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Table of Contents: July 2010

COVER

Image Credit: Emily Peak
Issue Image
The helminth fluorescent bioassay: An objective tool for quantifying schistosome viability.

Confocal microscopy of Schistosoma mansoni schistosomula illustrates the ease by which dead (propidium iodide-positive, red individuals) and live (fluorescein diacetate-positive, green individuals) parasites can be distinguished using a helminth fluorescent bioassay (HFB) described by Peak et al. (doi:10.1371/journal.pntd.0000759). It is anticipated that the HFB will be useful in objectively measuring schistosome survival during immunological assays (antibody- or complement-mediated killing), RNAi investigations, and drug screening protocols.

Editorial

Viewpoints

Mycobacterium ulcerans and Other Mycolactone-Producing Mycobacteria Should Be Considered a Single Species

Sacha J. Pidot, Kingsley Asiedu, Michael Käser, Janet A. M. Fyfe, Timothy P. Stinear

Research Articles

Neglected Tropical Diseases outside the Tropics

Francesca F. Norman, Ana Pérez de Ayala, José-Antonio Pérez-Molina, Begoña Monge-Maillo, Pilar Zamarrón, Rogelio López-Vélez

Rift Valley Fever Virus Seroprevalence in Human Rural Populations of Gabon

Xavier Pourrut, Dieudonné Nkoghé, Marc Souris, Christophe Paupy, Janusz Paweska, Cindy Padilla, Ghislain Moussavou, Eric M. Leroy

Phylogeography of Recently Emerged DENV-2 in Southern Viet Nam

Maia A. Rabaa, Vu Thi Ty Hang, Bridget Wills, Jeremy Farrar, Cameron P. Simmons, Edward C. Holmes

Randomised Controlled Double-Blind Non-Inferiority Trial of Two Antivenoms for Saw-Scaled or Carpet Viper (Echis ocellatus) Envenoming in Nigeria

Isa S. Abubakar, Saidu B. Abubakar, Abdulrazaq G. Habib, Abdulsalam Nasidi, Nandul Durfa, Peter O. Yusuf, Solomon Larnyang, John Garnvwa, Elijah Sokomba, Lateef Salako, R. David G Theakston, Ed Juszczak, Nicola Alder, David A. Warrell , for the Nigeria-UK EchiTab Study Group

Health Education through Analogies: Preparation of a Community for Clinical Trials of a Vaccine against Hookworm in an Endemic Area of Brazil

Maria Flavia Gazzinelli, Lucas Lobato, Leonardo Matoso, Renato Avila, Rita de Cassia Marques, Ami Shah Brown, Rodrigo Correa-Oliveira, Jeffrey M. Bethony, David J. Diemert

The Jacob2 Lectin of the Entamoeba histolytica Cyst Wall Binds Chitin and Is Polymorphic

Sudip K. Ghosh, Katrina L. Van Dellen, Anirban Chatterjee, Tuli Dey, Rashidul Haque, Phillips W. Robbins, John Samuelson

Single Nucleotide Polymorphism Typing of Mycobacterium ulcerans Reveals Focal Transmission of Buruli Ulcer in a Highly Endemic Region of Ghana

Katharina Röltgen, Weihong Qi, Marie-Thérèse Ruf, Ernestina Mensah-Quainoo, Sacha J. Pidot, Torsten Seemann, Timothy P. Stinear, Michael Käser, Dorothy Yeboah-Manu, Gerd Pluschke

High Rates of Homologous Recombination in the Mite Endosymbiont and Opportunistic Human Pathogen Orientia tsutsugamushi

Piengchan Sonthayanon, Sharon J. Peacock, Wirongrong Chierakul, Vanaporn Wuthiekanun, Stuart D. Blacksell, Mathew T. G. Holden, Stephen D. Bentley, Edward J. Feil, Nicholas P. J. Day

Evaluation of Chikungunya Diagnostic Assays: Differences in Sensitivity of Serology Assays in Two Independent Outbreaks

Grace Yap, Kwoon-Yong Pok, Yee-Ling Lai, Hapuarachchige-Chanditha Hapuarachchi, Angela Chow, Yee-Sin Leo, Li-Kiang Tan, Lee-Ching Ng

Comparing Diagnostic Accuracy of Kato-Katz, Koga Agar Plate, Ether-Concentration, and FLOTAC for Schistosoma mansoni and Soil-Transmitted Helminths

Dominik Glinz, Kigbafori D. Silué, Stefanie Knopp, Laurent K. Lohourignon, Kouassi P. Yao, Peter Steinmann, Laura Rinaldi, Giuseppe Cringoli, Eliézer K. N'Goran, Jürg Utzinger

A Tractable Experimental Model for Study of Human and Animal Scabies

Kate Mounsey, Mei-Fong Ho, Andrew Kelly, Charlene Willis, Cielo Pasay, David J. Kemp, James S. McCarthy, Katja Fischer

Emergence of the Asian 1 Genotype of Dengue Virus Serotype 2 in Viet Nam: In Vivo Fitness Advantage and Lineage Replacement in South-East Asia

