Advertisement
Research Article

Identification of Tsetse (Glossina spp.) Using Matrix-Assisted Laser Desorption/Ionisation Time of Flight Mass Spectrometry

  • Antje Hoppenheit mail,

    antje.hoppenheit@fu-berlin.de (AH); jayaseelan.murugaiyan@fu-berlin.de (JM)

    Affiliation: Freie Universität Berlin, Zentrum für Infektionsmedizin, Institute for Parasitology and Tropical Veterinary Medicine, Berlin, Germany

    Considered as first authors.

    X
  • Jayaseelan Murugaiyan mail,

    antje.hoppenheit@fu-berlin.de (AH); jayaseelan.murugaiyan@fu-berlin.de (JM)

    Affiliation: Freie Universität Berlin, Zentrum für Infektionsmedizin, Institute of Animal Health and Environmental Hygiene, Berlin, Germany

    Considered as first authors.

    X
  • Burkhard Bauer,

    Affiliation: Freie Universität Berlin, Zentrum für Infektionsmedizin, Institute for Parasitology and Tropical Veterinary Medicine, Berlin, Germany

    X
  • Stephan Steuber,

    Affiliation: Federal Office of Consumer Protection and Food Safety (BVL), Berlin, Germany

    X
  • Peter-Henning Clausen,

    Affiliation: Freie Universität Berlin, Zentrum für Infektionsmedizin, Institute for Parasitology and Tropical Veterinary Medicine, Berlin, Germany

    X
  • Uwe Roesler

    Affiliation: Freie Universität Berlin, Zentrum für Infektionsmedizin, Institute of Animal Health and Environmental Hygiene, Berlin, Germany

    X
  • Published: July 11, 2013
  • DOI: 10.1371/journal.pntd.0002305

Abstract

Glossina (G.) spp. (Diptera: Glossinidae), known as tsetse flies, are vectors of African trypanosomes that cause sleeping sickness in humans and nagana in domestic livestock. Knowledge on tsetse distribution and accurate species identification help identify potential vector intervention sites. Morphological species identification of tsetse is challenging and sometimes not accurate. The matrix-assisted laser desorption/ionisation time of flight mass spectrometry (MALDI TOF MS) technique, already standardised for microbial identification, could become a standard method for tsetse fly diagnostics. Therefore, a unique spectra reference database was created for five lab-reared species of riverine-, savannah- and forest- type tsetse flies and incorporated with the commercial Biotyper 3.0 database. The standard formic acid/acetonitrile extraction of male and female whole insects and their body parts (head, thorax, abdomen, wings and legs) was used to obtain the flies' proteins. The computed composite correlation index and cluster analysis revealed the suitability of any tsetse body part for a rapid taxonomical identification. Phyloproteomic analysis revealed that the peak patterns of G. brevipalpis differed greatly from the other tsetse. This outcome was comparable to previous theories that they might be considered as a sister group to other tsetse spp. Freshly extracted samples were found to be matched at the species level. However, sex differentiation proved to be less reliable. Similarly processed samples of the common house fly Musca domestica (Diptera: Muscidae; strain: Lei) did not yield any match with the tsetse reference database. The inclusion of additional strains of morphologically defined wild caught flies of known origin and the availability of large-scale mass spectrometry data could facilitate rapid tsetse species identification in the future.

Author Summary

Tsetse flies are confined to tropical Africa and are carriers for trypanosomes, single-celled blood parasites. Through the bite of an infective tsetse, people and animals may contract trypanosomiasis, a degenerative disease leading to death if left untreated. Tsetse control proved effective for disease containment, but data on the flies as tsetse identification are a prerequisite for planning any control intervention. There are 32 generally accepted tsetse species and subspecies. Classical species identification relies on minor morphological differences, often challenging for field workers. In the last decade, Matrix-Assisted Laser Desorption/Ionisation (MALDI) has revolutionised microbial species identification. After a simple protein extraction, a laser-induced ionisation takes place. Then, the ions are accelerated in a vacuum tube, and their Time of Flight (ToF) to reach the detector is recorded. The protein composition of each organism is unique, and so is their MALDI signature. Comparison of the obtained signature with a database of known organisms enables rapid identification as reliable as genome-based methods. To possibly speed up tsetse diagnostics, we established a MALDI database for the identification of five defined laboratory tsetse breeds. Inclusion of wild-caught tsetse could reinforce the reference database for the identification of tsetse at the species and subspecies level.

Introduction

The trypanosomiasis infection risk of a particular area is determined by several factors, including tsetse species abundance and the sex distribution of a fly population [1]. While the sex is easily distinguishable with the bare eye, species identification can be challenging because there are 32 recognised tsetse species and subspecies [2]. Differentiation relies on morphological differences in colour, size and on minimal male genitalia variations [3]. Recent genome-based analyses revealed the subspecies status of seemingly uniform riverine G. palpalis palpalis individuals in Equatorial Guinea [4]. Accordingly, current tsetse specification based on morphology may not be the only way to rapidly determine the species status of Glossina spp.

