Anti-leishmanial drug regimens that include a single dose AmBisome® could be suitable for eastern African patients with symptomatic visceral leishmaniasis (VL) but the appropriate single dose is unknown.
A multi-centre, open-label, non-inferiority, randomized controlled trial with an adaptive design, was conducted to compare the efficacy and safety of a single dose and multiple doses of AmBisome® for the treatment of VL in eastern Africa. The primary efficacy endpoint was definitive cure (DC) at 6 months. Symptomatic patients with parasitologically-confirmed, non-severe VL, received a single dose of AmBisome® 7.5 mg/kg body weight or multiple doses, 7 times 3 mg/kg on days 1–5, 14, and 21. If interim analyses, evaluated 30 days after the start of treatment following 40 or 80 patients, showed the single dose gave significantly poorer parasite clearance than multiple doses at the 5% significance level, the single dose was increased by 2·5 mg/kg. In a sub-set of patients, parasite clearance was measured by quantitative reverse transcriptase (qRT) PCR.
The trial was terminated after the third interim analysis because of low efficacy of both regimens. Based on the intention-to-treat population, DC was 85% (95%CI 73–93%), 40% (95%CI 19–64%), and 58% (95%CI 41–73%) in patients treated with multiple doses (n = 63), and single doses of 7·5 (n = 21) or 10 mg/kg (n = 40), respectively. qRT-PCR suggested superior parasite clearance with multiple doses as early as day 3. Safety data accorded with the drug label.
The tested AmBisome® regimens would not be suitable for VL treatment across eastern Africa. An optimal single dose regimen was not identified.
Visceral leishmaniasis is a potentially fatal disease which affects 0.2–0.4 million people every year, principally in South-East Asia, Latin America or Eastern Africa. Currently the safest drug in use is AmBisome®, which cures 90% of patients in India at 5 mg/kg, and is even more effective at higher doses (10 mg/kg) or in combination with miltefosine or paromomycin. These regimens have been shown to be equally cost-effective in India. However, the drug requires a cold chain for storage and reconstitution prior to injection. Although it is licensed for use in eastern Africa, in practice it is mainly used as a second-line treatment. A small study carried out in Kenya indicated that a higher dose is necessary in eastern Africa in contrast to Asia. This study aimed to determine the minimum single dose that is safe and effective for treatment of eastern African VL patients so as to be used in simplified treatment regimens. However, the tested regimens were found to be ineffective, and an optimal single dose that could potentially be used in simplified treatment regimens was not identified.
Citation: Khalil EAG, Weldegebreal T, Younis BM, Omollo R, Musa AM, et al. (2014) Safety and Efficacy of Single Dose versus Multiple Doses of AmBisome® for Treatment of Visceral Leishmaniasis in Eastern Africa: A Randomised Trial. PLoS Negl Trop Dis 8(1): e2613. doi:10.1371/journal.pntd.0002613
Editor: Elodie Ghedin, University of Pittsburgh, United States of America
Received: April 14, 2013; Accepted: November 18, 2013; Published: January 16, 2014
Copyright: © 2014 Khalil et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: DNDi, the sponsor of the study, was involved in the final stages of study protocol development and data analysis; and also supported the preparation of the draft manuscript by a medical writer. The decision to publish was made by investigators as well as the sponsors. DNDi acquired funds for the conduct of the trial from: Department for International Development (DFID), UK; Médecins Sans Frontières/Doctors without Borders; International; Medicor Foundation, Liechtenstein; Ministry of Foreign and European Affairs (MAEE), France; Spanish Agency for International Development Cooperation (AECID), Spain; Swiss Agency for Development and Cooperation (SDC), Switzerland; private foundations and individual donors.
Competing interests: The authors have declared that no competing interests exist. Gilead Sciences, USA, provided the investigational product, AmBisome®. Gilead Sciences and the sponsors, DNDi, have professional collaborations. This collaboration does not alter our adherence to all PLOS NTDs policies on sharing data and materials.
