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Research Article

Occurrence of Nontuberculous Mycobacterial Pulmonary Infection in an Endemic Area of Tuberculosis

  • Ana Roberta Fusco da Costa mail,

    robertafusco@gmail.com

    Affiliation: Bacteriology and Mycology Section, Evandro Chagas Institute, Ananindeua, Para, Brazil

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  • Joseph O. Falkinham III,

    Affiliation: Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, United States of America

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  • Maria Luiza Lopes,

    Affiliation: Bacteriology and Mycology Section, Evandro Chagas Institute, Ananindeua, Para, Brazil

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  • Adriana Rodrigues Barretto,

    Affiliation: João de Barros Barreto University Hospital, Federal University of Para, Belem, Para, Brazil

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  • João Soares Felicio,

    Affiliation: Department of Integrative Medicine, Federal University of Para, Belem, Para, Brazil

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  • Lúcia Helena Messias Sales,

    Affiliation: Department of Integrative Medicine, Federal University of Para, Belem, Para, Brazil

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  • Jeann Ricardo da Costa Bahia,

    Affiliation: Bacteriology and Mycology Section, Evandro Chagas Institute, Ananindeua, Para, Brazil

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  • Emilyn Costa Conceição,

    Affiliation: Bacteriology and Mycology Section, Evandro Chagas Institute, Ananindeua, Para, Brazil

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  • Karla Valéria Batista Lima

    Affiliation: Bacteriology and Mycology Section, Evandro Chagas Institute, Ananindeua, Para, Brazil

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  • Published: July 18, 2013
  • DOI: 10.1371/journal.pntd.0002340

Abstract

The majority of investigations of the epidemiology of nontuberculous mycobacteria (NTM) have focused on highly developed nations with a low prevalence of tuberculosis. In contrast, the Para state of north Brazil represents an area of high tuberculosis prevalence and increasing NTM incidence. Toward the goal of understanding the dynamics of infection by all Mycobacterium species, we report patient characteristics and the identification of NTM strains isolated from sputum samples from patients that were residents of Para, a state in the Amazon region, Northern of Brazil, over the period January 2010 through December 2011 (2 years). The 29 NTM patients comprised 13.5% of positive mycobacterial cultures over the 2-year period. A major risk factor for NTM pulmonary disease was previous tuberculosis (76%). Further, the average age of NTM patients (52 years) was significantly higher than that of tuberculosis patients (39 years) and more were female (72.4% vs. 37.4%). Unlike other Brazilian states, NTM pulmonary patients in Para were infected with a different spectrum of mycobacteria; primarily the rapidly growing Mycobacterium massiliense and Mycobacterium simiae complex.

Author Summary

Nontuberculous mycobacteria (NTM) are environmental organisms that are naturally found in soil, water, dust and other sites. Several case reports and studies on the prevalence of pulmonary NTM disease have been published, nevertheless, the impact and the exact magnitude of NTM infections in countries where tuberculosis (TB) is endemic are not known. Here, we report the identification of NTM strains isolated from pulmonary samples from patients with a presumptive diagnosis of pulmonary TB and residents of the State of Para, in the Amazon region, Northern of Brazil. This study documents the occurrence and diversity of species of NTM that cause pulmonary disease in a region representative of those in the world with high infection rates by Mycobacterium tuberculosis.

Introduction

Nontuberculous mycobacteria (NTM) are environmental opportunistic pathogens that are natural inhabitants of soil [1] and drinking water [2], [3]. Humans and their agronomic animals are literally surrounded by nontuberculous mycobacteria [4]. Risk factors for NTM pulmonary disease include: prior tuberculosis, chronic obstructive pulmonary disease (COPD), lung damage due to occupational exposures to dusts (e.g., mining), cystic fibrosis or heterozygosity for a cystic fibrosis mutation, α-1-antitrypsin deficiency [5]. Fisherman and others exposed to fish are at risk for skin infections caused by Mycobacterium marinum infection [5] and children from 18 months to 5 years of age are at risk for cervical lymphadenitis caused more typically by M. avium [6]. Immunodeficiency, due to HIV-infection or immunosuppression due to cancer or chemotherapy are risk factors for Mycobacterium avium bacteremia [2].

Several case reports and studies on the prevalence of pulmonary disease caused by NTM in North America, Europe and Japan have been published during recent years [7], [8], [9], [10], [11]. Nevertheless, the impact and the exact magnitude of NTM infections in countries where tuberculosis is endemic are not known. Here, we report the identification of NTM strains isolated from pulmonary samples from patients with a presumptive diagnosis of pulmonary TB and residents of the State of Para, in the Amazon region, Northern of Brazil. This study documents the occurrence and diversity of species of NTM that cause pulmonary disease in a region representative of those in the world with high infection rates by M. tuberculosis.

