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Research Article

Use of a Synthetic Biosensor for Neutralizing Activity-Biased Selection of Monoclonal Antibodies against Atroxlysin-I, an Hemorrhagic Metalloproteinase from Bothrops atrox Snake Venom

  • Francisco Santos Schneider,

    Affiliation: Departamento de Bioquímica e Imunologia, Instituto Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil

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  • Dung Le Nguyen,

    Affiliation: SysDiag, UMR3145,CNRS/BioRad, Montpellier, France

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  • Karen Larissa Castro,

    Affiliation: Departamento de Bioquímica e Imunologia, Instituto Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil

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  • Sandra Cobo,

    Affiliation: SysDiag, UMR3145,CNRS/BioRad, Montpellier, France

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  • Ricardo Andrez Machado de Avila,

    Affiliation: Departamento de Bioquímica e Imunologia, Instituto Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil

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  • Nivia de Assis Ferreira,

    Affiliation: Departamento de Bioquímica e Imunologia, Instituto Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil

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  • Eladio Flores Sanchez,

    Affiliation: Departamento de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias, Belo Horizonte, Brasil

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  • Christophe Nguyen,

    Affiliation: SysDiag, UMR3145,CNRS/BioRad, Montpellier, France

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  • Claude Granier,

    Affiliation: SysDiag, UMR3145,CNRS/BioRad, Montpellier, France

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  • Pascale Galéa,

    Affiliation: SysDiag, UMR3145,CNRS/BioRad, Montpellier, France

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  • Carlos Chávez-Olortegui mail,

    olortegi@icb.ufmg.br

    Affiliation: Departamento de Bioquímica e Imunologia, Instituto Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil

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  • Franck Molina

    Affiliation: SysDiag, UMR3145,CNRS/BioRad, Montpellier, France

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  • Published: April 24, 2014
  • DOI: 10.1371/journal.pntd.0002826

About the Authors

Francisco Santos Schneider, Karen Larissa Castro, Ricardo Andrez Machado de Avila, Nivia de Assis Ferreira, Carlos Chávez-Olortegui
Departamento de Bioquímica e Imunologia, Instituto Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil
Dung Le Nguyen, Sandra Cobo, Christophe Nguyen, Claude Granier, Pascale Galéa, Franck Molina
SysDiag, UMR3145,CNRS/BioRad, Montpellier, France
Eladio Flores Sanchez
Departamento de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias, Belo Horizonte, Brasil

Corresponding Author

Email: olortegi@icb.ufmg.br

Competing Interests

I have read the journal's policy and have the following conflicts: The authors Sandra Cobo and Pascale Galéa are employed by the comercial part (BioRad) of the mix enterprise SysDiag. In addition, mAbatrs are in the patent application process. This does not alter our adherence to all PLOS NTDs policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: FSS DLN PG CCO FM. Performed the experiments: FSS DLN KLC SC RAMdA NdAF CN. Analyzed the data: FSS KLC SC RAMdA NdAF EFS CN CG PG CCO FM. Contributed reagents/materials/analysis tools: DLN EFS PG CCO FM. Wrote the paper: FSS DLN RAMdA EFS CN CG PG CCO FM.