Visceral leishmaniasis (VL) is a life-threatening disease and a major health burden in developing countries , , . WHO estimates there are approximately 0.2–0.4 million cases of VL annually; and more than 90% of global VL cases occur in six countries: India, Bangladesh, Sudan, South Sudan, Ethiopia and Brazil . In eastern Africa approximately 30,000 people develop symptomatic VL and 4,000 die every year , .
For decades, the mainstay of VL treatment in eastern Africa has been antimonials such as sodium stibogluconate (SSG), but this treatment is cardiotoxic  and requires a 4-week hospitalisation imposing a huge economic burden on families . Monotherapy with intramuscular paromomycin (PM) for 3 weeks was shown to be less efficacious in eastern Africa  than in Asia , but a 17-day treatment with a combination of SSG and PM showed good efficacy and is now recommended as first-line treatment by WHO. However, this treatment also requires a relatively long treatment course and twice daily injections . Currently, the safest anti-leishmanial drug is AmBisome®, a liposomal amphotericin B formulation with significantly diminished renal toxicity . In trials in India, cure rates of around 90% were obtained with single AmBisome® doses of 5 mg/kg . In addition, 95% efficacy was achieved with higher single doses (10 mg/kg) or when used in combination with miltefosine or paromomycin . Although licensed and recommended for first-line treatment of VL in immunocompetent patients , Ambisome® use in eastern Africa has been mostly limited to second line treatment in a few centres mainly due to its high cost and cold storage requirements . A small study with AmBisome® conducted in Kenya indicated higher doses were required than had been used in studies in India. Doses of 2 mg/kg given 3, 5, or 7 times to groups of 10 patients resulted in cure rates of 20%, 90%, and 100% respectively .
The aim of this study was to determine the minimum efficacious and safe single dose for the likely future use of the drug as part of a shorter course of treatment regimen for eastern African VL patients. The trial was undertaken with the goal of ultimately identifying a short and simplified treatment regimen that includes AmBisome®. Such a regimen will improve patient compliance and will have the advantage of a reduced cost.
The study design and protocol have been published, in compliance with CONSORT requirements . The protocol was approved in Ethiopia, Sudan and UK by the authors' Institutional and National Ethics Committees. These committees were: the Research Ethics Institutional Review Board of the Faculty of Medicine – Addis Ababa University; The Institutional Review Board of the University of Gondar; the National Ethics Review Committee (NERC) at the Ethiopian Science & Technology Commission (ESTC); the Health Research Ethics Committee of the Institute of Endemic Diseases (University of Khartoum) and the Directorate of Health Research in the Federal Ministry of Health (Sudan); and the Ethics Committee of the London School of Hygiene and Tropical Medicine. The study was conducted in accordance with the declaration of Helsinki, ICH GCP guidelines, and all applicable legal requirements. All study subjects participated in the study voluntarily, and signed a written ‘Informed Consent Form’ (ICF). The parents or guardians of study participants under the age of 18 years provided written informed consent on their behalf. In addition, minors (age 12–17 years) signed a written assent form. The study was registered at www.clinicaltrials.gov (Clinical Trials Registration number NCT00832208) prior to trial initiation and patient recruitment.
The study was designed as a multi-centre, open-label, non-inferiority, randomized controlled trial, using a sequential-step design to evaluate the efficacy and safety of a single dose treatment regimen of intravenous AmBisome® (either 7.5, 10.0, 12.5 or 15.0 mg/kg body weight) compared to the reference multiple dose regimen currently approved in the USA: 3 mg/kg body weight on days 1 to 5, 14, and 21. The single dose tested in the first cohort was 7·5 mg/kg body weight (figure 1, the consort flowchart). Two interim analyses were planned, after enrolment of 20 and 40 patients per arm, for early detection of inefficacious single doses, based on parasite clearance at day 30 (figure 2) and/or worsening clinical conditions. If the stopping rule was met, the single dose was increased by 2·5 mg/kg and recruitment into the two arms restarted. The multiple dose treatment remained the same throughout. Non-responders to treatment were considered treatment failures and received rescue medication (multiple dose AmBisome® regimen for single-dose failures and SSG for multiple dose failures).