Materials and Methods

Patients and Clinical Samples

Patients from routine laboratory presenting symptoms suggestive of mycobacterial disease (e.g., chronic cough) and/or who were noted to have radiological alterations at medical examination, and from NTM were isolated at least once between January 2010 and December 2011 at the Evandro Chagas Institute, were included in this study. All the NTM isolates described in this study were obtained from pulmonary samples (sputum, bronchoalveolar washes, and gastric washes samples) of 38 individuals residents of the State of Para, North Brazil. Patient records were reviewed to assess the clinical data. Diagnostic criteria for NTM disease published by the American Thoracic Society (ATS) were applied to determine the clinical relevance of NTM isolation (Table 1) [12].

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Table 1. American Thoracic Society diagnostic criteria on nontuberculous mycobacterial pulmonary disease.

doi:10.1371/journal.pntd.0002340.t001

The clinical samples were initially decontaminated, using the N-acetyl-L-cysteine-sodium hydroxide procedure [13]. The samples were subsequently inoculated onto Löwenstein–Jensen medium (Difco, Sparks, USA) and incubated at 35°–37°C in the absence of light for at least six weeks or until colonies appeared. Isolates of the M. tuberculosis complex were distinguished from NTM by the unique breadcrumb or cauliflower colony morphology of M. tuberculosis, and the production of cord factor and susceptibility to 0.5 mg/mL of para-nitrobenzoic acid by M. tuberculosis [14].

Ethics Statement

All subjects provided written consent by signing the free and informed consent form, and all patients data analyzed were anonymized. This study was approved by the ethics committee of the Evandro Chagas Institute (protocol n° 017/10, CAAE: 0017.0.072.000-10).

Molecular Identification

All NTM isolates of this study were identified by sequencing a portion of the 16S rRNA [15] and hsp65 [16] genes.

Statistical Analysis

The descriptive analysis were expressed as mean ± standard deviation or percentage, while analytical statistics was conducted using either non-parametric Chi-squared test or G-test, using the software BioEstat version 5.01 [17]. Statistical significance was defined as p<0.05.

Results

Patient Characteristics

From January 2010 to December 2011, a total of 69 NTM isolates were recovered from pulmonary specimens from 38 patients with respiratory symptoms that included chronic cough and alterations on chest X-ray. The patients and their characteristics are listed in Table 1. The 38 patients represented 13.5% of culture-positive mycobacterial cultures obtained in our laboratory over that period of time. Of the 38 patients, 29 met the American Thoracic Society diagnostic criteria for NTM infection [12]. All patients were initially diagnosed as having pulmonary tuberculosis (M. tuberculosis) based on sputum smear microscopy for acid-fast bacilli (AFB) and had suffered a treatment failure.

A summary of the characteristics of the 29 NTM patients meeting the criteria for NTM disease is provided in Table 2. Among the patients with NTM disease the mean age was 52.3 years (±17.8 SD), and the mean time from onset of symptoms to NTM diagnosis was of 7.8 months (±13.5 SD). Twenty of the 29 patients (68.9%) were above 50 years old, whereas 69.4% of tuberculosis cases (X2 = 26.7; p<0.0001) reported in the state of Para are under 50 years old (Figure 1).

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Figure 1. Proportion of tuberculosis and nontuberculous mycobacterial lung disease by age group - State of Para, Brazil, 2010–2011.

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Table 2. Individuals who met the ATS microbiological criteria for nontuberculous mycobacterial pulmonary disease from Para State, Brazil.

doi:10.1371/journal.pntd.0002340.t002

Among the most frequent co-morbidities found were prior tuberculosis (22/29, 75.8%) and bronchiectasis (13/29, 44.8%) (Table 2). The results of chest X-ray (CXR) and high resolution computerized tomography (CT) examination are shown in Table 3 and Figure 2. A number of 17 CXR and 12 CT findings of these 29 NTM-patients were reviewed. Atelectasis (12/17, 70.5%) and cavities (7/17, 41.2%) were the most frequent findings in CXR, while bronchiectasis (12/12, 100%), centrilobular nodules/tree-in-bud (8/12, 66.6%) and cavities (6/12, 50%) were more frequently observed in the CT. Pleural thickening was detected in 8 (47.0%) patients. Clinical manifestations of advanced lung disease, such as dyspnea and haemoptysis, occurred in 15 (88.2%) patients.

