The scarcity of information on the immature stages of sand flies and their preferred breeding sites has resulted in the focus of vectorial control on the adult stage using residual insecticide house-spraying. This strategy, along with the treatment of human cases and the euthanasia of infected dogs, has proven inefficient and visceral leishmaniasis continues to expand in Brazil. Identifying the breeding sites of sand flies is essential to the understanding of the vector's population dynamic and could be used to develop novel control strategies.
In the present study, an intensive search for the breeding sites of Lutzomyia longipalpis was conducted in urban and peri-urban areas of two municipalities, Promissão and Dracena, which are endemic for visceral leishmaniasis in São Paulo State, Brazil. During an exploratory period, a total of 962 soil emergence traps were used to investigate possible peridomiciliary breeding site microhabitats such as: leaf litter under tree, chicken sheds, other animal sheds and uncovered debris. A total of 160 sand flies were collected and 148 (92.5%) were L. longipalpis. In Promissão the proportion of chicken sheds positive was significantly higher than in leaf litter under trees. Chicken shed microhabitats presented the highest density of L. longipalpis in both municipalities: 17.29 and 5.71 individuals per square meter sampled in Promissão and Dracena respectively. A contagious spatial distribution pattern of L. longipalpis was identified in the emergence traps located in the chicken sheds.
The results indicate that chicken sheds are the preferential breeding site for L. longipalpis in the present study areas. Thus, control measures targeting the immature stages in chicken sheds could have a great effect on reducing the number of adult flies and consequently the transmission rate of Leishmania (Leishmania) infantum chagasi.
Sand flies are the vectors of a number of pathogens that infect man, especially cutaneous and visceral leishmaniasis. The control of sand flies through residual house-spraying insecticide has proven inefficient in preventing the spread of these diseases in several areas of the globe. Sand flies have a life cycle including eggs, larvae and pupae that are found in the ground, and winged adults. We developed a soil emergence trap that allowed us to identify chicken sheds as the preferred breeding sites for L. longipalpis that is the vector of visceral leishmaniasis in urban areas of municipalities in South-eastern region of Brazil. This finding opens up the possibility of controlling the immature stages which could complement the usual control strategies that focus on the adult fly.
Citation: Casanova C, Andrighetti MTM, Sampaio SMP, Marcoris MLG, Colla-Jacques FE, et al. (2013) Larval Breeding Sites of Lutzomyia longipalpis (Diptera: Psychodidae) in Visceral Leishmaniasis Endemic Urban Areas in Southeastern Brazil. PLoS Negl Trop Dis 7(9): e2443. doi:10.1371/journal.pntd.0002443
Editor: Paul Andrew Bates, Lancaster University, United Kingdom
Received: March 12, 2013; Accepted: August 12, 2013; Published: September 19, 2013
Copyright: © 2013 Casanova et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This reasech was supported by Superintendência de Controle de Endemias. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Members of the Lutzomyia longipalpis (Lutz & Neiva) species complex are the main vector of Leishmania (Leishmania) infantum chagasi (Cunha & Chagas), the causative agent of the visceral leishmaniasis (VL) in Brazil and Latin America –. Its recent introduction and adaptation to domiciliary habitats in the urban and peri-urban areas of municipalities in every region of Brazil, including São Paulo state, has resulted in higher incidences of human and/or canine visceral leishmaniasis –. From 1995 to 2010, Brazil recorded 53,633 new cases of the disease, with an annual mean of 3,352 new cases . From 1999 to 2011, São Paulo state recorded 1,927 new cases of the disease, with 169 deaths .
In Brazil, the VL control program focuses on the treatment of human cases, the euthanasia of seropositive infected dogs and insect vector control , , . Since this disease is zoonotic, treatment of human cases does not affect transmission. The incidence of human infections seems to be associated with the number of infected dogs and vectors –. Culling dogs is unpopular and its impact on the reduction of the incidence of human VL is contradictory –, –. Vectorial control is focused on the sand fly's adult stage through residual house-spraying insecticide , , , , . Insecticide application is generally accepted by residents in affected areas, is more practical and, in theory, more effective than the other methods at controlling transmission . However, due to the irregularity and low coverage of application, possible repellent action of insecticide, high cost when used on a large scale and its short residual effect this method is considered inefficient in the control of VL , , , .
In Brazil, the high abundance of L. longipalpis in conjunction with domestic animals, particularly dogs, which act as the amplification hosts for L. (L). i. chagasi, means that transmission occurs within relatively small areas represented by peridomiciles in rural and urban areas , –. In addition, dispersion studies have shown that the movements of L. longipalpis individuals are spatially focal , . Such observations, coupled with the high frequency of males and females of different physiological age (empty, engorged and gravid) present in collections carried out in urban areas of several municipalities of São Paulo state (unpublished), indicate that the complete life cycle of L. longipalpis occurs in peridomicile habitats of these areas. Nevertheless, finding the preferred breeding microhabitat of the L. longipalpis and other sand flies species is a difficult task –.
It is generally assumed that the immature stages of sand flies develop in shaded and moist terrestrial microhabitats, rich in organic nutrients, such as in or near leaf litter, bases of trees, animal burrows, animal sheds, and rock crevices , –. Nevertheless, there is no precise information on the preferred breeding sites of these important vectors. Among the techniques used to identify the natural breeding sites of sand flies, soil emergence traps are the preferred indirect method employed , , , , –.
In this study, a new soil emergence trap design was used to identify the natural breeding sites of L. longipalpis in urban and peri-urban areas of two municipalities identified as endemic for VL in São Paulo state. A more detailed knowledge of the immature stages and their preferential breeding sites is essential to understand the vector's population dynamics and could be used to develop novel control strategies.