The matrix assisted laser desorption/ionisation time of flight mass spectrometry (MALDI-TOF MS) is an established method of identification for microorganisms [5], [6], [7], [8], [9], [10], [11]. The MALDI-based identification of microorganisms requires only a small portion of a microbial colony and a drop of matrix solution [12], [13], [14]. The intact microbial cells are mixed with matrix solution (UV observing substances like alpha-Cyano-4-hydroxycinnamic acid, 2,5-dihydroxybenzoic acid), dried and subjected to laser induced soft ionization. The ions are then accelerated into a vacuum tube using a high electric field and the Time of Flight (ToF) to reach the detector is recorded. The velocity of an ion is inversely proportional to its mass, thus smaller ions travel faster than heavier ones and ions with the same charge travel together. The ions hitting the detector and their time of flight are visualized as spectra. The protein composition of each organism is unique, so a species-specific MALDI signature or spectrum is expected. The species identification does not require protein sequence data; instead the acquired spectra are matched with reference spectra database using a pattern- matching algorithm [9], [11]. The technique proved to be time and cost effective, as reliable as genome-based identification methods [6], [9]. Recently, MALDI-based species identification has been demonstrated for higher organisms as micro-algae, Prototheca [15], [16], the plant parasitic nematode Anguina tritici [17], Drosophila [18], [19], ticks [20] biting midges (Culicoides spp.) [21], [22], [23] and fish [24]. In addition MALDI has also been utilised for differentiation of various eukaryotic cell lines [25], immune cells [26], [27] and for species level classification of ancient mammalian samples [28].

Several commercial software packages designed for microbial species identification are available and include, MALDI Biotyper (Bruker Daltonics), the Axima (Shimadzu)-SARAMIS (AnagnosTec) systems (now called VITEK MS) (BioMérieux), Andromas (Andromas SAS) systems and MicrobeLynx (Waters) [7], [8], [29]. As far as our knowledge is concerned, reference spectra data for insects or tsetse in particular have not been included in any of these software packages. We chose the MALDI Biotyper system for creating a tsetse-specific spectra database. This system calculates the log score value, or similarity score, by considering the matching proportion of the test spectra with the database reference spectra. It also considers the consistency of peak intensities among sample and reference spectra.

The objective of this study was to investigate whether simple formic acid/acetonitrile extracts of five well known laboratory-reared tsetse breeds exhibit specific and reproducible peak patterns and if they prove to be valid for species level identification. Usually, field-collected tsetse are stored in ethanol and often parts of the insects are removed for diagnostics. Therefore, another goal was to investigate if any of the body parts (head, thorax, abdomen, legs, wings and whole insects) are useful for species prediction.

Materials and Methods

Tsetse selection and storage

To establish a tsetse database, we utilised five well-established laboratory breeds listed in table 1. They represent tsetse from three different habitats that are relevant for the transmission of trypanosomes that affect humans or animals [2]. Tsetse puparia were maintained at 26°C with a relative humidity of 75%. Two to 4 days after hatching they were sacrificed as tenerals at −18°C and then stored in ethanol (70%).

thumbnail

Table 1. Laboratory-reared Glossina (G.) spp. selected for the compilation of spectra database.

doi:10.1371/journal.pntd.0002305.t001

Fly dissection, protein extraction and MALDI measurement

A total of three insects each were obtained for the analysis of male and female entire individuals (table 1). Additionally, three males and females of each species were dissected representing the peak patterns of the body parts abdomen, head, legs, thorax and wings. The protein extraction was carried out as described in Murugaiyan et al. [16]. In brief, triplicates of each specimen (whole insect, head, thorax, abdomen, wings and legs) were washed with ethanol, air dried and mixed with equal volumes of 70% formic acid and 100% acetonitrile. The samples were then sonicated for 1 min on ice and the supernatants were collected for further analysis. One µl of each sample extract was spotted on to the MALDI target plate (MSP 96 target polished steel (MicroScout Target) plate Bruker Daltonics, Bremen, Germany), dried and overlaid with 1.0 µl of saturated α-cyano-4-hydroxycinnamic acid matrix solution. The MALDI measurements were carried out using MALDI Microflex LT (Bruker Daltonics, Bremen, Germany) on a broad range of 2000–20000 m/z (mass to charge ratio), following an external calibration with the bacterial test standard as recommended by the manufacturer. Each extract was spotted three times and each spot on the target plate was measured three times for acquiring 27 spectra per specimen. The spectra were acquired using the automated option (AutoXecute acquisition mode) in Flex control 3.0 software (Bruker Daltonics, Leipzig, Germany). (Box 1)

Box 1. Key Steps in Maldi Microbial Identification and the Software Used in This Study