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Figure 2. Representative image showing lung damage in a patient with nontuberculous mycobacterial diseases.

A 69-years-old woman with Mycobacterium abscessus pulmonary disease. (A and B) High resolution computed tomography (HRCT) of the chest obtained at level of upper lobes showing multiples cavities in the right upper lobe and centrilobular nodules. It is also possible to observe bronchiectasis in left upper lobe (arrow). (C) Tree-in-bud pattern. (D) Presence of bronchiectasis in middle lobe. Also note centrilobular nodules at right lower lobe.

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Table 3. Clinical and images findings for some nontuberculous mycobacterial pulmonary infected patients from Para State, Brazil.

doi:10.1371/journal.pntd.0002340.t003

A total 26 out of the 29 NTM-infected patients (89.6%) were classified as pardo, a Brazilian term for people of mixed white and indigenous heritage, who constitute the majority of the Para state population, with a total of 5,270,307 (69.5%) of the population in the 2010 Brazil Census [18]. The frequency of pardo individuals with NTM was significantly different from the percentage of pardo in the state of Para (X2 = 5.5; p = 0.0312) (Table 1). Among the 29 NTM-infected individuals, 21 were females (72.4%), aged between 19–84 years (50.9±18.3 SD). There was statistically significant difference in the occurrence of M. tuberculosis and NTM infection between males and females (62.6% in male with TB versus 72.4% in female with NTM; X2 = 15.1; p = 0.0002). A total of five patients declared themselves as smokers. Roughly 72% (21/29) of patients were residents from an urban area, with 64.2% (18/21) of them having access to a water supply through piped systems. The difference between NTM urban and rural residents with access to piped water supply systems was found significant (G-test = 21.3; p = 0.0001).

NTM Isolates

Eight different NTM species were identified from the 29 patients meeting the ATS criteria and included M. massiliense (n = 13; 44.8%), M. avium (n = 3; 10.3%), M. intracellulare (n = 3; 10.3%), M. abscessus (n = 2; 6.9%), M. bolletii (n = 1; 3.4%), M. moriokaense (n = 1; 3.4%), M. fortuitum (n = 1; 3.4%), M. celatum (n = 1; 3.4%) and M. kansasii (n = 1; 3.4%). Eight isolates (28%) from three patients were identified as being related to the M. simiae complex by 16S rRNA sequence. The sequences obtained shared 100% similarity with the corresponding 16S rRNA (GenBank accession number HM056101) and hsp65 gene sequences (GenBank accession number HM056135) of Mycobacterium sp. IEC23. The pulmonary infection by M. chelonae-M. abscessus complex members (M. abscessus, M. massiliense and M. bollletii) occurred in females with an average age of 60.7 years. Among the nine patients who did not meet the diagnostic criteria the NTM disease, the NTM species isolated included M. fortuitum (n = 3; 33.3%), M. avium (n = 2; 22.2%), M. gordonae (n = 1; 11.1%), M. colombiense (n = 1; 11.1%), M. intracellulare (n = 1; 11.1%) and M. abscessus (n = 1; 11.1%).

Discussion

About 80% (29/38) of all the NTM patients met the ATS criteria for NTM pulmonary disease [12]. Among nine cases that did not meet ATS criteria, one was highly suggestive of NTM infection. This patient showed both clinical symptoms of mycobacterial disease and a positive sputum smear. Such cases need to remain under observation and expert consultation sought [12].

This study clearly provides guidance in the diagnosis of NTM pulmonary disease in an area of high tuberculosis prevalence. Specifically, NTM-infected patients were older, more frequently female and had prior tuberculosis. In the Para state of Brazil, being of the pardo race was a risk factor for NTM disease. Roughly 70% of NTM pulmonary infections cases were patients over 50 years old, as other contemporary studies have shown [9], [19]. These data also agree with the characteristics of a series of NTM-infected patients that had revealed an increased NTM-disease susceptibility among female, slender and older individuals [20].

A variety of factors may contribute to the observation that prior tuberculosis was found to be a risk factor for NTM disease: (i) lung damage resulting from prior tuberculosis infection reduces normal clearing of pathogens; (ii) a proportion of tuberculosis patients are at increased risk for mycobacterial infection, and this subset of tuberculosis patients would be at risk for nontuberculous mycobacterial infection; and (iii) as M. tuberculosis infection is associated with nutritional deficiency, that subset of individuals with prior tuberculosis would be expected to be of increased susceptibility to NTM infection [21], [22], [23], [24]. COPD and cancer, diseases commonly associated with NTM disease, were less frequent in this series of case (one case of each). In the USA, COPD was described as the main co-morbidity, being found in up to 28% of the cases, while neoplastic diseases have been reported in 25% of cases [25], [26].