Figure 1. Patient flow chart.
Consort Patient flow chart: AmBisome® multi dose vs. single dose, ITT Intention to treat, PP per protocol, LTFU Lost to Follow-up.doi:10.1371/journal.pntd.0002613.g001
Figure 2. Summary of study design.
Interim analyses were based on day 30 cure in the Intention-to-Treat (ITT) population. iv intravenous, mg/kg/day milligram/kilogram body weight/day.doi:10.1371/journal.pntd.0002613.g002
Patients with age of at least 4 years, confirmed HIV-negative, parasitologically-confirmed non-severe VL, were enrolled in three centres: (1) Gondar University Hospital, Amhara Regional State, Northern Ethiopia; (2) Arba Minch Hospital, Gamo Gofa Zone, Southern Nations, Nationalities and Peoples Regional State, Southern Ethiopia; and per protocol amendment (3) Ministry of Health Hospital, Kassab, Gedaref State, Eastern Sudan from May 2009 to September 2010. Exclusion criteria were signs/symptoms of severe VL (patients who were very weak, unable to walk, bleeding, jaundiced, suffering from sepsis and other concomitant infections/illnesses); anti-leishmanial or unlicensed investigational treatments within six months; underlying chronic disease such as severe cardiac, pulmonary, renal, or hepatic impairment; serum creatinine outside the normal range; liver function tests more than 3 times the normal range; platelet count less than 40,000/mm3; known alcohol abuse; pregnancy or lactation; concomitant acute drug usage for malaria and bacterial infection; pneumonia within last 7 days; known hypersensitivity to AmBisome® or amphotericin B; any other condition which may invalidate the trial.
On pre-specified treatment days, AmBisome® dosage was calculated according to body weight. Preparation included reconstitution with sterile water for injection and filtration according to the manufacturer's instructions. Administration was by slow intravenous infusion in 5% dextrose solution. During infusion, patients were closely observed with regular monitoring of vital signs (blood pressure and pulse). A test dose was administered in the first 10 minutes of infusion, and the patients carefully observed for 30 minutes. The time of treatment and dosage was recorded.
Randomisation and blinding
Patients were randomized to receive either treatment using a computer-generated randomisation list, stratified by site. Individual treatment allocations were placed in sealed, opaque envelopes which were opened after a patient had been entered into the trial. It was not possible to blind patients or treating physicians due to the nature of the intervention.
Primary (definitive/final cure) and secondary (initial cure) efficacy endpoints were determined by parasitological assessment, as the most objective and consistent method across treatment centres at six months follow-up and at Day 30, respectively. Day 30 was considered as the end of treatment time point for initial cure assessment. Definitive cure was defined as absence of parasites in tissue aspirates (bone marrow, lymph node or spleen) with no relapse of signs and symptoms of VL during six months follow-up. Initial cure was defined on day 30 by absence of parasites in tissue aspirates. For patients with detectable parasites at initial cure assessment, clinical and biological assessments were used, in addition to parasitological results, to ascertain the need for rescue treatment at the end of study treatment at the discretion of the treating clinician, according to the protocol. Patients with a presence of parasites at initial assessment of cure but who were clinically well were invited to return after one month to further assess their status and need for rescue treatment. Patients who did not clear parasites at end of treatment but did by six months follow-up, in the absence of a requirement for additional rescue treatment, were classified as treatment successes at six months as patients who were slow responders, in line with previous trials in the region . Assessment of safety involved monitoring vital signs, documentation of patients' complaints about the treatments, and haematological and biochemical measurements evaluated on days 2 to 5, 7, 14, 21 and 30, and at 3 and 6 months. Treatment emergent adverse events (TEAE) were classified according to the Medical Dictionary for Regulatory Activities (MedDRA). Treatment emergent events were those with onset between day 1 of treatment and day 60 inclusive.