Vu Thi Ty Hang, Edward C. Holmes, Duong Veasna, Nguyen Thien Quy, Tran Tinh Hien, Michael Quail, Carol Churcher, Julian Parkhill, Jane Cardosa, Jeremy Farrar, Bridget Wills, Niall J. Lennon, Bruce W. Birren, Philippe Buchy, Matthew R. Henn, Cameron P. Simmons

In Vitro and In Vivo High-Throughput Assays for the Testing of Anti-Trypanosoma cruzi Compounds

Adriana M. C. Canavaci, Juan M. Bustamante, Angel M. Padilla, Cecilia M. Perez Brandan, Laura J. Simpson, Dan Xu, Courtney L. Boehlke, Rick L. Tarleton

Farm-Level Risk Factors for Fish-Borne Zoonotic Trematode Infection in Integrated Small-Scale Fish Farms in Northern Vietnam

Van Thi Phan, Annette Kjær Ersbøll, Khue Viet Nguyen, Henry Madsen, Anders Dalsgaard

Kinetic Analysis of Ex Vivo Human Blood Infection by Leishmania

Inmaculada Moreno, Mercedes Domínguez, Darío Cabañes, Carmen Aizpurua, Alfredo Toraño

Integrated Surveys of Neglected Tropical Diseases in Southern Sudan: How Much Do They Cost and Can They Be Refined?

Jan H. Kolaczinski, Kara Hanson, Emily Robinson, Diana Picon, Anthony Sabasio, Martin Mpakateni, Mounir Lado, Stephen Moore, Nora Petty, Simon Brooker

Family Relationship, Water Contact and Occurrence of Buruli Ulcer in Benin

Ghislain Emmanuel Sopoh, Yves Thierry Barogui, Roch Christian Johnson, Ange Dodji Dossou, Michel Makoutodé, Sévérin Y. Anagonou, Luc Kestens, Françoise Portaels

Dengue Dynamics in Binh Thuan Province, Southern Vietnam: Periodicity, Synchronicity and Climate Variability

Khoa T. D. Thai, Bernard Cazelles, Nam Van Nguyen, Long Thi Vo, Maciej F. Boni, Jeremy Farrar, Cameron P. Simmons, H. Rogier van Doorn, Peter J. de Vries

Antiangiogenic and Antitumor Effects of Trypanosoma cruzi Calreticulin

Nandy C. López, Carolina Valck, Galia Ramírez, Margarita Rodríguez, Carolina Ribeiro, Juana Orellana, Ismael Maldonado, Adriana Albini, Daniel Anacona, David Lemus, Lorena Aguilar, Wilhelm Schwaeble, Arturo Ferreira

Seasonal and Regional Dynamics of M. ulcerans Transmission in Environmental Context: Deciphering the Role of Water Bugs as Hosts and Vectors

Estelle Marion, Sara Eyangoh, Edouard Yeramian, Julien Doannio, Jordi Landier, Jacques Aubry, Arnaud Fontanet, Christophe Rogier, Viviane Cassisa, Jane Cottin, Agnès Marot, Matthieu Eveillard, Yannick Kamdem, Pierre Legras, Caroline Deshayes, Jean-Paul Saint-André, Laurent Marsollier

Serological Studies of Neurologic Helminthic Infections in Rural Areas of Southwest Cameroon: Toxocariasis, Cysticercosis and Paragonimiasis

Agathe Nkouawa, Yasuhito Sako, Sonoyo Itoh, Alida Kouojip-Mabou, Christ Nadège Nganou, Yasuaki Saijo, Jenny Knapp, Hiroshi Yamasaki, Minoru Nakao, Kazuhiro Nakaya, Roger Moyou-Somo, Akira Ito

Where Do We Go from Here? Prevalence of Trachoma Three Years after Stopping Mass Distribution of Antibiotics in the Regions of Kayes and Koulikoro, Mali

Sanoussi Bamani, Jonathan D. King, Mamadou Dembele, Famolo Coulibaly, Dieudonne Sankara, Yaya Kamissoko, Jim Ting, Lisa A. Rotondo, Paul M. Emerson

Response to Treatment in a Prospective Cohort of Patients with Large Ulcerated Lesions Suspected to Be Buruli Ulcer (Mycobacterium ulcerans Disease)

Kapay Kibadi, Marleen Boelaert, Alexandra G. Fraga, Makanzu Kayinua, Adhemar Longatto-Filho, Jean-Bedel Minuku, Jean-Baptiste Mputu-Yamba, Jean-Jacques Muyembe-Tamfum, Jorge Pedrosa, Jean-Jacques Roux, Wayne M. Meyers, Françoise Portaels

Phase II Evaluation of Sensitivity and Specificity of PCR and NASBA Followed by Oligochromatography for Diagnosis of Human African Trypanosomiasis in Clinical Samples from D.R. Congo and Uganda

Enock Matovu, Claire M. Mugasa, Rosine Ali Ekangu, Stijn Deborggraeve, George W. Lubega, Thierry Laurent, Gerard J. Schoone, Henk D. Schallig, Philippe Büscher

Comparison of Three Commercially Available Dengue NS1 Antigen Capture Assays for Acute Diagnosis of Dengue in Brazil

Monique da Rocha Queiroz Lima, Rita Maria Ribeiro Nogueira, Hermann Gonçalves Schatzmayr, Flavia Barreto dos Santos