The fact that the average age of M. tuberculosis-infected patients was lower than that of the NTM patients is likely due to a number of reasons. First, M. tuberculosis is a highly virulent pathogen, capable of infecting healthy individuals; thus persons of all ages are susceptible. In contrast, the NTM are opportunistic pathogens; every NTM patient has some risk factor for infection. In developed countries, NTM disease is more frequently seen in older (>60 years), slender (<50 kg) men and women who lack risk factors for M. tuberculosis infection [20]. All risk factors for NTM disease are unknown, although it has been shown that they are innately susceptible, as they are subject to repeated NTM infection [22], [24], [27], [28], [29]. In recent publication, Dirac et al [30] reported that prior lung disease and immunosuppression appear to be associated with susceptibility to NTM disease. Furthermore, it is well-known that elderly individuals generally have a worse response to infections than the young ones, possibly as the result of the immunosenescence. This condition has been associated to an increased susceptibility to infections, including mycobacterial infections [31], [32].

A low proportion of HIV infected patients was observed among the NTM patients, as proven by serology in this study. However, this finding does not rule out the possibility of NTM-HIV co-infection in the study area, but instead it may point to the possibility of death of these patients by other causes, or perhaps even by disseminated NTM-infections, before appearance of respiratory NTM disease.

Similar results have been found in Rio de Janeiro State, where 9.8% of NTM cases were diagnosed in HIV infected patients [33]. Even smaller proportion was found in the USA and Denmark, with 3.4% and 2.4% of HIV infected patients, respectively [26], [34]. According to Sexton et al. [35], this low frequency suggests that an abnormal airway mucosa is required as initiating factor for NTM disease. Among the HIV-infected patients in this study, all of them had history of previous tuberculosis and additionally smoking, co-morbidities that predispose to NTM pulmonary disease.

NTM patients had a lower frequency of cavitary lesions compared to tuberculosis patients (Table 4). Although the radiographic features of NTM pulmonary infections are similar to those of tuberculosis, the presence of upper lobe cavitary lesions and endobronchial spread, bronchiectasis, as well as of fibroproductive nodules that change slowly, cicatricial atelectasis, and pleural thickening, were common findings in the patients of this study, which also had been shown in other studies [12], [36], [37]. The radiologic manifestations of pulmonary NTM has been classified basically as both cavitary (“classic”) or nodular-bronchiectatic (“nonclassic”) forms [37], [38], [39]. However, some NTM-cases could not be securely to fit into these categories, since they have exhibited the two forms combined. Others studies have tried to associate the patterns and forms of pulmonary lesions to NTM-species, suggesting a radiological differentiation between the diseases caused by MAC and M. abscessus. Briefly, has been proposed that nodular-bronquiectatic form is more frequent in patients with M. abscessus infection, while in those with MAC-infection the airspace consolidation and cavities are the most common findings [40], [41], [42], [43], [44], [45], [46], [47]. However, these presentations did not agree with our results in all cases.

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Table 4. Summary of distinguishing features of NTM patients.

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We found that the majority of the patients were of the pardo race. The percentage of the NTM-patients reported here (89.6%) is considerably higher than the percentage of pardo individuals in the state of Pará (69.5%). This could be due to either increased susceptibility of pardo individuals to NTM disease or greater opportunity of exposure to NTM-sources such as agricultural soils or drinking water [3], [48]. Assignment of increased susceptibility is problematic as pardo individuals represent a heterogeneous, genetically diverse group. In this study we found 64.2% of the patients having access to a water supply through piped systems. This information is important because, even in urban area, as in Belém – capital of the State of Pará, the water supply is still precarious, with approximately 75% of homes having access to a water supply through piped systems, being the lowest coverage of them in the peripheral urban areas, according to the 2010 Brazil Census [18]. Most patients reported in this study were residents of peripheral urban areas (data not shown).

Based in the 16S rRNA sequencing analysis, a group of five isolates was classified as M. simiae complex (MSC). Among the MSC members, only M. simiae species is recognized as potentially pathogenic to human and it is most commonly associated to cervical lymphadenitis. Nevertheless, the recovery of M. simiae from pulmonary specimens has been reported, especially in Israel, Cuba, and the southwestern United States [12]. Similarly, in previous study we found in our laboratory strains phylogenetically related to MSC as the most frequent NTM in pulmonary infection in Para State, Brazil [49], [50].