With the aim of creating a simple protein extraction and identification procedure for tsetse, we utilized the well-established microbial method of MALDI identification:

  1. Protein extraction
  2. Spot on target plate, overlaid with matrix solution and dried
  3. Spectra acquisition
  4. Peak picking and pattern matching with the database

The protein extraction is an essential step for creating reference spectra of multicellular organisms while direct transfer of microbial colonies from the culture plate is sufficient for microbial identification. In this study commercial software associated with MALDI MicroFlex LT (Bruker Daltonics, Bremen, Germany) are utilized to create a tsetse specific database that draws from individual mass spectrum peaks. Spectra acquisition is carried out by the software Flexcontrol 3.0 and selection is performed manually after visualisation using FlexAnalysis 3.0. software. Final tsetse reference spectra were created with the software Biotyper 3.0 that includes the manufacturer's reference database.

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)

In order to demonstrate the protein composition in each extract, Glossina (G.) palpalis gambiensis were chosen for an SDS-PAGE analysis [30]. In brief, the extracts of the whole insects and it's the body parts were precipitated in five volumes of ice-cold 100% acetone. The pellets were reconstituted with 10 µl of sample loading buffer, heated at 60°C for 5 minutes and separated using 4% stacking and 12% separating gel. The protein visualisation was carried out using Coomassie Blue staining [31].

Data analysis and creation of tsetse reference spectra

Following the visual inspection using Flex analysis 3.0 software (Bruker Daltonics, Bremen, Germany), the spectra were then loaded in Biotyper 3.0 (Bruker Daltonics, Bremen, Germany) software. The spectra were subjected to baseline subtraction (multipolygonal; signal to noise ratio 3) and smoothing (Savitzky Golay algorithm, frame size 25 Da). The composite correlation index [32], a mathematical algorithm used to assess the spectra variations within and between each set of the measurements. The Composite Correlation Index (CCI) was computed using the standard settings of mass range 3000–12000 Da, resolution 4, four intervals and autocorrelation off. The reference spectra were then created using the standard method version 1.2 settings of the software (mass error of each single spectra: 2000, desired mass error of main spectra: 200, peak frequency: 25% and desired peak number: 70). The cluster analysis (main spectra dendrogram) was calculated with “correlation” as distance measure and linkage at “complete” to evaluate the suitability of the MALDI-based differentiation of tsetse at the species level. The created main spectra were then compiled as a tsetse database.

Evaluation of the tsetse database

In order to check the suitability of the created tsetse main spectra for Biotyper-based species identification, the cross-matching status was created after matching them to the entire database. In addition, fresh extractions of the whole insect and the various insect parts were utilized in triplicates to cross-check the efficiency of the established tsetse database. For ruling out possible cross-matching with other fly species, the common house fly Musca domestica (Diptera: Muscidae; strain Lei) was also included in the evaluation. Identification was carried out using the Biotyper 3.0 software tool, following the manufacturer's recommendation on identification based on the calculated log score values. Values of ≥2.0 to 3.0 represent probable species level matching, while scores of ≥1.7 to 1.9 represent probable genus level matching. A score value of <1.7 stands for an unreliable identification.

Results

From each tsetse specimen a total of 27 spectra representing biological and technical replicates in the m/z range of 2000–20000 Da were acquired automatically and thus 1620 spectra from whole Glossina species and their body parts A–J. Visual inspection of the spectra revealed a comparable peak pattern of the biological and technical replicates; however, differences in peak intensities were observed for example as shown in figure 1.

thumbnail

Figure 1. Spectra reproducibility among the biological and technical replicates.

Overlay view of 27 spectra obtained from biological and technical replicates of Glossina austeni female whole insect. The masses (in Da) of the ions are shown on the x-axis and the m/z value stands for mass to charge ratio. On the y-axis, the relative intensity of the ions (a.u., arbitrary units) is shown. In the insert, zoomed m/z 5000 to 5200 displays the uniformity among the measured spectra and the stacked view m/z 9000 to 12500 provides a direct comparison of all 27 measured spectra.

doi:10.1371/journal.pntd.0002305.g001

At first look, the raw spectrum displayed consistently distinct peak patterns when comparing the two sexes of G. palpalis gambiensis (figure 2, samples G/H at m/z 5700, 7000 and 8000) while the three savannah species (A–F) and G. brevipalpis (I/J) only displayed differences in peak intensity. Occasionally observed differences as seen in the G. pallidipes female (sample E at 8100 m/z) appeared inconsistently. However, several peaks showed to be common for Glossina spp. as for instance presented in figure 2 at 5000 m/z.

thumbnail

Figure 2. Representative spectra of whole insect extraction of male and female Glossina spp.

