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Figure 3. Visual Appearance of Postassay SRA LAMP Reactions from Isolates after the Addition of 1 µl of 1/10 Dilution of SYBR Green I.

Positive samples produce a green colour almost immediately (tubes 1, 2, and 3 containing 100 pg of T. b. rhodesiense DNA) while negatives remain orange (tube 4 and NC, negative control).