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Public Library of Science

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Issue Image

PLoS Neglected Tropical Diseases Issue Image | Vol. 4(7) July 2010

<bold>The helminth fluorescent bioassay: An objective tool for quantifying schistosome viability.</bold>

Confocal microscopy of Schistosoma mansoni schistosomula illustrates the ease by which dead (propidium iodide-positive, red individuals) and live (fluorescein diacetate-positive, green individuals) parasites can be distinguished using a helminth fluorescent bioassay (HFB) described by Peak et al. (doi:10.1371/journal.pntd.0000759). It is anticipated that the HFB will be useful in objectively measuring schistosome survival during immunological assays (antibody- or complement-mediated killing), RNAi investigations, and drug screening protocols.

Image Credit: Emily Peak

The helminth fluorescent bioassay: An objective tool for quantifying schistosome viability.  Top

Confocal microscopy of Schistosoma mansoni schistosomula illustrates the ease by which dead (propidium iodide-positive, red individuals) and live (fluorescein diacetate-positive, green individuals) parasites can be distinguished using a helminth fluorescent bioassay (HFB) described by Peak et al. (doi:10.1371/journal.pntd.0000759). It is anticipated that the HFB will be useful in objectively measuring schistosome survival during immunological assays (antibody- or complement-mediated killing), RNAi investigations, and drug screening protocols.

Image Credit: Emily Peak

Citation: (2010) PLoS Neglected Tropical Diseases Issue Image | Vol. 4(7) July 2010. PLoS Negl Trop Dis 4(7): ev04.i07. doi:10.1371/image.pntd.v04.i07

Published: July 27, 2010

Copyright: © 2010 Emily Peak. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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The helminth fluorescent bioassay: An objective tool for quantifying schistosome viability.

Confocal microscopy of Schistosoma mansoni schistosomula illustrates the ease by which dead (propidium iodide-positive, red individuals) and live (fluorescein diacetate-positive, green individuals) parasites can be distinguished using a helminth fluorescent bioassay (HFB) described by Peak et al. (doi:10.1371/journal.pntd.0000759). It is anticipated that the HFB will be useful in objectively measuring schistosome survival during immunological assays (antibody- or complement-mediated killing), RNAi investigations, and drug screening protocols.

Image Credit: Emily Peak

doi:10.1371/image.pntd.v04.i07.g001
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