Mass spectra peak pattern of whole insect extractions of male and female Glossina (G.) spp. The x-axis m/z value stands for mass to charge ratio and the relative intensity of the ions (a.u., arbitrary units) is shown on the y-axis. A) G. morsitans morsitans female, B) G. morsitans morsitans male, C) G. austeni female, D) G. austeni male, E) G. pallidipes female F) G. pallidipes male, G) G. palpalis gambiensis female, H) G. palpalis gambiensis male, I) G. brevipalpis female, and J) G. brevipalpis male.

doi:10.1371/journal.pntd.0002305.g002

As shown in figure 3, the raw spectra of different body parts and the entire insects presented varying peak patterns at least in terms of peak intensities. Among the body parts, peak intensities sometimes tended to be lower in some of the leg extracts when compared to entire insects or other parts. To demonstrate the protein composition of whole insects and the different body parts, G. palpalis gambiensis extracts were chosen for protein separation on SDS-PAGE and visualised using a modified Coomassie staining. As shown in figure 4, the protein separation was carried out from 10 to 200 kDa. The bands out of the extracts of the dissected body parts were clearly observed in the whole insect protein extract lane. However, it should be noted that the peaks in the MALDI spectra were obtained from much smaller peptides (2–20 kDa).

thumbnail

Figure 3. Representative spectra from the whole insect and different body parts of female Glossina austeni.

Peak pattern of whole and body parts extractions of Glossina austeni female. The x-axis m/z values represent the mass to charge ratio and on the y-axis the relative intensity of the ions (a.u., arbitrary units) is shown. A) Whole insect, B) abdomen, C) head, D) legs, E) thorax and F) wings.

doi:10.1371/journal.pntd.0002305.g003
thumbnail

Figure 4. SDS PAGE separation of Glossina palpalis gambiensis protein extracts.

SDS-PAGE separation of the whole insect and bodyparts of Glossina palpalis gambiensis extracts. 10 µg of proteins were run on 12% SDS-PAGE and stained with Coomassie brilliant blue.

doi:10.1371/journal.pntd.0002305.g004

Figure 5 depicts the colour-coded computed composite correlation index [31] displaying the uniqueness of the acquired spectra 1–60. A CCI value of 0.0 (dark green) represents incongruency and 1.0 (red) denotes complete congruency. The CCI was observed between 0.68 and 0.98 (individual CCI values are shown in the supplement data table S1). Very few of the spectra sets displayed some deviation among themselves, for e.g. the CCI for G. austeni male head was 0.68. However, this spectra set displayed a complete deviation with other body parts or other species. Despite this shortcoming, the spectra sets appeared to be suitable for the compilation of a reference spectra library.

thumbnail

Figure 5. Composite correlation index of tsetse spectra sets.

Evaluation of uniqueness among the spectra sets of 60 tsetse spectra measurements of male (M) and female (F) individuals and their body parts. Composite correlation index matrix was calculated with Biotyper 3.0 software in the mass range of 3000–12000 Da, resolution 4, 4 intervals and auto-correction off. Red indicates relatedness between the spectra sets and dark green indicates incongruence.

doi:10.1371/journal.pntd.0002305.g005

Cross–comparison of the tsetse main spectra with the entire Bruker reference database resulted in only one clear match with a log score value of >2.3, the cut-off value representing the most probable matching at the species-level. Some isolates such as G. austeni female head (no. 2) appeared to resemble G. palpalis gambiensis male head spectra (no. 56), however, the score value was distinctly lower than the expected matching set. This clearly indicated that these spectra sets could be utilized to establish a database.

Following these preliminary investigations, the main spectra library representing the 70 most reproducible peaks was constructed. The cluster analysis of the 10 main spectra of each species is shown in figure 6 for both sexes. Consistent clustering was observed among the extracts of G. brevipalpis, which always stood out as a sister group to the other species regardless of the body part. Furthermore, G. austeni showed inconsistent clustering, neither similar to savannah group tsetse nor to riverine G. palpalis gambiensis as for instance seen in the dendrogram.

thumbnail

Figure 6. Score-oriented main spectra dendrogram of whole Glossina spp. extracts.

The dendrogram was calculated by Biotyper 3.0 software with distance measure set at correlation and linkage set at complete.

doi:10.1371/journal.pntd.0002305.g006

The created tsetse main spectra were incorporated into the commercial Bruker system and then compared with the whole database following the manufacturer's recommendation. Accordingly, table S2 of the supplementary data describes the matching of tsetse main spectra where log score value 3.0 stands for a 100% match and lower matching probabilities were displayed as subsequent hits. The results indicate that the second hit within the acceptable cut-off value of >2.0 for some of the body part extracts matched with the correct body part but irrespective of the factors sex and species. This cross matching of body parts was predominantly observed between G. austeni and G. morsitans morsitans and among G. pallidipes and G. palpalis gambiensis. Within the same species, complete deviation was observed in G. austeni female head with its own abdomen and legs, Similarly, G. palpalis gambiensis female head did not match with its legs and thorax. G. palpalis gambiensis male head also displayed complete deviation with G. palpalis gambiensis female head.