The municipality of Promissão (21°32′S 49°51′W) and Dracena (21°28′S 51°31′W) are located in the Western region of São Paulo state, Brazil (Fig. 1). Promissão has a total area of 787 km2 and approximately 37,570 inhabitants. Dracena has a total area of 489 km2 and approximately 41,000 inhabitants. According to Köeppen's climate classification, these areas are identified as Aw - Tropical wet and dry . Lutzomyia longipalpis was first detected in Promissão and Dracena in 1999 and 2003, respectively. At the time of the present study, canine and human transmissions have been established in both municipalities. The houses in the urban and peri-urban areas of these municipalities usually have non-paved peridomicile with bushes and trees (e.g. fruit trees), and domestic animals shelters for dogs and chickens are common. In both municipalities, of the 20 blocks where the study was performed, chickens and dogs were present in approximately 10% and 30% of the dwellings respectively.
Figure 1. Localization of municipalities where breeding sites were investigated.doi:10.1371/journal.pntd.0002443.g001
Soil emergence traps
Two emergence trap designs were used in this study. The first one was that described by Casanova (2001) and a new one (described below) was developed by the same author (Fig. 2A–2D) through modifications in the polyvinyl chloride (PVC) pipe tube trap described by Ferro et al. (1997) , . The emergence traps were 20 cm tall, with varying diameters of 10.16, 20.32, 25.40 and 44.60 cm, sampling 0.008, 0.032, 0.051 and 0.156 m2 of substrate respectively (the diameter reduced where necessary due to site conditions). The bottom edges of the PVC tubes were serrated to allow insertion at least 5 cm into the soil (Fig. 2A) of different microhabitats. After fixing it to the ground, the inner surface of the superior part of the tube was totally covered with a 4 cm wide adhesive paper (Fly – Catcher “The Stable”, Silva – made in Sweden), which was attached to the tube wall using paper clips (Fig. 2B). To prevent sand flies escaping, the top of the tube was covered with voile (a fine-mesh, fabric gauze) fixed with rubber bands (Fig. 2C). This way, the sand flies which emerge and fly inside the PVC tube end up landing on the adhesive paper, which works as a sticky trap. Finally, to protect the trap from the sun and rain, a small tent was placed over them, taking care to leave approximately 5 cm clearance between the trap and the tent walls (Fig. 2D).
Figure 2. Emergence traps.
A: Fixing emergence trap to the ground. The serrated bottom facilitates its fixation in the soil. B: Placing the adhesive paper. C: Covering the emergence trap with a sand fly-proof fine mesh to prevent sand flies escaping. D: Emergence traps covered with a small tent.doi:10.1371/journal.pntd.0002443.g002
Two series of continuous soil emergence trap collections were carried out from March 2005 to February 2006 and from September 2006 to January 2007 in the urban and periurban area of the municipalities. The first period was exploratory and its main objective was to identify possible L. longipalpis breeding sites and the fauna associated with them. The second period aimed to evaluate the spatial distribution pattern of immature stages in the two principal microhabitats positive to L. longipalpis in Promissão, during the first study period.
First series of collections.
Five to 13 emergence traps of different sizes were set in different microhabitats of 21 and 18 peridomiciles in Promissão and Dracena respectively, for at least 84 consecutive days. In the same microhabitat, the distance between the emergence traps varied, but they were set a minimum of 40 cm away from each other. All owners had given permission for the study to be conducted on their land. The dwellings were chosen according to both the presence of adult L. longipalpis in previous collections using CDC automatic light traps, and to the environmental characteristics that indicate the presence of probable sand fly breeding sites: non-paved and shaded peridomicile with organic material from animal or vegetables in decomposition stage. The main investigated microhabitats in this exploratory period were: a) leaf litter under tree (Fig. 3A); b) chicken shed (including chickens roosted in open-fronted roost adjoining brick walls and trees) (Fig. 3B); c) other animal sheds (pig, duck, dog and rabbit); and d) uncovered debris (pile of dry branch and logs of trees and leftover construction material) (Fig. 3C). Considering a mean time of 40 days for development of immature stages of L. longipalpis under laboratory conditions (data not published) the emergence traps were maintained in the same microhabitat point for 42 days (one cycle). After this period, the emergence traps were relocated to another point (about 10 cm) of the same microhabitat for a new 42-day cycle and were considered as new traps, because they sampled another area adjacent to that one in the previous cycle in the same site. The adhesive papers were removed and examined under a stereomicroscope for the presence of sand flies every 21 days. The flies captured on the adhesive papers were carefully removed with a brush soaked in xylene and kept for two hours in a vial with xylene to remove the glue. All sand flies were identified according to Galati (2003) .
Figure 3. Examples of microhabitats.
A: Leaf-litter microhabitat. B: Chicken shed microhabitat. C: Uncovered debris microhabitat.doi:10.1371/journal.pntd.0002443.g003
To obtain preliminary information about the fauna associated with the breeding sites, soil samples (0.09 m2 and 5 cm deep) were taken with a hoe, adjacent to the emergence traps positive for L. longipalpis in Promissão. These samples were transferred to plastic bags and taken to the laboratory. The immature and adult stages of arthropods were separated in Macfayden's Funnel , maintained in ethanol (70%) and identified to Order or Family taxa, when possible. Data on the arthropods found on the adhesive papers of emergence trap positive for L. longipalpis was also assessed.
In order to monitor the adult population in the study areas of both municipalities, automatic CDC light traps were used weekly from 6 p.m. to 8 a.m. in the intradomicile and peridomicile habitats of 20 dwellings located, preferentially, on the blocks where the emergence traps were set. The distance between the CDC light traps and the emergence traps varied from five to 50 m, depending on whether the CDC light trap was in the same peridomicile or another in the same block and was influenced mainly by the relative size of the peridomiciles. The traps were installed near domestic animal shelters, preferably in chicken sheds. The monthly distribution of sand flies collected in CDC light traps and emergence trap was analyzed relative to the 10-day soil water balance . Climate related data was provided by CIIAGRO (2012) and the Agricultural Climatology Department of Campinas Agronomical Institute .