Peripheral Blood Parasitaemia: In Kassab, per protocol amendment, peripheral blood samples were analysed for parasite load in a subset of 5 consenting patients in each of the 10 mg/kg single dose and multiple dose arms. For this, a validated quantitative reverse-transcriptase polymerase-chain-reaction (qRT-PCR) method targeting Leishmania 18S ribosomal RNA was used . Genetic material was extracted using a modified Boom-method , , . qRT-PCR analysis using a Bio-Rad CFX-96 real-time machine (Bio-Rad, Veenendaal, the Netherlands) was performed at Koninklijk Instituut voor de Tropen (KIT).
For the primary endpoint comparison, 120 patients per arm would provide 80% power to detect non-inferiority within a margin of 10%, assuming 95% cure in the reference arm, a one-sided alpha of 0·05 and 15% loss-to-follow-up. In interim analyses, 20 patients per arm would provide 90% power to detect a difference of at least 35% in parasite clearance rate at day 30, assuming 95% cure in the reference arm and a two-sided alpha of 0.05. With 40 patients per arm, there would be 90% power to detect a difference of at least 25% under the same assumptions.
Interim analyses were based on day 30 cure in the Intention-to-Treat (ITT) population. Decision-making at each interim analysis was based on a test of difference between the parasite clearance rates in the single dose arm and multiple dose arms. If the single dose arm showed significantly poorer efficacy (P<0.05), the single-dose was increased prior to re-starting recruitment. Patients allocated to the multiple-dose arm for discontinued single-dose comparisons were not included in comparisons of higher single doses.
Key assumptions for the planned final analysis were not met due to low efficacy in the multi-dose arm and the trial was terminated prematurely (see Results). Cumulative data for each treatment regimen were used to calculate the percentage of patients cured, with exact binomial 95% confidence intervals (CI), at day 30 and 6 months follow-up in ITT and per-protocol (PP) analysis populations. Patients with missing outcome data were excluded from analyses. For safety, the number and percentage of patients per arm experiencing adverse events (AEs) were summarised, for AEs with cumulative incidence higher than 10%. For parasite clearance from peripheral blood, a linear mixed effects regression model using the natural log-transformed parasite loads was applied to estimate the time to clear 50% and 90% of parasites for each individual. Model performance and significance were assessed by analysis of variance (ANOVA).
The most commonly presenting VL symptoms were fever and weight loss, followed by loss of appetite, abdominal swelling, and cough; less commonly observed were epistaxis, diarrhoea, and skin lesions. Other characteristics of patients at entry to the trial are summarized in Table 1. Overall, 82% of patients were male and about half were children. About two thirds of patients were underweight or severely underweight. Mean haemoglobin concentrations were <8·0 g/dl, and anaemia was common, but neither baseline laboratory parameters nor vital signs suggested any major difference among dose groups. Baseline characteristics were generally comparable in the multiple and the 10 mg/kg single dose group, whereas the smaller 7·5 mg/kg dose group showed some imbalances: on average, patients in this group were older and accordingly had higher body weight and larger spleen size, but also had the highest baseline parasitaemia. Patients were younger in Kassab (10·5±5·0 years) than in Gondar (20·9±6·3) or Arba Minch (17·4±10·2 in) and more often female in Kassab (33·3%) than in Gondar (10·5%) or Arba Minch (12%). In Arba Minch, they were more often normal weight (42·0%) than in Gondar (21·1%) or Kassab (28·6%).
Table 1. Baseline data on patient demographics, clinical characteristics and laboratory values.doi:10.1371/journal.pntd.0002613.t001
In the first section of Table 2, parasite clearance rates at day 30 are shown for the three interim analyses. Summary data for the parasite clearance rate at day 30 and the cure rate at 6 months are shown for all patients and for those treated at each of the 3 centres. The IC and DC rates with the standard multiple dose treatment were both 85%. IC rates with single doses of 7·5 and 10 mg/kg were 50% and 73%, respectively; and DC rates were lower, at 40% and 58%, respectively. However, there were variations in treatment response between treatment centres, with poor efficacy in Kassab and Gondar, particularly with single doses. By contrast, at Arba Minch, the multiple doses as well as the single dose of 10 mg/kg resulted in complete cure, and treatment failures were observed with the 7·5 mg/kg dose only. All non-responders were cured after receiving rescue medication.