As shown in table 2 (detailed identification results are listed in supplementary table S3), the results of fresh sample identification clearly indicate that every body part and sex was correctly matched at the species level (log score value >2.0). Despite the 100% correct identification, within this high confident identification the following score inconsistencies occurred: 58% (35/60) matched with the correct body part but also with the ones of the opposite sex, 35% (21/60) matched with the correct sex but with different body parts, 16% (10/60) matched with a different body part and the opposite sex and 5% (3/60) even matched with other species. The second best matching hits indicate that about 23% (14/60) of body parts displayed lower cut-off values (log score <1.7). Among the second best hits, incorrect matching was observed among 13 samples (21%): body parts of female G. palpalis gambiensis (thorax, whole and abdomen), G. pallidipes (female thorax) and G.austeni (male legs). The extracts from Musca domestica resulted in no reliable identification.

thumbnail

Table 2. Matching of five lab-reared tsetse with the Bruker reference database.

doi:10.1371/journal.pntd.0002305.t002

Discussion

To establish a tsetse reference database five laboratory breeds representing epidemiologically important tsetse of the savannah type G. morsitans morsitans, G. pallidipes and G. austeni, a riverine type G. palpalis gambiensis and forest type G. brevipalpis were chosen for this study [2], [33], [34] . Earlier attempts on the identification of arthropods by MALDI were carried out after homogenisation of the samples and extraction in a mass spectrometry-compatible buffer system [18], [20], [21]. We used a standard formic acid/acetonitrile extraction procedure of microbial cell processing for the protein extraction from tsetse. We introduced an additional step of sonication in order to facilitate the breakage of the chitin shell for a better protein yield. This simple extraction method was chosen to accommodate the field-collected samples that are stored in ethanol and possibly dissected.

Flex analysis software revealed that the spectra of the same species appeared to be fairly comparable despite the varying peak intensities. Visual inspection of the spectra revealed differences among the body parts of the same insect. Often, the most intense peaks of body part extracts were not easily observable in the spectra of whole insect extracts. This could be due to the protein ionisation influenced by varying protein compositions/abundances of different body part extracts. Additional evaluation of the protein composition/abundance using SDS-PAGE protein separation revealed the difference in protein bands. However, the bands of the body part extracts were comparable to those of the whole insect but they varied in their intensities. This was also shown among the different sexes of the same species. As the protein separation was carried out in a higher range (10 to 200 kDa) but the MALDI spectra stemmed from a much smaller range of proteins (2 to 20 kDa), So, a direct correlation among these could not be expected. However, the compositional protein differences among the various body part extracts and the whole insect are clear. This protein compositional difference might attribute to the observed difference among the spectra from different insect body parts. Despite this variation, the technical and biological replicates appeared to influence the peak intensity while the peak pattern was almost comparable.

Among the commercially available software tools for species identification, we used Biotyper software that incorporates 4613 main reference spectra of microbial species (March 2013). The software automatically pre-process the spectra through smoothing and baseline subtraction. The peaks were picked and compared with the reference database. The results were expressed as similarity log score values between 3.0 (complete matching) to 0 (complete deviation). As a first step of the main spectra creation, the practical relation among the spectra sets was visualised by computing the composite correlation index [32]. A CCI value approaching 1 is considered to be highly significant while zero represents complete deviation. A clear distinction between the spectra sets of different body parts and the whole insect extracts was displayed in the heat-map and its corresponding value. Some of the spectra set displayed signs of deviation, for e.g. G. austeni male head. This might be due to the presence of broader peaks, which did not overlap with the corresponding spectra [32]. The heat-map and CCI values indicated that the spectra sets of different body parts and the whole insect extracts were unique and could be utilised for the creation of a spectra library. Therefore, we generated 60 main spectra for five tsetse species including male and female whole insect extracts and the corresponding body parts. These main spectra were then incorporated in the Bruker database.

The main spectra dendrogram was useful for the differentiation of the five species, picturing the similarities and differences of their mass spectra profiles. Clustering of the created tsetse main spectra revealed that they did not follow any distinct pattern with some significant exceptions. A possible explanation could be that higher organisms like insects might not cluster at the species level using MALDI measurements unless they are being standardised. However, G. austeni never clustered clearly with riverine nor savannah species; it seems to share mass spectra patterns with both groups reflecting the uncertainty of their phylogenetic status [35]. Very clearly though was the uniqueness of G. brevipalpis compared to the other species. The sister status deriving from genomic findings [36] could therefore be mirrored in the mass spectrum peaks of G. brevipalpis.