Second series of collections.
From September 2006 to January 2007, soil emergence traps were set in two chicken sheds and two leaf litter under tree microhabitats of one dwelling in Promissão. This dwelling was selected for its relatively high L. longipalpis production and high species diversity during the first series of emergence trap collection. A string grid was built over those microhabitats to divide the sampling area into 1×1 m (1 m2) quadrats (Fig. 3A). A 20.32 cm diameter (0.032 m2) emergence trap was set at random in each of the 24 quadrats of the first chicken shed and in the 15 quadrats of the second. Others 24 emergence trap, with a 25.40 cm diameter (0.051 m2), was also set in each quadrat of the two leaf litter under tree microhabitats. In contrast to the first period, in this part of the study emergence traps were maintained in the same point for 63-day periods.
Chi-square analysis, used to compare the proportion of positive microhabitats for L. longipalpis in each municipality, and Pearson correlation coefficient, calculated to examine the relationship between the number of L. longipalpis collected in emergence and CDC traps, were carried out using BioEstat (version 5.0; Mamirauá/CNPq, Belém, PA, Brazil). To evaluate the spatial distribution pattern of the immature stage, the variance-to-mean ratio (s2/), with chi-square statistic test to determining significantly departure from randomness χ2 = SS/ where s2 is the variance and is the sampling mean and SS is the sum of squares) and the Morisita's Index (Id = nΣ x2−N/N (N−1), with chi-square statistic test to determine significant departure from randomness χ2 = (nΣ x2/N)−N, where n = number of samples; N is the total number of individuals collected, and Σ x2 is the squares of the number of individuals per sample, summed over all samples) were used . In these indexes, values equal the unity indicate a random disposition, values smaller than the unity show a regular or uniform distribution, and values significantly higher than one indicate an aggregated or contagious disposition. The frequency of sand flies in CDC traps was obtained by month using William's geometric mean .
First period of study
Emergence traps in Promissão.
from March 2005 to February 2006, the leaf litter under tree microhabitat was investigated in all the 21 dwellings (61 sites), the chicken shed microhabitat was investigated in 15 (21 sites), the debris microhabitat in 13 (15 sites), and other animal sheds microhabitats in five (three rabbit hutches, one duck shed, and one pigsty). The relative low number of other animal shelters was due to the scarcity of other kinds of animals in peridomicile, excepting dogs. Although dogs were fairly common (there were dogs in about 30% of the dwelling in the study areas), their main function is usually to guard the dwelling. As such, the dogs were usually free in the peridomicile, sleeping in porches or utility areas whose floor are often concreted and cannot act as breeding sites.
A total of 386 emergence traps were used to sampled 26.92 m2 of soil: 15.62 m2 in leaf litter and base of tree trunks, 6.65 m2 in chicken sheds, 3.68 m2 in uncovered debris and 0.97 m2 in other animal sheds (pig, duck, and rabbit) (Table 1). Although the proportion of positives emergence traps for sand flies was low (5.95%), breeding sites were detected in approximately 40% of studied dwellings and all of them had one or more chicken sheds. Eleven (52.38%) chicken shed and four (6.56%) leaf litter under tree microhabitats were positive for sand flies species. The proportion of chicken sheds positive to L. longipalpis was significantly higher than in leaf litter under tree (χ2 = 20.39, df = 1, p<0.001) (Table 1).
Table 1. Summary of Lutzomyia longipalpis catches in soil emergence traps in the municipality of Promissão, from March/2005 to February/2006.doi:10.1371/journal.pntd.0002443.t001
Of the 131 sand flies collected in emergence traps, 119 (90.84%) were L. longipalpis, six (4.58%) Evandromyia cortelezzii (Brèthes 1923), three (2.29%) Evandromyia lenti (Mangabeira 1938), two (1.53%) Evandromyia termitophila (Martins, Falcão & Silva 1964), and one (0.76%) Nyssomyia neivai (Pinto 1926). Except for four L. longipalpis collected in the leaf litter under tree microhabitat, all the other sand flies were collected in the chicken sheds microhabitat.
The L. longipalpis male/female ratio was 1:1 in the leaf litter under tree microhabitat and 3.8:1 in chicken shed (Table 1). The frequency distribution analysis showed that 70 (60.87%) L. longipalpis individuals were collected in only four (with 34; 13; 12 and 11 individuals in each trap) of the 19 positive emergence traps set in the chicken shed microhabitat. The number of sand flies collected by emergence traps fixed in chicken shed microhabitat varied from 0 to 34 individuals (, SD = 40.87) and no relation with the sampled area of traps was found (r = −0.180, df = 17, p>0.05).
Considering that the number and size of the emergence traps in each microhabitat were not the same, the number of L. longipalpis collected in those traps was transformed into the number of individual per area of sampled microhabitat by the emergence traps (Table 1). Across all microhabitats, 4.42 L. longipalpis were collected per square meter (m2) sampled. For the chicken shed microhabitat, the total density was 17.29 individuals per m2, and for leaf litter under tree microhabitat only 0.26 per m2 (Table 1). Lutzomyia longipalpis was collected in the chicken shed microhabitat almost every month during the study period, but with a peak in August/2005 (Fig. 4A). This month corresponded to intense water deficit period as indicated by the 10-day soil water balance data (Fig. 4C).
Figure 4. Distribution of Lutzomyia longipalpis and extract of the ten–day soil water balance.
