation: Centro de Pesquisa de Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Brazil

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  • Marco A. Medeiros,

    Affiliation: Bio-Manguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil

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  • Albert I. Ko

    Affiliations: Centro de Pesquisa de Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Brazil, Yale University Schools of Public Health and Medicine, New Haven, Connecticut, United States of America

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  • Published: November 01, 2012
  • DOI: 10.1371/journal.pntd.0001878

About the Authors

Scott A. Nabity
Duke University School of Medicine, Durham, North Carolina, United States of America
Scott A. Nabity, Guilherme S. Ribeiro, Daniele Takahashi, Alcinéia Oliveira Damião, André H. O. Gonçalves, Mitermayer G. Reis, Albert I. Ko
Centro de Pesquisa de Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Brazil
Guilherme S. Ribeiro
Instituto de Saúde Coletiva, Universidade Federal da Bahia, Salvador, Brazil
Carolina Lessa Aquino, Marco A. Medeiros
Bio-Manguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil
Demócrito B. Miranda-Filho
Departamento de Medicina Clínica, Universidade de Pernambuco, Recife, Brazil
Rena Greenwald, Javan Esfandiari, Konstantin P. Lyashchenko
Chembio Diagnostic Systems, Medford, New York, United States of America
Albert I. Ko
Yale University Schools of Public Health and Medicine, New Haven, Connecticut, United States of America

Corresponding Author

Email: gsribeiro@ufba.br

Competing Interests

The authors have read the journal's policy and have the following conflicts: AIK and MGR are holders of the U.S. patent 8,124,110 for the proteins with repetitive bacterial-Ig-like (Big) domains present in Leptospira species used as antigens in the DPP assay. JE holds U.S. patent 7,879,597 for the dual path immunoassay device (DPP) evaluated in this study. JE, KPL, and RG are employees of Chembio Diagnostic Systems, Inc., which developed the DPP assay for leptospirosis and has transferred the DPP technology to the Brazilian Ministry of Health.

Author Contributions

Conceived and designed the experiments: SAN GSR CLA DT KPL MAM AIK. Performed the experiments: SAN CLA DT AOD AHOG. Analyzed the data: SAN GSR KPL AIK. Contributed reagents/materials/analysis tools: DBMF RG JE KPL MGR MAM AIK. Wrote the paper: SAN GSR KPL. Reviewed and approved the final version of the manuscript: SAN GSR CLA DT AOD AHOG DBMF RG JE KPL MGR MAM AIK.