As a quality check, tsetse main spectra were cross-identified with the entire database from the manufacturer. All the tsetse main spectra matched with a log score of 3.0, indicating a clear distinction between the species. It also showed the uniqueness of the tsetse mass spectra for entire tsetse as well as every dissected body part. Among the second best matched hits, sex and species appeared to be least important while the body parts across the species matched, especially among G. austeni and G. morsitans morsitans and also in G. pallidipes and G. palpalis gambiensis extracts. The complete deviation of head extracts (G. austeni female, G. palpalis gambiensis female and male) indicates special attention when working on species identification of head samples by MALDI.

The fresh protein extracts using the same insects resulted in 100% matches with the database. No hits were achieved for similarly processed Musca domestica extracts, indicating the uniqueness of the created reference spectra for tsetse. Among the best hits at the species level, body parts of the same species appeared to be matched correctly but irrespective of the sex. A deviating species in the second hit might be due to the presence of shared metabolic proteins among different tsetse species. The 5% that mismatched completely and the incorrect matching among the second hits indicate that the reference database should be created for more than one body part and of both sexes for reliable identification of insects.

The overall results clearly indicate that the success in MALDI-based identification relies on the specific signature from the body parts and the whole insects. While the first hit for these lab breed tsetse appeared to be specific for species, sex and body parts, the second hit indicates that sex is the least reliable feature of MALDI identification. The complete deviation of head extracts with its own other body parts as seen among G. austeni and G. palpalis gambiensis indicate that more than one body part is needed for accurate species identification. We propose the addition of spectra from field-caught tsetse (whole insects and body parts) to extend our database for a fast and accurate identification of tsetse.

Supporting Information

Table S1.

Composite Correlation Index (CCI) values of 60 spectra sets of tsetse. CCI was calculated using Biotyper 3.0 software (Bruker Daltonics, Bremen, Germany) in the mass range of 3000–12000 Da, resolution 4, 4 intervals and auto-correction off. CCI value nearing 1.0 indicates the relatedness between the spectral set and 0 indicates deviation among the spectra sets. M-male and F-female.

doi:10.1371/journal.pntd.0002305.s001

(XLSX)

Table S2.

Cross matching values of tsetse main spectra. The created tsetse main spectra were selected in Biotyper 3.0 (Bruker Daltonics, Bremen, Germany) software and matched with the entire database. The log score value 3.0 indicates complete matching and 0 represents complete deviation. The manufacturer's recommended log score values, ≥2.0 to 3.0, ≥1.7 to 1.9 and <1.7 were utilised to interpret the identification as probable species level, genus level and no reliable identification respectively.

doi:10.1371/journal.pntd.0002305.s002

(XLSX)

Table S3.

Identification results of freshly extracted tsetse samples. The insect proteins from whole insects and its body parts were extracted using formic acid/acetonitrile. 1.0 µl of the extracted was spotted on the target plate, air dried, 1.0 µl of saturated HCCA matrix was overlaid and dried completely. The result interpretation was carried out in accordance to the manufacturer's recommended cutoff log score values for species (≥2.0 to 3.0), genus (≥1.7 to 1.9) and the value lesser than 1.69 indicated that the samples were not reliable matched with any of the reference spectra.

doi:10.1371/journal.pntd.0002305.s003

(XLSX)

Acknowledgments

We would like to thank the International Atomic Energy Agency in Seibersdorf, Austria, for providing us with tsetse puparia and Dr. Marta Maia who assisted us in obtaining puparia from Tanga, Tanzania. We would also like to place our sincere thanks to Bettina Esch for her excellent technical support.

Author Contributions

Conceived and designed the experiments: PHC UR AH JM BB SS. Performed the experiments: AH JM. Analyzed the data: AH JM. Contributed reagents/materials/analysis tools: UR PHC. Wrote the paper: AH JM.