Monthly William's average (N) and log of density (fly/m2) of individuals collected in CDC and emergence traps, respectively, from March/2005 to February/2006. A: Promissão and B: Dracena. Water balance data from January/2005 to February/2006, for the municipality of C: Promissão and D: Dracena.doi:10.1371/journal.pntd.0002443.g004
Emergence traps in Dracena.
the leaf litter under tree microhabitat was investigated in all the 18 dwellings (62 sites), the uncovered debris microhabitat in 10 (12 sites) of them, the chicken shed in eight (10 sites), and the kennel microhabitat in only two (two sites).
A total of 576 emergence traps were used to sampled 37.10 m2 of soil: 31.74 m2 in leaf litter and base of tree trunks, 2.45 m2 in chicken sheds, 2.25 m2 in uncovered debris and 0.66 m2 in kennel (Table 2). Only three (16.6%) peridomiciles and four (0.69%) emergence traps were positive for L. longipalpis: one chicken shed, one leaf litter and two uncovered debris microhabitats. Overall, the proportion of positive leaf litter microhabitat was significantly smaller than in uncovered debris and chicken shed microhabitats (χ2 = 58.53, df = 2, p<0.001) but no statistical difference was found between the proportion of positivity of the last two (χ2 = 0.065, df = 1, p>0.05).
Table 2. Summary of Lutzomyia longipalpis catches in soil emergence traps in the municipality of Dracena, from March/2005 to February/2006.doi:10.1371/journal.pntd.0002443.t002
All the 29 sand flies collected in emergence traps in this municipality were L. longipalpis: 14 individuals were collected in chicken sheds (5.71 flies per square meter sampled), four in leaf litter (0.13/m2) and 11 in uncovered debris (4.89/m2). The L. longipalpis male/female ratio was 1:1 in leaf litter under tree microhabitat, 2.5:1 in chicken sheds, and 1.2:1 in uncovered debris (Table 1). The frequency distribution analysis showed that 19 (65.51%) L. longipalpis individuals were collected in only two traps (one trap with 12 individuals in a chicken shed and one trap with seven individuals in uncovered debris). The number of sand flies collected by emergence traps fixed in all microhabitats varied from 0 to 12 individuals (, SD = 15.02) and no relation with the sampled area was found (r = −0.341, df = 4, p>0.05). Lutzomyia longipalpis was only collected in six months of the study period, with a peak in April and May/2005 (Fig. 4B). These months corresponded to moderate or intense water deficit periods kept by sporadic low rainfall index as indicated by the 10-day soil water balance data (Fig. 4D).
Neither in Promissão nor Dracena, were sand flies collected in soil with a high concentration of fresh chicken faeces, such as that found under roosts. The greatest number of sand flies were collected in the areas immediately surrounding the chicken sheds, where the organic matter such as chicken faeces and chicken food is completely degraded, drier, mixed in the soil and probably suitable as a food source for the larvae.
In the same study period, from March 2005 to February 2006, a total of 3,860 (3,070 males and 790 females) and 9,883 L. longipalpis (7,607 males and 2,276 females) were collected in CDC light traps set in the 20 dwellings in Promissão and Dracena, respectively. More than 85% of individuals (3,414 to Promissão and 8,763 to Dracena,) were collected in the peridomicile habitat, principally in CDC traps situated in the chicken shed microhabitat. Lutzomyia longipalpis was collected in every month in both municipalities, with higher abundance during May/2005 and January/2006 in Promissão (Fig. 4A) and during April, May and June in Dracena (Fig. 4B). These months corresponded to moderate water deficit periods kept by sporadic low rainfall index as indicated by the 10-day soil water balance extract (Fig. 4C, 4D).
In Promissão no significant correlation was found between the density of L. longipalpis observed in emergence traps in chicken sheds and CDC trap collections during the study months (r = 0.007, df = 10, p>0.05). On the other hand, this correlation was significantly positive to Dracena, when the density for the three positive microhabitats, chicken shed, leaf litter under tree and uncovered debris, is considered (r = 0.629, df = 10, p<0.05).
In the same emergence traps where L. longipalpis were collected, the following taxonomic groups were collected: Hymenoptera (Formicidae), Coleoptera (adults), and Diptera (Sciaridae, Sphaeroceridae, Mycetophilidae, Phoridae, Fanniidae, Scenopinidae, Cloropidae, Caliphoridae, Lauxaniidae, Dolichopodidae, Milichiidae, Muscidae, Drosophilidae, Cecidomyidae, Sepsidae). In the soil samples obtained adjacent to emergence traps where L. longipalpis was present, adults or larvae from the following taxa were also collected: Psocoptera, Coleoptera, Hymenoptera, Homoptera, Araneidae, Collembola and Diptera (Drosophilidae, Sciaridae, Chironomidae, Phoridae, Sepsidae, and other Nematocera larvae). The Scenopinidae larvae are recognised predators of other Diptera larvae .
Second period of study
Emergence traps in Promissão.
In the second series of collections, from September 2005 to January 2007, a total of 16 L. longipalpis (nine males and seven females) were collected in soil emergence traps. Only one male was collected in one leaf litter under tree microhabitat. One male and one female were collected in chicken shed-2 and 13 individuals (seven males and six females) were collected in chicken shed-1. In this last case, five out of six positive traps were set in adjacent quadrats and the spatial distribution pattern of L. longipalpis immature stages evaluate by the variance mean ratio, (χ2 = 169, df = 71, p<0.001) and the Morisita's Index, Id = 9.230, χ2 = 169, df = 71, p<0.001) indicates a spatial contagious distribution.
The new design of emergence trap used in the present study is easy to construct, very straightforward to install and allows for longer periods between visits to the traps to remove sand flies (every 20 days, approximately).