Table 2. Interim analyses and non-comparative efficacy analysis for primary (day 210) and secondary (day 30) end points.doi:10.1371/journal.pntd.0002613.t002
For the first interim analysis, comparing the 7·5 mg/kg single dose to the multiple dose regimen with 20 and 18 patients per arm, respectively, in the two Ethiopian sites, the stopping rule was met (Table 2: Fisher's exact test, p = 0·015). The single dose was increased to 10 mg/kg, and recruitment restarted at both Ethiopian sites and in an additional site, Kassab, Sudan. There was no significant difference in efficacy found at the next interim analysis comparing 10 mg/kg to the multiple dose arm (p = 0·748), but when 44 patients had been recruited into the multiple dose and 40 patients into the 10 mg/kg single-dose arm, the third interim analysis indicated unexpectedly low initial cure rates in both arms; 84% in the multiple dose and 73% in the single-dose arm. The stopping rule was not met (chi-squared test, p = 0.196), but based on the observed poor efficacy overall, and following discussions with the Data Safety and Monitoring Board (DSMB) and investigators, the sponsor terminated the trial.
At that time, a total of 124 patients had been enrolled into the trial; 63 had received the multiple dose regimen, 20 patients received a single dose of 7·5 mg/kg dose and 41 patients received a single dose of 10 mg/kg (figure 1).
TEAE were common regardless of dose regimen. Severity was mostly mild or moderate and only about 2% of TEAE were rated severe, mostly with respect to laboratory measurements. There was one non-fatal SAE, a pneumonia deemed unlikely related to the drug, and one death due to snakebite. AEs for which relatedness could not be excluded are listed in Table 3. These potential adverse drug reactions were seen in all the three dose groups and occurred in both the multiple and the higher single dose groups with similar frequencies.
Table 3. Infusion-related and drug-related treatment emergent AEs.doi:10.1371/journal.pntd.0002613.t003
At baseline, semi-quantitative microscopy counts on bone marrow aspirates correlated well with parasite loads in peripheral blood when assessed by qRT-PCR (R2: 0·77, p<0·01). Mean natural log-normalized parasite loads (P) were comparable in the single and multiple dose groups (6·4 lnP/mL, 95%CI: 4·6–8·2 versus 5·1 lnP/mL, 95%CI: 3·3–6·8; p = 0·358) at baseline. Three out of the 5 patients of each group had baseline blood parasite loads >50 per mL and these had clearance rates assessed and modelled over the first 7 days. Mean parasite clearance rates were significantly different between the single and the multiple dose group (0·35 per day, 95%CI: 0·00–0·70 versus 1·14 per day, 95%CI: 0·78–1·50; p = 0·012) as early as day 3 (Figure 3), corresponding to mean parasite elimination half-lives of 1·97 days (95% CI: 0·99–278) for the single-dose group and 0·61 days (95%CI: 0·46–0·89) for the multiple dose group. Time required for 90% parasite clearance for single-dose and multiple-dose groups was estimated at 6·55 (95% CI: 3·29–923) and 2·02 (95% CI: 1·53–2·95) days, respectively. One patient in the single dose group had a low blood parasite load at baseline, which increased until day 30, but was no longer detectable after rescue treatment.
Figure 3. Parasite clearance from peripheral blood.
Comparison of parasite clearance rates from peripheral blood in single (10 mg/kg) and multiple dose (7×3 mg/kg) regimens of AmBisome®. Data are from 5 consenting patients in each of the 10 mg/kg single-dose and multiple-dose arms.doi:10.1371/journal.pntd.0002613.g003
We investigated the efficacy of AmBisome® given as multiple or single dose regimens for treatment of VL in eastern Africa. The aim was to determine the minimum efficacious dose and safety of treatments in HIV-uninfected patients. However, the study had to be prematurely terminated due to unacceptably low efficacy in both the single and multiple dose treatment arms, with a cure rate of only 85% in the multiple-dose arm. Adverse effects of treatment in this study were in line with the current drug label.