RGM species, including M. abscessus, M. massiliense, M. bolletii (formally M. abscessus species) represented almost 45% of all NTM pulmonary cases, whereas in Sao Paulo, M. avium complex (MAC) and M. kansasii represent the most common NTM in pulmonary disease [51], [52]. In addition, when stratifying to the NTM species level, we observed that gender associated infection was even more pronounced in the case of M. chelonae-M. abscessus complex (~45% females), especially M. massiliense (34.5%). Griffith et al. [53] found a predominance of females (65%) among 154 cases of pulmonary disease by RGM, while descriptions of particular forms of pulmonary disease caused by MAC in women have been reported [54], [55]. Further studies are needed to elucidate the reasons for female susceptibility.

A number of factors may have contributed to the higher frequency of individuals infected with M. massiliense than reported in other studies. First, M. massiliense may be more common in the soils and waters of the Para State of Brazil. Second, M. massiliense is a newly described species; unknown to investigators until recently [56]. Therefore, as M. massiliense and M. abscessus share a number of common characteristics that are used for identification, earlier published studies may have misidentified M. massiliense isolates as M. abscessus. Mycobacterial taxonomy has been undergoing substantial revision; for example it has recently been shown that standard tests for identification cannot distinguish between M. intracellulare and the newly described M. chimaera [57]. That, in turn, has led to the discovery that all water isolates of M. intracellulare are really M. chimaera, forcing a re-evaluation of M. intracellulare epidemiology and ecology [58]. In the present instance, the recent discovery of M. massiliense and its separation from M. abscessus suggests that earlier reports around the world reporting the frequency and numbers of M. abscessus infections may be incorrect; the isolates could have been M. massiliense. Thus, prior reports that form the basis for judging that the frequency of M. massiliense infections in the Para State is high may be incorrect.

The results this study show that the clinical manifestations presented by the NTM-patients are suggestive of advanced disease, which reinforces the importance of the timely diagnosis of the NTM disease, since delayed treatment is associated with severe morbidity.

Acknowledgments

The authors would like to thank Maurimélia Mesquita da Costa for excellent technical assistance in the preparation of this paper.

Author Contributions

Conceived and designed the experiments: ARFdC JOF. Performed the experiments: ARFdC JRdCB MLL ECC. Analyzed the data: ARFdC JOF ARB JSF LHMS KVBL. Contributed reagents/materials/analysis tools: ARFdC MLL KVBL. Wrote the paper: ARFdC JOF ARB.