Clearly, the chicken shed was the microhabitat that contributed the most to the high positivity of investigated dwellings, for the high proportion of positivity of emergence traps and to the increased number of L. longipalpis collected in the municipality of Promissão. In Dracena, chicken sheds were also the most productive microhabitat for L. longipalpis, however with a much lower number of positive emergence traps and sand flies than found in Promissão. Probably, this was due to both the smaller number of chicken shed investigated (about 50% smaller) and sampled area in the chicken shed microhabitat (about three times smaller) than that in Promissão.
Compared to the leaf litter under tree microhabitat, the chicken shed and uncovered debris areas are much smaller, and the population boundaries are readily apparent. This facilitates sampling of the population and estimating its size, particularly if the population shows contagious spatial distribution. In this sense, although in the present study the leaf litter under tree microhabitat has contributed only 5.40% of the total L. longipalpis collected, one should not ignore the possibility of a high production of L. longipalpis in this microhabitat, because this species is by far the most common in the peridomiciles of the studied areas. However, the differences in microhabitat size were most likely not the reason for the great difference observed between the positivity and productivity of these microhabitats. The chicken sheds are probably more favourable breeding sites because they are important blood feeding and resting sites for females of L. longipalpis and the abundance of faeces can provide a source of organic material for larval food.
One advantage of employing emergence traps to detect sand fly breeding sites is that they allow estimates of population densities from the observed productivity of breeding sites, expressed in adults/area/time , . Considering the period of investigation, the number of the traps and the sampled area, the estimated production of L. longipalpis in the chicken shed microhabitat was by far the highest among all the researched microhabitats in the present study. For instance, the density of 17.29 sand flies per m2 or 41.17 sand flies per 100 m2 per day estimate for the total of chicken shed microhabitat in Promissão is a much higher value than found for L. longipalpis and other species in Neotropical region. In emergence traps set near pigsty microhabitats Ferro et al. (1997) estimated a density of 4.96 L. longipalpis per m2 sampled . Considering other sand fly species, Rutledge & Ellenwood (1975) in Panama, and Arias & Freitas (1982), Casanova (2001) and Alencar (2007) in Brazil, estimated from the total of sand flies species captured in the litter of the forest floor, a production of 24.4, 4.1, 24.0 and 5.8 sand flies per 100 m2 per day, respectively , , , . Notably, the 34 L. longipalpis collected in one emergence trap with 20.32 cm of diameter (0.032 m2), set in a chicken shed in Promissão, corresponded to a density of 1,062.5 sand flies per m2. Besides that, the high density of 50.7 sand flies per m2 obtained in chicken shed microhabitats during August 2005 in Promissão allowed to estimate a production of 121.7 L. longipalpis per 100 m2 per day. This high potential production of L. longipalpis agrees with the high abundance of adults in CDC traps set in the peridomicile.
The sexual ratio pro males may be a result of a possible greater male vagility after emergence facilitating their contact with the adhesive paper. Some of the male flies collected in the emergence traps still showed partial or no rotation of their genitalia. However, there is still the necessity to evaluate the efficiency of the traps of different dimensions to collect the emergence population inside them. This can be tested by releasing a known number of pupae or newly emerged adults into the traps, preferably at the central point .
Although the sampling of the microhabitats in the first period of study had been done with an uneven number of traps of different sizes, the intensive sampling, the concentration of a great number of L. longipalpis in few traps and the great number of traps with few or no flies seemed to be a strong indication of its contagious spatial distribution pattern. During the second study period, designed to identify where the concentrations occurred, and how great and how frequent they were, the indices used also showed a contagious distribution pattern. However, the abundance of L. longipalpis was low and new long term experiments, with greater number of traps and an exact positioning in the grid are necessary to allow the use of more accurate methods. These results suggest that females lay their eggs in clutches in restricted microhabitats, as indicated by Ferro et al. (1997) . Probably the female sand fly in this, and other, species has the ability to detect food, shade, humidity and physical-chemical soil constitution, which are generally graded in a habitat. Some studies have shown that female sand flies locate an appropriate site by orienting towards semiochemical oviposition attractant components of eggs and animal faeces . Contagious spatial distribution is the pattern commonly reported for insects in natural environments  and has already been suggested for sand flies species in forest floor microhabitats . The contagious spatial distribution pattern can be responsible for the commonly reported difficulty in finding immature forms in soil samples or adult forms in emergence traps , .
The associated fauna may offer important information on species which might act as a sand fly breeding site indicator. Detecting larvae from a Diptera predator group in the same chicken shed sample where L. longipalpis were found is interesting from the biological control perspective. Collecting E. cortelezzii, E. lenti, E. termithophila and N. neivai in chicken shed together with L. longipalpis also points to the importance that this environment can have in the ecoepidemiology of cutaneous leishmaniasis.
The high abundance of L. longipalpis detected in the CDC traps fixed in peridomicile associated with animal pens, especially chicken sheds, has been frequently observed in several other rural and urban areas of Brazil , –. The lack of correlation between the number of adult sand flies collected in CDC traps and the number of immature stages in soil samples, or adults in emergence traps, is not uncommon , , . This could be due to an accumulation of multiple generations of flies in the CDC trap, in addition to the fact that adults are attracted to the host from an area considerable larger than the emergence microhabitats that are sampled. Probably the combined effects of rainfall, air temperature and evapotranspiration, which determine the soil water balance, influence the quality of the breeding site microhabitats and consequently determine the adult sand fly population fluctuations , , , , . In the present study, moderate water deficit periods seem to be favorable for population increase and periods of high water surplus seem to negatively affect the immature and adult populations of L. longipalpis. For Promissão, the rising curve for adults from October on, which led to a peak in December, may have been influenced by moderated water surplus periods between October and December, which only happened in this municipality and not in Dracena. On the other hand, longer periods of intense draught, like the one which happened more evidently in Dracena (water balance deficit from March to December), may be detrimental to the immature stages. The same has been observed with L. longipalpis and other sand fly species in urban and rural areas of São Paulo state , .