The overall low efficacy was unexpected, as total doses of 10 mg/kg and above resulted in DC rates of at least 90% in a trial in Kenya . The trial was not powered for data analysis by geographical location (centre) and the results may have been due to chance, but both the 10 mg/kg single dose and 21 mg/kg multiple dose regimens appeared to work very well in the small number of patients treated in Arba Minch Hospital (southern Ethiopia). We have little explanation for the overall poor response seen in this study or for the observed geographical variations. Previously, similar geographical variation in treatment response in these three sites was seen for daily doses of 11 mg/kg body weight paromomycin base over 21 days , a regimen which had also proven efficacious in India . Methodological bias is unlikely in this randomized trial, but differences in base line patient characteristics between the three trial sites could have possibly introduced bias, leading to variation in treatment response. In Arba Minch, about 16% of patients were severely underweight, as compared to more than twice as many in the two sites with poorer responses. However, in all sites, between 58% and 79% of patients were underweight or severely underweight. As poor treatment response was not restricted to the severely underweight patients only, mechanisms are likely to be more complex. Furthermore, the single dose trial in India did not have stricter inclusion/exclusion criteria (it included patients with severe malnutrition) and had a population of patients comparable with ours in terms of mean age and body weight . It is possible that other factors, such as haemoglobin and immunity, may play a role; however, it is unlikely that they can account for the large differences seen between India and eastern Africa or within Africa. The poor response may relate to characteristics of either the host or the parasite or a combination of both. Of note, the disease ecology in Kenya and southern Ethiopia is similar in terms of transmission cycle, as is also the case between the north Ethiopia and eastern Sudan VL . Regarding the parasite; susceptibility to treatments (e.g. SSG) is known to be different in Africa and India, although development of resistance is unlikely to account for the poor response in this study. In Africa, resistance has generally not been an issue and amphotericin B has not been widely used. Of the two closely related Leishmania species of the L. donovani complex that cause VL (L. donovani and L. infantum/chagasi), only the former exists in eastern Africa. However, recent studies employing microsatellite markers have revealed the existence of genetically varied populations of L. donovani in south and north Ethiopia . Whether such genetic variation accounts for the difference in treatment outcomes remains to be determined. Currently ongoing drug susceptibility testing may give some clues. Due to early termination of the study, results were not conclusive regarding the primary objective, i.e. the efficacy comparison of single and multiple doses. However, pharmacodynamic data suggests a benefit of multiple doses on peripheral parasite clearance. On day 3 of treatment, differences in peripheral parasite clearance were significant, even though total administered doses up to this time point were comparable in both arms (10 versus 9 mg/kg, respectively). This suggests a possible role for the frequency of administration. This would need to be confirmed by further studies, but is supported by the relatively high cure rate achieved in single dose patients rescued by additional standard multiple dose regimen. Even in India, where efficacy of single doses was shown to be good, it was lower than multiple dose regimens , . In eastern Africa, there might be no optimal montherapy dosing regimen of AmBisome® for VL but combination therapies including AmBisome® are still of interest and under investigation . In this respect, qRT-PCR results are interesting, although its use in Africa might be hampered by relatively low blood parasitaemia at baseline and the prohibitive cost of the test. More sensitive prognostic pharmacodynamic biomarkers, applicable in all patients, are needed to monitor treatment response.