References

  1. 1. Brooks RW, George KL, Parker BC, Falkinham JO 3rd, Gruff H (1984) Recovery and survival nontuberculous mycobacteria under various growth and decontamination conditions. Can J Microbiol 30(9): 1112–7. doi: 10.1139/m84-174
  2. 2. Falkinham JO 3rd, Parker BC, Gruft H (1980) Epidemiology of infection by nontuberculous mycobacteria. I. Geographic distribution in the eastern United States. Am Rev Respir Dis 121(6): 931–7.
  3. 3. Falkinham JO 3rd, Norton CD, LeChevallier MW (2001) Factors influencing numbers of Mycobacterium avium, Mycobacterium intracellulare, and other mycobacteria in drinking water distribution systems. Appl. Environ. Microbiol 67: 1225–1231. doi: 10.1128/aem.67.3.1225-1231.2001
  4. 4. Falkinham JO 3rd (2009) Surrounded by mycobacteria: nontuberculous mycobacteria in the human environment. J Appl Microbiol 107(2): 356–67. doi: 10.1111/j.1365-2672.2009.04161.x
  5. 5. Marras TK, Daley CL (2002) Epidemiology of human pulmonary infection with nontuberculous mycobacteria. Clin Chest Med 23(3): 553–67. doi: 10.1016/s0272-5231(02)00019-9
  6. 6. Wolinsky E (1995) Mycobacterial lymphadenitis in children: a prospective study of 105 nontuberculous cases with long-term follow-up. Clin Infect Dis 20(4): 954–63. doi: 10.1093/clinids/20.4.954
  7. 7. Tsukamura M, Shimoide H, Kita N, Kawakami K, Ito T (1981) Epidemiologic studies of lung disease due to mycobacteria other than Mycobacterium tuberculosis in Japan. Rev Infect Dis 3(5): 997–1007. doi: 10.1093/clinids/3.5.997
  8. 8. Iseman MD, Marras TK (2008) The importance of nontuberculous mycobacterial lung disease. Am J Respir Crit Care Med 178(10): 999–1000. doi: 10.1164/rccm.200808-1258ed
  9. 9. Billinger ME, Olivier KN, Viboud C, de Oca RM, Steiner C, et al. (2009) Nontuberculous mycobacteria-associated lung disease in hospitalized persons, United States, 1998–2005. Emerg Infect Dis 15(10): 1562–9. doi: 10.3201/eid1510.090196
  10. 10. Thomson RM (2010) Changing epidemiology of pulmonary nontuberculous mycobacteria infections. Emerg Infect Dis 16(10) : 1576–83. doi: 10.3201/eid1610.091201
  11. 11. Kendall BA, Varley CD, Choi D, Cassidy PM, Hedberg K, et al. (2011) Distinguishing tuberculosis from nontuberculous mycobacteria lung disease, Oregon, USA. Emerg Infect Dis 17(3): 506–9. doi: 10.3201/eid1703.101164
  12. 12. Griffith DE, Aksamit T, Brown-Elliott BA, Catanzaro A, Daley C, et al. (2007) An official ATS/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. Am J Respir Crit Care Med 175(4): 367–416. doi: 10.1164/rccm.200604-571st
  13. 13. Webb WR (1962) Clinical evaluaton of a new mucolytic agent, acetyl-cysteine. J Thorac Cardiovasc Surg 44: 330–43.
  14. 14. Kubica GP (1973) Differential identification of mycobacteria. Am Rev Resp Dis 107: 9–12.
  15. 15. Shin S, Kim EC, Yoon JH (2006) Identification of nontuberculous mycobacteria by sequence analysis of the 16S ribosomal RNA, the heatshock protein 65 and the RNA polymerase β-subunit genes. Korean J Lab Med 26: 153–160. doi: 10.3343/kjlm.2006.26.3.153
  16. 16. Kim H, Kim SH, Shim TS, Kim M, Bai GH, et al. (2005) Differentiation of Mycobacterium species by analysis of the heat-shock protein 65 gene (hsp65). Int J Syst Evol Microbiol 55: 1649–1656. doi: 10.1099/ijs.0.63553-0
  17. 17. Ayres M, Ayres Jr M, Ayres AL, Santos AS (2007) BioEstat 5.0. Aplicações estatísticas nas áreas das ciências biológicas e médicas, Instituto de Desenvolvimento Sustentável Mamirauá - IDSM/MCT/CNPq, Belém. 324 pp. [URL: http://www.mamiraua.org.br/downloads/pro​gramas/]
  18. 18. IBGE – Instituto Brasileiro de Geografia e Estatítica. Censo 2010. [URL: http://censo2010.ibge.gov.br/resultados].
  19. 19. Cook JL (2010) Nontuberculous mycobacteria: opportunistic environmental pathogens for predisposed hosts. Br Med Bull 96: 45–59. doi: 10.1093/bmb/ldq035
  20. 20. Prince DS, Peterson DD, Steiner RM, Gottlieb JE, Scott R, et al. (1989) Infection with Mycobacterium avium complex in patients without predisposing conditions. N Engl J Med 321(13): 863–8. doi: 10.1056/nejm198909283211304
  21. 21. Wolinsky E (1979) Nontuberculous mycobacteria and associated diseases. Am Rev Dis 119(1): 107–59.