Both the high proportion of positive sites and high density of individuals in emergence traps indicate that the chicken shed microhabitat is the preferential breeding site of L. longipalpis in the study areas. In this sense, a new item – act as breeding sites to L. longipalpis - can be added to list of Alexander et al (2002) as factor associated to chicken sheds that increases the risk of transmission of L. (L.) i. chagasi to humans in Brazil . Raising chickens is relatively common and both culturally and socio-economically important in urban, peri-urban and rural areas of the municipalities in all regions of Brazil. Its importance in the ecoepidemiology of zoonotic visceral involves some type of balance between zooprophylaxis, maintenance of sand fly populations and attraction of reservoir hosts . Spatial analysis studies to detect areas at increased risk for visceral leishmaniasis such as those developed by Cerbino-Neto et al (2009), could evaluate the presence of chicken in the neighbourhood as an environmental factor associated with incidence of the disease . It would be of interest to evaluate the efficiency of vector control interventions, such as environmental change and insecticide (chemical or biological), in chicken shed microhabitats, with the aim of reducing the adult population. The abundance of the female population size is a critical parameter of vectorial capacity , .
If the chicken shed, as a preferential breeding site, has an important role in determining the population abundance of adult forms of L. longipalpis, control strategies aimed at immature stage population in this microhabitat could be cheap, practical, and effective at reducing the populational abundance of females and consequently, the transmission rate. Furthermore, the efficacy of residual insecticide and others potential control strategies against adult forms - i.e. impregnated dog-collar , , zooprophylaxis  synthetic sand fly pheromone in conjunction whit insecticide ,  – could be improved through of the control of immature stages of L. longipalpis population in chicken shed microhabitats.
The authors thank Dr. Prof. Jeffrey Jon Shaw for comments, suggestions and reviewing this manuscript.
Conceived and designed the experiments: CC. Performed the experiments: CC MTMA SMPS MLGM APP. Analyzed the data: CC FECJ APP. Contributed reagents/materials/analysis tools: CC MTMA SMPS. Wrote the paper: CC MTMA SMPS MLGM FECJ APP.
- 1. Lainson R, Rangel EF (2005) Lutzomyia longipalpis and the eco-epidemiology of American visceral leishmaniasis, with a particular reference to Brazil: A review. Mem Inst Oswaldo Cruz 100: 811–827. doi: 10.1590/s0074-02762005000800001
- 2. Shaw JJ (2006) Further thoughts on the use of the name Leishmania (Leishmania) infantum chagasi for the aetiological agent of American visceral leishmaniasis. Mem Inst Oswaldo Cruz 101: 577–579. doi: 10.1590/s0074-02762006000500017
- 3. Bauzer LGSR, Souza NAS, Maingon RDC, Peixoto AA (2007) Lutzomyia longipalpis in Brazil: a complex or a single species? A mini-review. Mem Inst Oswaldo Cruz 102: 1–12. doi: 10.1590/s0074-02762007000100001
- 4. Desjeux P (2004) Leishmaniasis: current situation and new perspectives. Comp Immunol Microbiol Infect Dis 27: 305–318. doi: 10.1016/j.cimid.2004.03.004
- 5. Ministry of Health of Brazil (2006) Manual de vigilância e controle da leishmaniose visceral. 3rd: 1–122.
- 6. Maia-Elkhoury ANS, Alves WA, Sousa-Gomes ML, Sena JM, Luna EA (2008) Visceral leishmaniasis in Brazil: trends and challenges. Cad Saúde Pública 24: 2941–2947. doi: 10.1590/s0102-311x2008001200024
- 7. Werneck GL (2008) Forum: Geographic spread and urbanization of visceral leishmaniasis in Brazil. Introduction. Cad Saúde Pública 24: 2937–2940. doi: 10.1590/s0102-311x2008001200023
- 8. Secretary of Health of São Paulo State (2010) Classificação epidemiológica dos municípios segundo o Programa de Vigilância e Controle da Leishmaniose Visceral Americana no estado de São Paulo, atualizado em maio de 2010. BEPA 7 (77)
- 9. Ministry of Health of Brazil (2012) Casos confirmados de Leishmaniose Visceral, Brasil, Grandes Regiões e Unidades Federadas. 1990 a 2011. Available: http://portal.saude.gov.br/portal/arquivos/pdf/2012_11_casos_de_lv_entre_1990_e_2011_final.pdf. Accessed 11 December 2012.
- 10. Secretary of Health of São Paulo State (2012) Dados estatísticos da Leishmaniose Visceral Americana de 1999 a 2012. Available from: http://www.cve.saude.sp.gov.br/htm/zoo/leishv_dados.html. Accessed 11 December 2012.
- 11. Secretary of Health of São Paulo State (2006) Manual de Vigilância e Controle da Leishmaniose Visceral Americana do Estado de São Paulo. 158 p.
- 12. Romero GAS, Boelaert M (2011) Control of visceral leishmaniasis in Latin America – A systematic review. PLoS Negl Trop Dis 4 (1) e584. doi: 10.1371/journal.pntd.0000584
- 13. Dye C (1996) The logic of visceral leishmaniasis control. Am J Trop Med Hyg 55: 125–130.