AmBisome® in single doses of up to 10 mg/kg is suboptimal for eastern African VL patients. A higher dose of 21 mg/kg administered in multiple doses was also far less effective than anticipated. In a small number of patients, no treatment failures occurred in patients from south Ethiopia treated in Arba Minch Hospital either with 10 mg/kg single dose or 21 mg/kg divided doses. The tested AmBisome® regimens would not be suitable for VL treatment across eastern Africa, and an optimal single dose that could be included in shorter and simplified treatment regimens of anti-leishmanial drugs across all VL endemic regions of eastern Africa was not identified. Efficacy of AmBisome®for the treatment of VL in eastern Africa was variable and overall lower than in India.
Our thanks go to: 1) the Food, Medicines, and Health Care Administration & Control Authority (FMHACA) of Ethiopia, 2) the Federal Ministry of Health – Government of Ethiopia, 3) the Ministry of Gedaref state, Sudan, 4) The Regional Health Bureaus in SNNP-RG (South Ethiopia) and Amhara Regional State (north Ethiopia), 5) The College of Health Sciences – Addis Ababa University, and 6) Institute of Endemic Diseases - Sudan. We give a special recognition to the clinical monitors' team and the Data and Safety Monitoring Board.
Conceived and designed the experiments: AH TE EAGK AMM MB MW SE PGS. Performed the experiments: TW BMY WHal AAA ZH SY WHai RK TPCD GJS. Analyzed the data: TE RO SE PGS AH EAGK RK AMM TPCD GJS. Contributed reagents/materials/analysis tools: TPCD GJS TE PGS. Wrote the paper: TE AH EAGK AMM PGS MW AMEH SE.
- 1. Alvar J, Vélez ID, Bern C, Herrero M, Desjeux P, et al. (2012) Leishmaniasis worldwide and global estimates of its incidence. PLoS ONE 7 (5) e35671 doi:10.1371/journal. pone.0035671.
- 2. Hailu A, Musa AM, Royce C, Wasunna M (2005) Visceral leishmaniasis: new health tools are needed. PLoS Med 2: e211. doi: 10.1371/journal.pmed.0020211
- 3. WHO (2007) Fifth Consultative Meeting on Leishmania/HIV Coinfection, Addis Ababa, Ethiopia, 20–22 March 2007. World Health Organisation, WHO/CDS/NTD/IDM/20075.
- 4. Reithinger R, Brooker S, Kolaczinski JH (2007) Visceral leishmaniasis in eastern Africa–current status. Trans R Soc Trop Med Hyg 101: 1169–1170. doi: 10.1016/j.trstmh.2007.06.001
- 5. Zijlstra EE, El-Hassan AM (2001) Leishmaniasis in Sudan. Visceral leishmaniasis. Trans R Soc Trop Med Hyg 95, Suppl 1: S27–S58. doi: 10.1016/s0035-9203(01)90218-4
- 6. Anoopa Sharma D, Bern C, Varghese B, Chowdhury R, Haque R, et al. (2006) The economic impact of visceral leishmaniasis on households in Bangladesh. Trop Med Int Health 11: 757–764. doi: 10.1111/j.1365-3156.2006.01604.x
- 7. Hailu A, Musa A, Wasunna M, Balasegaram M, Yifru S, et al. (2010) Geographical variation in the response of visceral leishmaniasis to paromomycin in East Africa: a multicentre, open-label, randomized trial. PLoS Negl Trop Dis 4: e709. doi: 10.1371/journal.pntd.0000709
- 8. van Griensven J, Balasegaram M, Meheus F, Alvar J, Lynen L, et al. (2010) Combination therapy for visceral leishmaniasis. Lancet Infect Dis 10: 184–194. doi: 10.1016/s1473-3099(10)70011-6
- 9. Bern C, Adler-Moore J, Berenguer J, Boelaert M, den Boer M, et al. (2006) Liposomal amphotericin B for the treatment of visceral leishmaniasis. Clin Infect Dis 43: 917–924. doi: 10.1086/507530
- 10. Sundar S, Sinha PK, Rai M, Verma DK, Nawin K, et al. (2011) Comparison of short-course multidrug treatment with standard therapy for visceral leishmaniasis in India: an open-label, non-inferiority, randomised controlled trial. Lancet 377: 477–486. doi: 10.1016/s0140-6736(10)62050-8
- 11. Minodier P, Retornaz K, Horelt A, Garnier JM (2003) Liposomal amphotericin B in the treatment of visceral leishmaniasis in immunocompetent patients. Fundam Clin Pharmacol 17: 183–188. doi: 10.1046/j.1472-8206.2003.00168.x
- 12. Khalil EAGHF, Mustafa MD, Kordofani A, Satti M, El Hag IA, et al. (1998) A comparative study comparing liposomal amphotericin B (AmBisome) and sodium stibogluconate in the treatment of kala-azar in the Sudan. East Afr Med J 75: 37–45.