  22. 22. Marras TK, Daley CL (2002) Epidemiology of human pulmonary infection with nontuberculous mycobacteria. Clin Chest Med 23(3): 553–67. doi: 10.1016/s0272-5231(02)00019-9
  23. 23. Gupta KB, Gupta R, Atreja A, Verma M, Vishvkarma S (2009) Tuberculosis and nutrition. Lung India 26(1): 9–16. doi: 10.4103/0970-2113.45198
  24. 24. Gruft H, Falkinham JO 3rd, Parker BC (1981) Recent experience in the epidemiology of disease caused by atypical mycobacteria. Rev Infect Dis 3(5): 990–6. doi: 10.1093/clinids/3.5.990
  25. 25. Winthrop KL, McNelley E, Kendall B, Marshall-Olson A, Morris C, et al. (2010) Pulmonary nontuberculous mycobacterial disease prevalence and clinical features: an emerging public health disease. Am J Respir Crit Care Med 182(7): 977–82. doi: 10.1164/rccm.201003-0503oc
  26. 26. Prevots DR, Shaw PA, Strickland D, Jackson LA, Raebel MA (2010) Nontuberculous mycobacterial lung disease prevalence at four integrated health care delivery systems. Am J Respir Crit Care Med 182(7): 970–6. doi: 10.1164/rccm.201002-0310oc
  27. 27. O'Brien RJ, Geiter LJ, Snider DE Jr (1987) The epidemiology of nontuberculous mycobacterial diseases in the United States. Results from a national survey. Am Rev Respir Dis 135(5): 1007–14.
  28. 28. Cassidy PM, Hedberg K, Saulson A, McNelly E, Winthrop KL (2009) Nontuberculous mycobacterial disease prevalence and risk factors: a changing epidemiology. Clin Infect Dis 49(12): e124–9. doi: 10.1086/648443
  29. 29. Winthrop KL, McNelley E, Kendall B, Marshall-Olson A, Morris C, et al. (2010) Pulmonary nontuberculous mycobacterial disease prevalence and clinical features: an emerging public health disease. Am J Respir Crit Care Med 182(7): 977–82. doi: 10.1164/rccm.201003-0503oc
  30. 30. Dirac MA, Horan KL, Doody DR, Meschke JS, Park DR, et al. (2012) Environment or host?: A case-control study of risk factors for Mycobacterium avium complex lung disease. Am J Respir Crit Care Med 186(7): 684–91. doi: 10.1164/rccm.201205-0825oc
  31. 31. Busse PJ, Mathur SK (2010) Age-related Changes in Immune Function: Impact on Airway Inflammation. J Allergy Clin Immunol 126(4): 690–701. doi: 10.1016/j.jaci.2010.08.011
  32. 32. Ongrádi J, Kövesdi V (2010) Factors that may impact on immunosenescence: an appraisal. Immun Ageing 7: 7. doi: 10.1186/1742-4933-7-7
  33. 33. Mello K, Mello F, Borga L, Duarte R, Sampaio E, et al. (2013) Clinical and Therapeutic Features of Pulmonary Nontuberculous Mycobacterial Disease, Rio de Janeiro, Brazil. Emerg Infect Dis 19(3): 393–399. doi: 10.3201/eid1903.120735
  34. 34. Andréjak C, Thomsen VØ, Johansen IS, Riis A, Benfield TL, et al. (2010) Nontuberculous pulmonary mycobacteriosis in Denmark: incidence and prognostic factors. Am J Respir Crit Care Med 181(5): 514–21. doi: 10.1164/rccm.200905-0778oc
  35. 35. Sexton P, Harrison AC (2008) Susceptibility to nontuberculous mycobacterial lung disease. Eur Respir J 31(6): 1322–33. doi: 10.1183/09031936.00140007
  36. 36. Woodring JH, Vandiviere HM (1990) Pulmonary disease caused by nontuberculous mycobacteria. J Thorac Imaging 5(2): 64–76. doi: 10.1097/00005382-199004000-00009
  37. 37. Martinez S, McAdams HP, Batchu CS (2007) The many faces of pulmonary nontuberculous mycobacterial infection. AJR Am J Roentgenol 189(1): 177–86. doi: 10.2214/ajr.07.2074
  38. 38. Miller WT Jr (1994) Spectrum of pulmonary nontuberculous mycobacterial infection. Radiology 191: 343–50.
  39. 39. Ellis SM, Hansell DM (2002) Imaging of non-tuberculous (atypical) mycobacterial pulmonary infection. Clin Radiol 57: 661–69. doi: 10.1053/crad.2001.0851
  40. 40. Albelda SM, Kern JA, Marinelli DL, Miller WT (1985) Expanding spectrum of pulmonary disease caused by nontuberculous mycobacteria. Radiology 157: 289–96.
  41. 41. Chung MJ, Loo KS, Koh WJ (2005) Thin-section CT-findings of nontuberculous mycobacterial diseases: comparisson between Mycobacterium avium-intracellulare complex and Mycobacterium abscessus infection. J Korean Med Sci 20: 777–83. doi: 10.3346/jkms.2005.20.5.777