- 14. Costa CHN, Tapety CMM, Werneck GL (2007) Controle da leishmaniose visceral em meio urbano: estudo de intervenção randomizado fatorial. Rev Soc Bras Med Trop 40: 415–419. doi: 10.1590/s0037-86822007000400009
- 15. Silva JP, Werneck GL, Macedo EC, Carvalho H, Cruz MSP (2012) Factors associated with Leishmania chagasi infection in domestic dogs from Terezina, State of Piauí, Brazil. Rev Soc Med Trop 45: 480–484. doi: 10.1590/s0037-86822012005000009
- 16. Courtenay O, Quinnell RJ, Garcez LM, Shaw JJ, Dye C (2002) Infectiousness in a cohort of Brazilian dogs: why culling fails to control visceral leishmaniasis in areas of high transmission. J Infect Dis 186: 1314–1320. doi: 10.1086/344312
- 17. Reithinger R, Coleman PG, Alexander B, Vieira EP, Assis G, et al. (2004) Are insecticide-impregnated dog collars a feasible alternative to dog culling as a strategy for controlling canine visceral leishmaniasis in Brazil? Int J Parasitol 34: 55–62. doi: 10.1016/j.ijpara.2003.09.006
- 18. Costa CHN (2011) How effective is dog culling in controlling zoonotic visceral leishmaniasis? A critical evaluation of the science, politics and ethics behind this public health policy. Rev Soc Bras Med Trop 44: 232–242. doi: 10.1590/s0037-86822011005000014
- 19. Alexander B, Maroli M (2003) Control of phlebotomine sandflies. Med Vet Entomol 17: 1–18. doi: 10.1046/j.1365-2915.2003.00420.x
- 20. Amóra SSA, Bevilaqua CML, Feijó FMC, Alves ND, Maciel MV (2009) Control of Phlebotomine (Diptera: Psychodidae) leishmaniasis vectors. Neotrop Entomol 38: 303–310. doi: 10.1590/s1519-566x2009000300001
- 21. Quinnell RJ, Dye C (1994) An experimental study of the peridomestic distribution of Lutzomyia longipalpis (Diptera: Psychodidae). Bull Entomol Res 84: 379–382. doi: 10.1017/s0007485300032508
- 22. Alexander B, de Carvalho RL, MacCallum H, Pereira MH (2002) Role of the domestic chicken (Gallus gallus) in the epidemiology of urban visceral leishmaniasis in Brazil. Em Inf Dis 8: 1480–1485. doi: 10.3201/eid0812.010485
- 23. Rangel EF, Vilela ML (2008) Lutzomyia longipalpis (Diptera, Psychodidae, Phlebotominae) and urbanization of visceral leishmaniasis in Brazil. Cad Saúde Pública 24: 2948–2952. doi: 10.1590/s0102-311x2008001200025
- 24. Dye C, Davies CR, Lainson R (1991) Communication among phlebotomine sand flies: a field study of domesticated Lutzomyia longipalpis population in Amazonian Brazil. Anim Behav 42: 183–192. doi: 10.1016/s0003-3472(05)80549-4
- 25. Morrison AC, Ferro C, Morales A, Tesh RB, Wilson ML (1993) Dispersal of the sand fly Lutzomyia longipalpis (Diptera: Psychodidae) at an endemic focus of visceral leishmaniasis in Colombia. J Med Entomol 30: 427–35.
- 26. Ferro C, Pardo R, Torres M, Morrison A (1997) Larval microhabitats of Lutzomyia longipalpis (Diptera: Psychodidae) in an endemic focus of visceral leishmaniasis in Colombia. J Med Entomol 34: 719–728.
- 27. Feliciangeli MD (2004) Natural breeding places of phlebotomine sandflies. Med Vet Entomol 18: 71–80. doi: 10.1111/j.0269-283x.2004.0487.x
- 28. Warburg A, Faiman R (2011) Research priorities for the control of phlebotomine sand flies. J Vector Ecol 36 Suppl1: S10–16. doi: 10.1111/j.1948-7134.2011.00107.x
- 29. Rutledge LC, Ellenwood DA (1975) Production of phlebotomine sandflies on the open forest floor in Panama: the species complement. Environ Entomol 4: 71–77.
- 30. Maroli M, Feliciangeli MD, Arias J (1997) Metodos de Captura, Conservacion y Montaje de los Flebotomos (Diptera: Psychodidae). Organización Panamericana de la Salud, Oficina Sanitaria Panamericana, Oficina Regional de la Organización Mundial de la Salud. Washington, D.C. 72 p.
- 31. Killick-Kendrick R (1999) The biology and control of phlebotomine sand flies,. Clinics in dermatology 17: 279–289. doi: 10.1016/s0738-081x(99)00046-2
- 32. Brazil RP, Brazil BG (2003) Biologia de flebotomíneos neotropicais. In: Rangel EF, Lainson R, editors. Flebotomíneos do Brasil. Rio de Janeiro: Editora Fiocruz. pp. 257–274.
- 33. Arias JR, Freitas RA (1982) On the vectors of cutaneous leishmaniasis in the Central Amazon of Brazil. 4. Sand fly emergence from a “terra firme” forest floor. Acta Amazon 12: 609–611.
- 34. Bettini S, Contini C, Atzeni MC, Tocco G (1986) Leishmaniasis in Sardinia. I. Observations on a larval breeding site of Phlebotomus perniciosus, Phlebotomus perfiliewi perfiliewi and Sergentomyia minuta (Diptera: Psychodidae) in the canine leishmaniasis focus of Soleminis (Cagliari). Ann Trop Med Parasitol 80: 307–315.
