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Research Article

A FRET-Based Real-Time PCR Assay to Identify the Main Causal Agents of New World Tegumentary Leishmaniasis

  • Pablo Tsukayama equal contributor,

    equal contributor Contributed equally to this work with: Pablo Tsukayama, Jorge H. Núñez

    Affiliation: Department of Parasitology, U.S. Naval Medical Research Unit No. 6 (NAMRU-6), Lima, Peru

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  • Jorge H. Núñez equal contributor,

    equal contributor Contributed equally to this work with: Pablo Tsukayama, Jorge H. Núñez

    Affiliation: Department of Parasitology, U.S. Naval Medical Research Unit No. 6 (NAMRU-6), Lima, Peru

    X
  • Maxy De Los Santos,

    Affiliation: Department of Parasitology, U.S. Naval Medical Research Unit No. 6 (NAMRU-6), Lima, Peru

    X
  • Valeria Soberón,

    Affiliation: Department of Parasitology, U.S. Naval Medical Research Unit No. 6 (NAMRU-6), Lima, Peru

    X
  • Carmen M. Lucas,

    Affiliation: Department of Parasitology, U.S. Naval Medical Research Unit No. 6 (NAMRU-6), Lima, Peru

    X
  • Greg Matlashewski,

    Affiliation: Department of Microbiology and Immunology, McGill University, Quebec, Canada

    X
  • Alejandro Llanos-Cuentas,

    Affiliation: Leishmaniasis Working Group, Instituto de Medicina Tropical ‘Alexander von Humboldt’, Universidad Peruana Cayetano Heredia, Lima, Peru

    X
  • Marianela Ore,

    Affiliation: Hospital Militar Central, Lima, Perú

    X
  • G. Christian Baldeviano,

    Affiliation: Department of Parasitology, U.S. Naval Medical Research Unit No. 6 (NAMRU-6), Lima, Peru

    X
  • Kimberly A. Edgel,

    Affiliation: Department of Parasitology, U.S. Naval Medical Research Unit No. 6 (NAMRU-6), Lima, Peru

    X
  • Andres G. Lescano mail,

    willy.lescano@med.navy.mil

    Affiliation: Department of Parasitology, U.S. Naval Medical Research Unit No. 6 (NAMRU-6), Lima, Peru

    X
  • Paul C. F. Graf,

    Affiliations: Department of Parasitology, U.S. Naval Medical Research Unit No. 6 (NAMRU-6), Lima, Peru, Microbiology, Immunology, and Molecular Diagnostics Laboratory Department, Naval Medical Center San Diego, San Diego, California, United States of America

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  • David J. Bacon

    Affiliations: Department of Parasitology, U.S. Naval Medical Research Unit No. 6 (NAMRU-6), Lima, Peru, Naval Research Laboratory, Washington, D.C., United States of America

    X
  • Published: January 03, 2013
  • DOI: 10.1371/journal.pntd.0001956

About the Authors

Pablo Tsukayama, Jorge H. Núñez, Maxy De Los Santos, Valeria Soberón, Carmen M. Lucas, G. Christian Baldeviano, Kimberly A. Edgel, Andres G. Lescano, Paul C. F. Graf, David J. Bacon
Department of Parasitology, U.S. Naval Medical Research Unit No. 6 (NAMRU-6), Lima, Peru
Greg Matlashewski
Department of Microbiology and Immunology, McGill University, Quebec, Canada
Alejandro Llanos-Cuentas
Leishmaniasis Working Group, Instituto de Medicina Tropical ‘Alexander von Humboldt’, Universidad Peruana Cayetano Heredia, Lima, Peru
Marianela Ore
Hospital Militar Central, Lima, Perú
Paul C. F. Graf
Microbiology, Immunology, and Molecular Diagnostics Laboratory Department, Naval Medical Center San Diego, San Diego, California, United States of America
David J. Bacon
Naval Research Laboratory, Washington, D.C., United States of America

Corresponding Author

Email: willy.lescano@med.navy.mil

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: PT JHN GM PCFG DJB. Performed the experiments: PT JHN MDS VS. Analyzed the data: PT JHN MDS VS CML GCB KAE AGL PCFG DJB. Contributed reagents/materials/analysis tools: AL GM MO. Wrote the paper: PT JHN GCB KAE AGL PCFG DJB.