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Research Article

Analytical and Clinical Performance of the CDC Real Time RT-PCR Assay for Detection and Typing of Dengue Virus

  • Gilberto A. Santiago,

    Affiliation: Centers for Disease Control and Prevention, Division of Vector-Borne Diseases, Dengue Branch, San Juan, Puerto Rico, United States of America

    X
  • Edgardo Vergne,

    Affiliation: Centers for Disease Control and Prevention, Division of Vector-Borne Diseases, Dengue Branch, San Juan, Puerto Rico, United States of America

    X
  • Yashira Quiles,

    Affiliation: Centers for Disease Control and Prevention, Division of Vector-Borne Diseases, Dengue Branch, San Juan, Puerto Rico, United States of America

    X
  • Joan Cosme,

    Affiliation: Centers for Disease Control and Prevention, Division of Vector-Borne Diseases, Dengue Branch, San Juan, Puerto Rico, United States of America

    X
  • Jesus Vazquez,

    Affiliation: Centers for Disease Control and Prevention, Division of Vector-Borne Diseases, Dengue Branch, San Juan, Puerto Rico, United States of America

    X
  • Juan F. Medina,

    Affiliation: Centers for Disease Control and Prevention, Division of Vector-Borne Diseases, Dengue Branch, San Juan, Puerto Rico, United States of America

    X
  • Freddy Medina,

    Affiliation: Centers for Disease Control and Prevention, Division of Vector-Borne Diseases, Dengue Branch, San Juan, Puerto Rico, United States of America

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  • Candimar Colón,

    Affiliation: Centers for Disease Control and Prevention, Division of Vector-Borne Diseases, Dengue Branch, San Juan, Puerto Rico, United States of America

    X
  • Harold Margolis,

    Affiliation: Centers for Disease Control and Prevention, Division of Vector-Borne Diseases, Dengue Branch, San Juan, Puerto Rico, United States of America

    X
  • Jorge L. Muñoz-Jordán mail

    JMunoz@cdc.gov

    Affiliation: Centers for Disease Control and Prevention, Division of Vector-Borne Diseases, Dengue Branch, San Juan, Puerto Rico, United States of America

    X
  • Published: July 11, 2013
  • DOI: 10.1371/journal.pntd.0002311

About the Authors

Gilberto A. Santiago, Edgardo Vergne, Yashira Quiles, Joan Cosme, Jesus Vazquez, Juan F. Medina, Freddy Medina, Candimar Colón, Harold Margolis, Jorge L. Muñoz-Jordán
Centers for Disease Control and Prevention, Division of Vector-Borne Diseases, Dengue Branch, San Juan, Puerto Rico, United States of America

Corresponding Author

Email: JMunoz@cdc.gov

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: GAS EV JLMJ. Performed the experiments: GAS EV YQ JC JV JFM FM. Analyzed the data: GAS EV FM CC. Contributed reagents/materials/analysis tools: CC HM. Wrote the paper: GAS HM JLMJ.