  42. 42. Lynch DA, Simone PM, Fox MA, Bucher BL, Heinig MJ (1995) CT features of pulmonary Mycobacterium avium complex infection. J Comput Assist Tomogr 19: 353–60. doi: 10.1097/00004728-199505000-00003
  43. 43. Obayashi Y, Fujita J, Suemitsu I, Kamei T, Nii M, et al. (1999) Sucessive follow-up of chest computed tomography in patients with Mycobacterium avium-intracellulare complex. Respir Med 93: 11–5. doi: 10.1016/s0954-6111(99)90070-7
  44. 44. Fujiuchi S, Matsumoto H, Yamazaki Y, Nakao S, Takanashi M, et al. (2003) Analysis of chest CT in patients with Mycobacterium avium complex pulmonary disease. Respiration 70: 76–81. doi: 10.1159/000068416
  45. 45. Wittram C, Weisbrod GL (2002) Mycobacterium avium complex lung disease in immunocompetent patients: radiography-CT correlation. Br J Radiol 75(892): 340–4.
  46. 46. Koh WJ, Lee KS, Kwon OJ, Jeong YJ, Kwak SH, et al. (2005) Bilateral bronchiectasis and bronchiolitis at thin-section CT: diagnostic implications in nontuberculous mycobacterial pulmonary infection. Radiology 235(1): 282–8. doi: 10.1148/radiol.2351040371
  47. 47. Hans D, Lee KS, Koh WJ, Yi CA, Kim TS, et al. (2003) Radiographic and CT findings of nontuberculous mycobacteria pulmonary infection caused by Mycobacterium abscessus. AJR Am J Roentgenol 181(2): 513–7. doi: 10.2214/ajr.181.2.1810513
  48. 48. Falkinham JO 3rd (2011) Nontuberculous mycobacteria from household plumbing of patients with nontuberculous mycobacteria disease. Emerg Infect Dis 17(3): 419–24. doi: 10.3201/eid1703.101510
  49. 49. da Costa AR, Lopes ML, Furlaneto IP, de Sousa MS, Lima KV (2010) Molecular identification of nontuberculous mycobacteria isolates in a Brazilian mycobacteria reference laboratory. Diagn Microbiol Infect Dis 68(4): 390–4. doi: 10.1016/j.diagmicrobio.2010.07.019
  50. 50. da Costa AR, Lopes ML, Leão SC, Schneider MP, de Sousa MS, et al. (2009) Molecular identification of rapidly growing mycobacteria isolates from pulmonary specimens of patients in the State of Pará, Amazon region, Brazil. Diagn Microbiol Infect Dis 65(4): 358–64. doi: 10.1016/j.diagmicrobio.2009.08.003
  51. 51. Ueki SYM, Telles MAS, Virgilio MC, Giampaglia CMS, Chimara E, et al. (2005) Nontuberculous mycobacteria: species diversity in São Paulo state, Brazil. J Bras Patol Med Lab 41(1): 1–8. doi: 10.1590/s1676-24442005000100003
  52. 52. Zamarioli LA, Coelho AG, Pereira CM, Nascimento AC, Ueki SY, et al. (2008) Descriptive study of the frequency of nontuberculous mycobacteria in the Baixada Santista region of the state of São Paulo, Brazil. J Bras Pneumol 34(8): 590–4. doi: 10.1590/s1806-37132008000800008
  53. 53. Griffith DE, Girard WM, Wallace RJ Jr (2003) Clinical features of pulmonary disease caused by rapidly growing mycobacteria. An analysis of 154 patients. Am Rev Respir Dis 147(5): 1271–8. doi: 10.1164/ajrccm/147.5.1271
  54. 54. Wallace RJ Jr (1994) Recent changes in taxonomy and disease manifestations of the rapidly growing mycobacteria. Eur J Clin Microbiol Infect Dis 13(11): 953–60. doi: 10.1007/bf02111497
  55. 55. Reich JM, Johnson RE (1991) Mycobacterium avium complex pulmonary disease. Incidence, presentation, and response to therapy in a community setting. Am Rev Respir Dis 143(6): 1381–5. doi: 10.1164/ajrccm/143.6.1381
  56. 56. Adékambi T, Reynaud-Gaubert M, Greub G, Gevaudan MJ, La Scola B, et al. (2004) Amoebal coculture of Mycobacterium massiliense sp. nov. from the sputum of a patient with hemoptoic pneumonia. J Clin Microbiol 42(12): 5493–501. doi: 10.1128/jcm.42.12.5493-5501.2004
  57. 57. Tortoli E, Rindi L, Garcia MJ, Chiaradonna P, Dei R, et al. (2004) Proposal to elevate the genetic variant MAC-A, included in the Mycobacterium avium complex, to species rank as Mycobacterium chimaera sp. nov. Int J Syst Evol Microbiol 54(Pt 4): 1277–85. doi: 10.1099/ijs.0.02777-0
  58. 58. Wallace RJ Jr, Iakhiaeva E, Williams MD, Brown-Elliott BA, Vasireddy S, et al. (2013) Absence of Mycobacterium intracellulare and presence of Mycobacterium chimaera in household water and biofilm samples of patients in the United States with Mycobacterium avium complex respiratory disease. J Clin Microbiol 51(6): 1747–52. doi: 10.1128/jcm.00186-13