Advertisement
Research Article

Indirect Immunofluorescence Assay for the Simultaneous Detection of Antibodies against Clinically Important Old and New World Hantaviruses

  • Sabine Lederer,

    Affiliation: EUROIMMUN Medizinische Labordiagnostika AG, Luebeck, Germany

    X
  • Erik Lattwein mail,

    e.lattwein@euroimmun.de

    Affiliation: EUROIMMUN Medizinische Labordiagnostika AG, Luebeck, Germany

    X
  • Merle Hanke,

    Affiliation: EUROIMMUN Medizinische Labordiagnostika AG, Luebeck, Germany

    X
  • Karen Sonnenberg,

    Affiliation: EUROIMMUN Medizinische Labordiagnostika AG, Luebeck, Germany

    Current address: ks-ls, Berlin, Germany

    X
  • Winfried Stoecker,

    Affiliation: EUROIMMUN Medizinische Labordiagnostika AG, Luebeck, Germany

    X
  • Åke Lundkvist,

    Affiliation: Swedish Institute for Infectious Disease Control, Karolinska Institute, Stockholm, Sweden

    X
  • Antti Vaheri,

    Affiliation: Department of Virology, Haartman Institute, University of Helsinki, Helsinki, Finland

    X
  • Olli Vapalahti,

    Affiliation: Department of Virology, Haartman Institute, University of Helsinki, Helsinki, Finland

    X
  • Paul K. S. Chan,

    Affiliation: Department of Microbiology, Faculty of Medicine, Chinese University of Hong Kong, Hong Kong, People's Republic of China

    X
  • Heinz Feldmann,

    Affiliation: Special Pathogens Program, Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada, Canadian Science Centre for Human and Animal Health, Winnipeg, Manitoba, Canada

    Current address: Laboratory of Virology, Division of Intramural Research, NIAID, NIH, Rocky Mountain Laboratories, Hamilton, Montana, United States of America

    X
  • Daryl Dick,

    Affiliation: Special Pathogens Program, Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada, Canadian Science Centre for Human and Animal Health, Winnipeg, Manitoba, Canada

    X
  • Jonas Schmidt-Chanasit,

    Affiliation: WHO Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and Research, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany

    X
  • Paula Padula,

    Affiliation: Departamento de Virología, Instituto Nacional de Enfermedades Infecciosas, A.N.L.I.S. ‘Dr. Carlos G. Malbrán,’ Buenos Aires, Argentina

    X
  • Pablo A. Vial,

    Affiliation: Clínica Alemana School of Medicine, Institute of Science, Universidad del Desarrollo, Santiago, Chile

    X
  • Raluca Panculescu-Gatej,

    Affiliation: Laboratory of Vector-Borne Infections and Medical Entomology, CANTACUZINO National Institute for Research and Development in Microbiology and Immunology, Bucharest, Romania

    X
  • Cornelia Ceianu,

    Affiliation: Laboratory of Vector-Borne Infections and Medical Entomology, CANTACUZINO National Institute for Research and Development in Microbiology and Immunology, Bucharest, Romania

    X
  • Paul Heyman,

    Affiliation: Research Laboratory for Vector-Borne Diseases, Queen Astrid Military Hospital, Brussels, Belgium

    X
  • Tatjana Avšič-Županc,

    Affiliation: Institute of Microbiology and Immunology, Laboratory for Diagnosis of Zoonoses and WHO Laboratory, Ljubljana, Slovenia

    X
  • Matthias Niedrig

    Affiliation: Center for Biological Safety (ZBS-1), Robert Koch Institute, Berlin, Germany

    X
  • Published: April 04, 2013
  • DOI: 10.1371/journal.pntd.0002157

About the Authors

Sabine Lederer, Erik Lattwein, Merle Hanke, Karen Sonnenberg, Winfried Stoecker
EUROIMMUN Medizinische Labordiagnostika AG, Luebeck, Germany
Åke Lundkvist
Swedish Institute for Infectious Disease Control, Karolinska Institute, Stockholm, Sweden
Antti Vaheri, Olli Vapalahti
Department of Virology, Haartman Institute, University of Helsinki, Helsinki, Finland
Paul K. S. Chan
Department of Microbiology, Faculty of Medicine, Chinese University of Hong Kong, Hong Kong, People's Republic of China
Heinz Feldmann, Daryl Dick
Special Pathogens Program, Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada, Canadian Science Centre for Human and Animal Health, Winnipeg, Manitoba, Canada
Jonas Schmidt-Chanasit
WHO Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and Research, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
Paula Padula
Departamento de Virología, Instituto Nacional de Enfermedades Infecciosas, A.N.L.I.S. ‘Dr. Carlos G. Malbrán,’ Buenos Aires, Argentina
Pablo A. Vial
Clínica Alemana School of Medicine, Institute of Science, Universidad del Desarrollo, Santiago, Chile
Raluca Panculescu-Gatej, Cornelia Ceianu
Laboratory of Vector-Borne Infections and Medical Entomology, CANTACUZINO National Institute for Research and Development in Microbiology and Immunology, Bucharest, Romania
Paul Heyman
Research Laboratory for Vector-Borne Diseases, Queen Astrid Military Hospital, Brussels, Belgium
Tatjana Avšič-Županc
Institute of Microbiology and Immunology, Laboratory for Diagnosis of Zoonoses and WHO Laboratory, Ljubljana, Slovenia
Matthias Niedrig
Center for Biological Safety (ZBS-1), Robert Koch Institute, Berlin, Germany

Corresponding Author

Email: e.lattwein@euroimmun.de

Competing Interests

I have read the journal's policy and have the following conflicts: S. Lederer and E. Lattwein are employees of EUROIMMUN AG. K. Sonnenberg and M. Hanke were employees of EUROIMMUN AG at the time of conducting this study or writing the manuscript, respectively. W. Stoecker is a board member of EUROIMMUN AG.

Author Contributions

Conceived and designed the experiments: KS SL WS MN. Performed the experiments: SL AV OV PKSC HF DD JSC PP PAV RPG CC. Analyzed the data: SL EL MH KS AV OV PKSC HF JSC PP PAV CC MN. Contributed reagents/materials/analysis tools: WS ÅL AV OV PKSC HF JSC PP PAV CC PH TAZ. Wrote the paper: MH EL SL WS AV PKSC HF JSC PP PAV CC PH TAZ MN.