References

  1. 1. Rogers DJ, Hendrickx G, Slingenbergh JH (1994) Tsetse flies and their control. Rev Sci Tech 13: 1075–1124.
  2. 2. Leak SGA (1998) Tsetse Biology and Ecology: Their role in the Epidemiology and Control of Trypanosomiasis. New York: CABI Publishing. 592 p.
  3. 3. FAO (1992) Tsetse biology, systematics and distribution, techniques. Training Manual for Tsetse Control Personnel. Rome: Food and Agriculture Organization of the United Nations. 280 p.
  4. 4. Dyer NA, Furtado A, Cano J, Ferreira F, Odete Afonso M, et al. (2009) Evidence for a discrete evolutionary lineage within Equatorial Guinea suggests that the tsetse fly Glossina palpalis palpalis exists as a species complex. Mol Ecol 18: 3268–3282. doi: 10.1111/j.1365-294x.2009.04265.x
  5. 5. Claydon MA, Davey SN, Edwards-Jones V, Gordon DB (1996) The rapid identification of intact microorganisms using mass spectrometry. Nat Biotechnol 14: 1584–1586. doi: 10.1038/nbt1196-1584
  6. 6. Welker M (2011) Proteomics for routine identification of microorganisms. Proteomics 11: 3143–3153. doi: 10.1002/pmic.201100049
  7. 7. Bille E, Dauphin B, Leto J, Bougnoux ME, Beretti JL, et al. (2012) MALDI-TOF MS Andromas strategy for the routine identification of bacteria, mycobacteria, yeasts, Aspergillus spp. and positive blood cultures. Clin Microbiol Infect 18: 1117–1125. doi: 10.1111/j.1469-0691.2011.03688.x
  8. 8. van Belkum A, Welker M, Erhard M, Chatellier S (2012) Biomedical mass spectrometry in today's and tomorrow's clinical microbiology laboratories. J Clin Microbiol 50: 1513–1517. doi: 10.1128/jcm.00420-12
  9. 9. Kliem M, Sauer S (2012) The essence on mass spectrometry based microbial diagnostics. Curr Opin Microbiol 15: 397–402. doi: 10.1016/j.mib.2012.02.006
  10. 10. Sauer S, Freiwald A, Maier T, Kube M, Reinhardt R, et al. (2008) Classification and identification of bacteria by mass spectrometry and computational analysis. PLoS One 3: e2843. doi: 10.1371/journal.pone.0002843
  11. 11. Croxatto A, Prod'hom G, Greub G (2012) Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology. FEMS Microbiol Rev 36: 380–407. doi: 10.1111/j.1574-6976.2011.00298.x
  12. 12. Demirev PA, Fenselau C (2008) Mass spectrometry for rapid characterization of microorganisms. Annu Rev Anal Chem (Palo Alto Calif) 1: 71–93.
  13. 13. Sedo O, Sedlacek I, Zdrahal Z (2011) Sample preparation methods for MALDI-MS profiling of bacteria. Mass Spectrom Rev 30: 417–434. doi: 10.1002/mas.20287
  14. 14. Toh-Boyo GM, Wulff SS, Basile F (2012) Comparison of sample preparation methods and evaluation of intra- and intersample reproducibility in bacteria MALDI-MS profiling. Anal Chem 84: 9971–9980. doi: 10.1021/ac302375e
  15. 15. von Bergen M, Eidner A, Schmidt F, Murugaiyan J, Wirth H, et al. (2009) Identification of harmless and pathogenic algae of the genus Prototheca by MALDI-MS. Proteomics Clin Appl 3: 774–784. doi: 10.1002/prca.200780138
  16. 16. Murugaiyan J, Ahrholdt J, Kowbel V, Roesler U (2012) Establishment of a matrix-assisted laser desorption ionization time-of-flight mass spectrometry database for rapid identification of infectious achlorophyllous green micro-algae of the genus Prototheca. Clin Microbiol Infect 18: 461–467. doi: 10.1111/j.1469-0691.2011.03593.x
  17. 17. Perera MR, Vanstone VA, Jones MG (2005) A novel approach to identify plant parasitic nematodes using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 19: 1454–1460. doi: 10.1002/rcm.1943
  18. 18. Feltens R, Gorner R, Kalkhof S, Groger-Arndt H, von Bergen M (2010) Discrimination of different species from the genus Drosophila by intact protein profiling using matrix-assisted laser desorption ionization mass spectrometry. BMC Evol Biol 10: 95. doi: 10.1186/1471-2148-10-95
  19. 19. Campbell PM, Harcourt RL, Crone EJ, Claudianos C, Hammock BD, et al. (2001) Identification of a juvenile hormone esterase gene by matching its peptide mass fingerprint with a sequence from the Drosophila genome project. Insect Biochem Mol Biol 31: 513–520. doi: 10.1016/s0965-1748(01)00035-2
  20. 20. Karger A, Kampen H, Bettin B, Dautel H, Ziller M, et al. (2012) Species determination and characterization of developmental stages of ticks by whole-animal matrix-assisted laser desorption/ionization mass spectrometry. Ticks Tick Borne Dis 3: 78–89. doi: 10.1016/j.ttbdis.2011.11.002
  21. 21. Kaufmann C, Ziegler D, Schaffner F, Carpenter S, Pfluger V, et al. (2011) Evaluation of matrix-assisted laser desorption/ionization time of flight mass spectrometry for characterization of Culicoides nubeculosus biting midges. Med Vet Entomol 25: 32–38. doi: 10.1111/j.1365-2915.2010.00927.x