- 13. Berman JD, Badaro R, Thakur CP, Wasunna KM, Behbehani K, et al. (1998) Efficacy and safety of liposomal amphotericin B (AmBisome) for visceral leishmaniasis in endemic developing countries. Bull World Health Organ 76: 25–32.
- 14. Edwards T, Omollo R, Khalil EA, Yifru S, Musa B, et al. (2011) Single-dose liposomal amphotericin B (AmBisome(R)) for the treatment of visceral leishmaniasis in East Africa: study protocol for a randomized controlled trial. Trials 12: 66. doi: 10.1186/1745-6215-12-66
- 15. van der Meide W, Guerra J, Schoone G, Farenhorst M, Coelho L, et al. (2008) Comparison between quantitative nucleic acid sequence-based amplification, real-time reverse transcriptase PCR, and real-time PCR for quantification of Leishmania parasites. J Clin Microbiol 46: 73–78. doi: 10.1128/jcm.01416-07
- 16. Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, et al. (1990) Rapid and simple method for purification of nucleic acids. J Clin Microbiol 28: 495–503.
- 17. Basiye FL, Schoone GJ, Beld M, Minnaar R, Ngeranwa JN, et al. (2011) Comparison of short-term and long-term protocols for stabilization and preservation of RNA and DNA of Leishmania, Trypanosoma, and Plasmodium. Diagn Microbiol and Infect Dis 69: 66–73. doi: 10.1016/j.diagmicrobio.2010.08.018
- 18. Sundar S, Jha TK, Thakur CP, Sinha PK, Bhattacharya SK (2007) Injectable paromomycin for Visceral leishmaniasis in India. N Engl J Med 356: 2571–2581. doi: 10.1056/nejmoa066536
- 19. Elnaiem DE (2011) Ecology and control of the sand fly vectors of Leishmania donovani in East Africa, with special emphasis on Phelebotomus orientalis. J Vector Ecol 36 Suppl 1: S23–31 doi:10.1111/j.1948-7134.2011.00109.
- 20. Gelanew T, Kuhls K, Hurissa Z, Weldegebreal T, Hailu W, et al. (2010) Inference of population structure of Leishmania donovani strains isolated from different Ethiopian visceral leishmaniasis endemic areas. PLoS Negl Trop Dis 4: e889. doi: 10.1371/journal.pntd.0000889
- 21. Sundar S, Goyal AK, More DK, Singh MK, Murray HW (1998) Treatment of antimony-unresponsive Indian visceral leishmaniasis with ultra-short courses of amphotericin-B-lipid complex. Ann Trop Med Parasitol 92: 755–764. doi: 10.1080/00034989858998
- 22. Sundar S, Agrawal G, Rai M, Makharia MK, Murray HW (2001) Treatment of Indian visceral leishmaniasis with single or daily infusions of low dose liposomal amphotericin B: randomised trial. BMJ 323: 419–422. doi: 10.1136/bmj.323.7310.419
- 23. Omollo R, Alexander N, Edwards T, Khalil EA, Younis BM, et al. (2011) Safety and efficacy of miltefosine alone and in combination with sodium stibogluconate and liposomal amphotericin B for the treatment of primary visceral leishmaniasis in East Africa: study protocol for a randomized controlled trial. Trials 12: 166 Doi: 10.1186/1745-6215-12-166. doi: 10.1186/1745-6215-12-166