- 35. Casanova C (2001) A soil emergence trap for collection of phlebotomine sand flies. Mem Inst Oswaldo Cruz 96: 273–275. doi: 10.1590/s0074-02762001000200023
- 36. Alencar RB (2007) Emergência de flebotomíneos (Diptera: Psychodidae) em chão de floresta de terra firme na Amazônia Central do Brasil: Uso de um modelo modificado de armadilha de emergência. Acta Amazon 37: 287–292. doi: 10.1590/s0044-59672007000200016
- 37. Moncaz A, Faiman R, Kirstein O, Warburg A (2012) Breeding sites of Phlebotomus sergenti, the sand fly vector of cutaneous leishmaniasis in the Judean Desert. PLoS Negl Trop Dis 6 (7) e1725. doi: 10.1371/journal.pntd.0001725
- 38. Centro de Pesquisas Meteorológicas e Climáticas Aplicadas à Agricultura (2012) Clima dos Municípios Paulistas: a classificação climática de Koppen para o Estado de São Paulo. Avaiable: http://www.cpa.unicamp.br/outras-informacoes/clima-dos-municipios-paulistas.html. Accessed 11 December 2012.
- 39. Galati EAB (2003) Classificação de Phlebotominae. In: Rangel EF, Lainson R, editors. Flebotomíneos no Brasil. Rio de Janeiro: Editora Fiocruz. pp. 23–52.
- 40. Thornthwaite CW, Mather JR (1955) The water balance. Centerton: Publications in climatology, Drexel Institute of Technology Laboratory of Climatology. 104 p.
- 41. Centro Integrado de Informações Agrometeorológicas (2012) CIIAGRO on line. Updated Oct, 2012. Avaiable: http://www.ciiagro.sp.gov.br/ciiagroonline/MenuMonClim.htm. Accessed 11 December 2012.
- 42. Brower JE, Zar JH (1984) Field & laboratory methods for general ecology. Dubuque: WCB. 226 p.
- 43. Hadow AJ (1960) Studies on the biting-habits and medical importance of East African mosquitoes in the genus Aedes. I. Subgenera Aedimorphus, Banksinella and Nunnius. Bull Entomol Res 50: 759–779. doi: 10.1017/s000748530005478x
- 44. Kelsey LP (1969) A revision of the Scenopinidae (Diptera) of the world. Bull of the United States National Museum 277: 1–336.
- 45. Southwood TRE (1987) Ecological methods with particular reference to the study of insect populations. London: Methuen. 524 p.
- 46. Dougherty MJ, Guerin PM, Ward RD (1995) Identification on oviposition attractants for the sandfly Lutzomyia longipalpis (Diptera: Psychodidae) in volatiles of faeces from vertebrates. Physiol Entomol 2: 23–32. doi: 10.1111/j.1365-3032.1995.tb00797.x
- 47. Bray DP, Alves GB, Dorsal ME, Brazil RP, Hamilton JGC (2010) Synthetic sex pheromone attracts the leishmaniasis vector Lutzomyia longipalpis to experimental chicken sheds treated with insecticide. Parasites and Vectors 3: 16. doi: 10.1186/1756-3305-3-16
- 48. Colla-Jacques FE, Casanova C, Prado AP (2010) Study of sand fly fauna in an endemic area of American cutaneous leishmaniasis and canine visceral leishmaniasis in the municipality of Espírito Santo do Pinhal, São Paulo, Brazil. Mem Inst Oswaldo Cruz 105: 208–215. doi: 10.1590/s0074-02762010000200017
- 49. Jeraldo VLS, Góes MAO, Casanova C, Melo CM, Araújo ED, et al. (2012) Sandfly fauna in an area endemic for visceral leishmaniasis in Aracaju, State of Sergipe, Northeast Brazil. Rev Soc Bras Med Trop 45: 318–322. doi: 10.1590/s0037-86822012000300008
- 50. Hanson WJ (1961) The breeding places of Phlebotomus in Panama (Diptera: Psychodidae). Ann Entomol Soc Am 54: 317–322.
- 51. Sangiorgi B, Miranda DN, Oliveira DF, Santos EP, Gomes FR, et al. (2012) Natural breeding places for phlebotomine sand flies (Diptera: Psychodidae) in a semiarid region of Bahia state, Brazil. J Trop Med 1–5. doi: 10.1155/2012/124068
- 52. Morrison AC, Ferro C, Pardo R, Torres M, Wilson ML, et al. (1995) Seasonal abundance of the sand fly Lutzomyia longipalpis (Diptera: Psychodidae) at an endemic focus of visceral leishmaniasis in Colômbia. J Med Entol 32: 538–548.
- 53. Casanova C, Natal D, Santos FAM (2009) Survival, population size, and gonotrophic cycle duration of Nyssomyia neivai (Diptera: Psychodidae) at an endemic area of American Cutaneous Leishmaniasis in Southeastern Brazil. J Med Entomol 46: 42–50. doi: 10.1603/033.046.0106
- 54. Cerbino-Neto J, Wernecket GL, Costa CHN (2009) Factors associated with the incidence of urban visceral leishmaniasis: an ecological study in Terezina, Piauí state, Brazil. Cad Saúde Pública 25: 1543–1551. doi: 10.1590/s0102-311x2009000700012
- 55. Reisen WK (1989) Estimation of vectorial capacity relationship to disease transmission by malaria and arbovirus vectors. Bull. Soc. Vector Ecol 14: 39–40.
- 56. Killick-Kendrick R, Killick-Kendrick M, Focheux C, Dereure J, Puech MP, et al. (1997) Protection of dogs from bites of phlebotomine sandflies by deltamethrin collars for control of canine leishmaniasis. Med Vet Entomol 11: 105–111. doi: 10.1111/j.1365-2915.1997.tb00298.x
- 57. Ward RD, Morton IE, Brazil RP, Trumper S, Falcão AL (1990) Preliminary laboratory and field trials of a heated pheromone trap for the sandfly Lutzomyia longipalpis (Diptera: Psychodidae). Mem Inst Oswaldo Cruz 85: 445–452. doi: 10.1590/s0074-02761990000400009