  22. 22. Kaufmann C, Schaffner F, Ziegler D, Pfluger V, Mathis A (2012) Identification of field-caught Culicoides biting midges using matrix-assisted laser desorption/ionization time of flight mass spectrometry. Parasitology 139: 248–258. doi: 10.1017/s0031182011001764
  23. 23. Steinmann IC, Pfluger V, Schaffner F, Mathis A, Kaufmann C (2013) Evaluation of matrix-assisted laser desorption/ionization time of flight mass spectrometry for the identification of ceratopogonid and culicid larvae. Parasitology 140: 318–327. doi: 10.1017/s0031182012001618
  24. 24. Mazzeo MF, Giulio BD, Guerriero G, Ciarcia G, Malorni A, et al. (2008) Fish authentication by MALDI-TOF mass spectrometry. J Agric Food Chem 56: 11071–11076. doi: 10.1021/jf8021783
  25. 25. Karger A, Bettin B, Lenk M, Mettenleiter TC (2010) Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing. J Virol Methods 164 (1–2) 116–21. doi: 10.1016/j.jviromet.2009.11.022
  26. 26. Ouedraogo R, Daumas A, Ghigo E, Capo C, Mege JL, et al. (2012) Whole-cell MALDI-TOF MS: a new tool to assess the multifaceted activation of macrophages. J Proteomics 75: 5523–5532. doi: 10.1016/j.jprot.2012.07.046
  27. 27. Ouedraogo R, Flaudrops C, Ben Amara A, Capo C, Raoult D, et al. (2010) Global analysis of circulating immune cells by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. PLoS One 5: e13691. doi: 10.1371/journal.pone.0013691
  28. 28. Tran TN, Aboudharam G, Gardeisen A, Davoust B, Bocquet-Appel JP, et al. (2011) Classification of ancient mammal individuals using dental pulp MALDI-TOF MS peptide profiling. PLoS One 6: e17319. doi: 10.1371/journal.pone.0017319
  29. 29. Dubois D, Grare M, Prere MF, Segonds C, Marty N, et al. (2012) Performances of the Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for rapid identification of bacteria in routine clinical microbiology. J Clin Microbiol 50: 2568–2576. doi: 10.1128/jcm.00343-12
  30. 30. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685. doi: 10.1038/227680a0
  31. 31. Candiano G, Bruschi M, Musante L, Santucci L, Ghiggeri GM, et al. (2004) Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis 25: 1327–1333. doi: 10.1002/elps.200305844
  32. 32. Arnold RJ, Reilly JP (1998) Fingerprint matching of E. coli strains with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of whole cells using a modified correlation approach. Rapid Commun Mass Spectrom 12: 630–636. doi: 10.1002/(sici)1097-0231(19980529)12:10<630::aid-rcm206>3.3.co;2-s
  33. 33. Nayduch D, Aksoy S (2007) Refractoriness in tsetse flies (Diptera: Glossinidae) may be a matter of timing. J Med Entomol 44: 660–665. doi: 10.1603/0022-2585(2007)44[660:ritfdg]2.0.co;2
  34. 34. Motloang M, Masumu J, Mans B, Van den Bossche P (2012) Latif A (2012) Vector competence of Glossina austeni and Glossina brevipalpis for Trypanosoma congolense in KwaZulu-Natal, South Africa. Onderstepoort J Vet Res 79 (1) E1–6 doi: 10.4102/ojvr.v79i1.353.
  35. 35. Gooding RH, Krafsur ES (2005) Tsetse genetics: contributions to biology, systematics, and control of tsetse flies. Annu Rev Entomol 50: 101–123. doi: 10.1146/annurev.ento.50.071803.130443
  36. 36. Petersen FT, Meier R, Kutty SN, Wiegmann BM (2007) The phylogeny and evolution of host choice in the Hippoboscoidea (Diptera) as reconstructed using four molecular markers. Mol Phylogenet Evol 45: 111–122. doi: 10.1016/j.ympev.2007.04.023
  37. 37. Jordan AM (1974) Performance of the Langford animal-fed colonies of Glossina, 1973. Les moyens de lutte contre les trypanosomes et leur vecteur Revue d'Elevage et de Médecine Vétérinaire, Supplément 55–56.
  38. 38. Nash TA, Jordan AM, Boyle JA (1968) The large-scale rearing of Glossina austeni Newst, in the laboratory. IV. The final technique. Ann Trop Med Parasitol 62: 336–341.
  39. 39. Abd-Alla AMM, Adun H, Parker AG, Vreysen MJB, Bergoin M (2012) The Antiviral Drug Valacyclovir Successfully Suppresses Salivary Gland Hypertrophy Virus (SGHV) in Laboratory Colonies of Glossina pallidipes. PLoS ONE 7: e38417. doi: 10.1371/journal.pone.0038417
  40. 40. Itard J (1974) Situation actuelle des élevages de glossines à l'I.E.M.V.T. (Maison-Alfort). Les moyens de lutte contre les Trypanosomes et leurs vecteurs Actes du Colloque Paris 51–53.
  41. 41. Moloo SK, Kutuza SB (1988) Large-scale rearing of Glossina brevipalpis in the laboratory. Med Vet Entomol 2: 201–202. doi: 10.1111/j.1365-2915.1988.tb00074.x