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Research Article

Use of a Synthetic Biosensor for Neutralizing Activity-Biased Selection of Monoclonal Antibodies against Atroxlysin-I, an Hemorrhagic Metalloproteinase from Bothrops atrox Snake Venom

  • Francisco Santos Schneider,

    Affiliation: Departamento de Bioquímica e Imunologia, Instituto Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil

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  • Dung Le Nguyen,

    Affiliation: SysDiag, UMR3145,CNRS/BioRad, Montpellier, France

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  • Karen Larissa Castro,

    Affiliation: Departamento de Bioquímica e Imunologia, Instituto Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil

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  • Sandra Cobo,

    Affiliation: SysDiag, UMR3145,CNRS/BioRad, Montpellier, France

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  • Ricardo Andrez Machado de Avila,

    Affiliation: Departamento de Bioquímica e Imunologia, Instituto Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil

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  • Nivia de Assis Ferreira,

    Affiliation: Departamento de Bioquímica e Imunologia, Instituto Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil

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  • Eladio Flores Sanchez,

    Affiliation: Departamento de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias, Belo Horizonte, Brasil

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  • Christophe Nguyen,

    Affiliation: SysDiag, UMR3145,CNRS/BioRad, Montpellier, France

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  • Claude Granier,

    Affiliation: SysDiag, UMR3145,CNRS/BioRad, Montpellier, France

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  • Pascale Galéa,

    Affiliation: SysDiag, UMR3145,CNRS/BioRad, Montpellier, France

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  • Carlos Chávez-Olortegui mail,

    olortegi@icb.ufmg.br

    Affiliation: Departamento de Bioquímica e Imunologia, Instituto Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil

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  • Franck Molina

    Affiliation: SysDiag, UMR3145,CNRS/BioRad, Montpellier, France

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  • Published: April 24, 2014
  • DOI: 10.1371/journal.pntd.0002826

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